Method for determination of (-102C>T) single nucleotide polymorphism in the human manganese superoxide dismutase promoter

Abstract

Background: Manganese superoxide dismutase (MnSOD) plays a critical role in the detoxification of mitochondrial reactive oxygen species constituting a major cellular defense mechanism against agents that induce oxidative stress. The MnSOD promoter contains an activator protein-2 (AP-2) binding site that modifies transcription of MnSOD. Mutations have been identified in the proximal region of the promoter in human tumor cell lines. One of these mutations (-102C>T) has been shown to change the binding pattern of AP-2 leading to a reduction in transcriptional activity. The aim of our study was to develop a method to identify and determine the frequency of this (-102C>T) polymorphism in human tissues.

Manganese Superoxide Dismutase Expression as a Function of Genotype and Lung Cancer Pathology

“…The −9 T>C and −102 C>T MnSOD polymorphisms were determined by a TaqMan allelic discrimination assay as recently published(10;11). SNP-specific polymerase chain reaction (PCR) primers and fluorogenic probes were designed using Primer Express (Version 1.5; Applied Biosystems, Foster City, CA).…”