Method for efficiently obtaining fertilized eggs from the black scraper Thamnaconus modestus by natural spawning in captivity

Mizuno, Kaori; Shimizu-Yamaguchi, Sonoko; Miura, Chiemi; Miura, Takeshi

doi:10.1007/s12562-012-0527-z

Cited by 11 publications

(5 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…P. olivaceus and S. japonica are benthic fish which inhabit in the sandy zone, and T. alboplumbeus commonly feed on the sandy area and often burrow in the sand substrate (Katayama et al, 1966;Yamahira et al, 1996). While T. modestus is not benthic fish nor shows burrowing behavior, they often occur near the bottom, feed and spawn at the sandy sea bed (Kim et al, 2013;Mizuno et al, 2012). In the survey conducted during 2012-2013 for 5 fishes (Lateolabrax japonicus, Pagrus major, Seriola quinqueradiata, Seriola dumerili, and Takifugu rubripes) cultured in sea cages at and near the endemic area of K. septempunctata found no infection by PCR and microscopy (Dr. Azumi Yamashita, personal communication).…”

Section: Discussionmentioning

confidence: 99%

Infections of Kudoa septempunctata (Myxozoa: Multivalvulida) in Wild Grass Puffer Takifugu alboplumbeus and Japanese Whiting Sillago japonica

et al. 2021

View full text Add to dashboard Cite

though the case number has lowered. These recent incidences are thought to be associated with infected wild-and imported farmed P. olivaceus. Despite the recent expansion of Kudoa research, our knowledge about the general biology of this parasite is limited, and this hinders the establishment of a fundamental measure to prevent the infection in farmed fish.To date, the lifecycle of K. septempunctata, or for any Kudoa species, has not been elucidated. Neither fish infective stage (actinospore) nor invertebrate alternate host has been discovered for even a single Kudoa species. Furthermore, the host range of K. septempunctata remains unclear. While K. septempunctata is generally considered the parasite of a pleuronectiform P. olivaceus, it has also been recorded from black scrapers T h a m n a c o n u s m o d e s t u s ( T e t r a o d o n t i f o r m e s ; Monacanthidae) by Kasai et al. (2016). They examined 51 wild T. modestus and found 2 infected individuals, one captured in the Inland Sea of Japan and another one from the Sea of Japan. This indicates that K. septempunctata may have a wide host range and fishes other than P. olivaceus may serve as its hosts in the wild. To deepen the understanding of the biology of K. septempunctata and to elucidate its lifecycle, we have Kudoa septempunctata (Myxozoa: Multivalvulida) is the first recognized food poisoning myxosporean parasite (Kawai et al., 2012;Sugita-Konishi et al., 2014). This myxozoa infects the trunk muscle of olive flounder Paralichthys olivaceus and the consumption of raw flounder may induce transient vomiting and diarrhea. The parasite has caused the number of food poisoning cases in Japan, up to over 40 annual cases with more than 450 patients (Japanese Ministry of Health, Labour and Welfare). These Kudoa-related gastroenteritis were initially attributed largely to the distribution of infected farmed flounder in the market (Yahata et al., 2015). In recent years, however, K. septempunctata infection in farmed P. olivaceus has essentially become under control in Japan, as a result of the tireless research and the development of countermeasures. The control measures involve periodic inspections of the parasite in flounder hatcheries and farms, as well as the disinfection and parasite elimination of rearing water (Nishioka et al., 2016). Despite such accomplishments, food poisoning by K. septempunctata still occurs today,

show abstract

Section: Discussionmentioning

confidence: 99%

Infections of Kudoa septempunctata (Myxozoa: Multivalvulida) in Wild Grass Puffer Takifugu alboplumbeus and Japanese Whiting Sillago japonica

et al. 2021

View full text Add to dashboard Cite

show abstract

“…The black scraper, Thamnaconus modestus (Monacanthidae: Tetraodontiformes), is widely distributed in southern China, the coastal waters of South Korea, and Hokkaido of Japan, and is a highly valued marine fish (Kim, 2007;Mizuno et al, 2012;Kim et al, 2013;Ying et al, 2022). It inhabits a depth range of 50-110 m within a temperature range of 10-28°C and feeds primarily on plankton and benthic organisms (Choi et al, 2002;Kim, 2007).…”

Section: Introductionmentioning

confidence: 99%

“…Furthermore, due to overexploitation, increasing demand, and limited biological information, this amount has gradually decreased, and it has been listed on the International Union for Conservation of Nature Red List of Threatened Species (IUCN, 2023). To increase the output, great efforts have been made to promote the technological development of artificial reproduction and seedling production globally (Arcand-Hoy and Benson, 1998;Kawase, 2002;Mizuno et al, 2012;Kim et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Changes in growth, morphology, and levels of digestive enzymes and growth-related hormones in early ontogeny of black scraper, Thamnaconus modestus

Liu,

Zeng,

Zhang

et al. 2024

Front. Mar. Sci.

