1972
DOI: 10.1073/pnas.69.9.2566
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Method for Correlation of Proton Magnetic Resonance Assignments in Different Solvents: Conformational Transition of Oxytocin and Lysine Vasopressin from Dimethylsulfoxide to Water

Abstract: The peptide amide, primary carboxamide, and aromatic proton resonances were assigned to specific hydrogens of oxytocin and lysine vasopressin (Lys-VP) in water at 230 at pH 2.5 and 4.2, respectively. We started with the spectral assignments of oxytocin and Lys-VP determined in deuterated dimethylsulfoxide (Me2SO) and monitored the course of each of these resonances as the proportion of water to Me2SO was gradually increased. Changes in each of the two hormones in chemical shifts and in some coupling constants … Show more

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Cited by 33 publications
(21 citation statements)
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“…This resonance can therefore be assigned unambiguously to the tyrosine NH proton. This is in agreement with the results of both Walter and coworkers [3] and Brewster and Hruby [4]. However, both these groups assigned this resonance essentially by elimination, since the doubleresonance experiment in H20 was inconclusive [4], and the resonance disappeared in DMSO/water mixtures containing between 30% and 80% water [3].…”
Section: Resultssupporting
confidence: 91%
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“…This resonance can therefore be assigned unambiguously to the tyrosine NH proton. This is in agreement with the results of both Walter and coworkers [3] and Brewster and Hruby [4]. However, both these groups assigned this resonance essentially by elimination, since the doubleresonance experiment in H20 was inconclusive [4], and the resonance disappeared in DMSO/water mixtures containing between 30% and 80% water [3].…”
Section: Resultssupporting
confidence: 91%
“…1 b) shows clearly that the NH resonance at 7.94 ppm in oxytocin is that of isoleucine. This assignment is in agreement with that of Brewster and Hruby [4], but not with that of Walter et al [3]. The second type of isotopic substitution we have employed for assignment purposes is exemplified by 8-[a-2 H-Leu]-oxytocin ( fig.…”
Section: Resultssupporting
confidence: 88%
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