View full text Add to dashboard Cite

IntroductionThe black scraper, Thamnaconus modestus, is a highly valued marine fish species, but its output has gradually decreased in recent years, which may be due to its low survival rate during early ontogenesis.MethodsTherefore, in this study, we assessed the changes in growth, morphology, digestive enzymes, and hormone levels in T. modestus from 0–60 days post-hatching (dph) and revealed growth turning points by morphological measurement and determination of digestive enzyme activities and hormone levels. We found that ontogenesis could be divided into the larval (0–20 dph) and juvenile (20–60 dph) stages. Acid and alkaline protease activity significantly increased and decreased, respectively, from 12–25 dph, likely due to the development of stomach and gastric glands. Acid phosphatase levels significantly increased at 0 and 4 dph, which may be related to the regulation of metabolism and immune protection. A sharp increase in alkaline phosphatase levels at 20 and 25 dph was observed and was likely due to the development of the brush border membrane of enterocytes. The amylase level was significantly higher at 25, 30, and 35 dph, possibly due to better digestion and absorption during the transition from consuming Artemia to compound feed. In newly hatched larvae, the level of thyroid hormones triiodothyronine (T3) and thyroxine (T4) gradually increased and peaked at 35 dph, highlighting the importance of these hormones during the development of T. modestus. Growth hormone (GH) levels first increased from 0–8 dph, with a plateau at 8–20 dph, and then increased at 25–30–35 dph. For insulin-like growth factor 1 (IGF-1), a significant increase with a subsequent plateau was observed between 8 and 20 dph, followed by a substantial decrease between 30 and 35 dph. These results suggest that the regulating functions of GH and IGF-1 are synchronised. Digestive enzyme activity and hormone levels of abnormal fry at 30 dph were lower than those of normal fish, highlighting the importance of specific hormones, especially T4 and IGF-1, in the development of T. modestus.

show abstract

“…In subsequent years, researchers have attempted to explore methods to properly culture filefish. Several key technologies, including fertilized egg collection, sperm cryopreservation, larval rearing, and tank and cage culturing, have been studied, and this species is cultivated commercially in China, Korea and Japan (Guan et al, 2013;Kang et al, 2004;Li, Jiang, Xu, & Liu, 2002;Liu et al, 2017;Mizuno, Shimizu-Yamaguchi, Miura, & Miura, 2012). Aquaculture practices for this species can also be improved by genomics data, which facilitate genome-wide association studies (GWAS) to identify DNA markers and causative genes associated with traits of interest (Yue & Wang, 2017).…”

Section: Introductionmentioning

confidence: 99%

Chromosome‐level genome assembly of the greenfin horse‐faced filefish (Thamnaconus septentrionalis) using Oxford Nanopore PromethION sequencing and Hi‐C technology

Bian

et al. 2020

Molecular Ecology Resources

View full text Add to dashboard Cite

The greenfin horse-faced filefish, Thamnaconus septentrionalis, is a valuable commercial fish species that is widely distributed in the Indo-West Pacific Ocean. This fish has characteristic blue-green fins, rough skin and a spine-like first dorsal fin. Thamnaconus septentrionalis is of conservation concern because its population has declined sharply, and it is an important marine aquaculture fish species in China. Genomic resources for the filefish are lacking, and no reference genome has been released. In this study, the first chromosome-level genome of T. septentrionalis was constructed using nanopore sequencing and Hi-C technology. A total of 50.95 Gb polished nanopore sequences were generated and were assembled into a 474.31-Mb genome, accounting for 96.45% of the estimated genome size of this filefish. The assembled genome contained only 242 contigs, and the achieved contig N50 was 22.46 Mb, a surprisingly high value among all sequenced fish species. Hi-C scaffolding of the genome resulted in 20 pseudochromosomes containing 99.44% of the total assembled sequences. The genome contained 67.35 Mb of repeat sequences, accounting for 14.2% of the assembly. A total of 22,067 protein-coding genes were predicted, 94.82% of which were successfully annotated with putative functions. Furthermore, a phylogenetic tree was constructed using 1,872 single-copy orthologous genes, and 67 unique gene families

show abstract

Method for efficiently obtaining fertilized eggs from the black scraper Thamnaconus modestus by natural spawning in captivity

Cited by 11 publications

References 12 publications

Infections of <i>Kudoa septempunctata</i> (Myxozoa: Multivalvulida) in Wild Grass Puffer <i>Takifugu alboplumbeus</i> and Japanese Whiting <i>Sillago japonica</i>

Infections of <i>Kudoa septempunctata</i> (Myxozoa: Multivalvulida) in Wild Grass Puffer <i>Takifugu alboplumbeus</i> and Japanese Whiting <i>Sillago japonica</i>

Changes in growth, morphology, and levels of digestive enzymes and growth-related hormones in early ontogeny of black scraper, Thamnaconus modestus

Chromosome‐level genome assembly of the greenfin horse‐faced filefish (Thamnaconus septentrionalis) using Oxford Nanopore PromethION sequencing and Hi‐C technology

Contact Info

Product

Resources

About