Method for Gradient Elution in Micro-Flow Liquid Chromatography

Aspects and applications of non-aqueous high temperature packed capillary liquid chromatography

“…[3][4][5][6][7][8][9][10] Traditional solvent gradient elution has been reported to be difficult with columns of small id, due to the small flow rates required. 11 However, the smaller dimensions of packed capillary columns make them especially well suited for temperature programming, due to their small thermal mass. 12 Hence, temperature-programmed LC has in some studies proven to be an alternative to solvent gradient elution when utilizing packed capillary columns.…”

Separation of Ceramides by Sub-Ambient Temperature-Assisted Large Volume Injection in Temperature-Programmed Packed Capillary Liquid Chromatography

“…Solvent gradient elution is the traditional way to adjust the elution strength of the mobile phase in LC during the chromatographic run. However, this technique is not simple with columns of small inner diameters, due to instrumental limitations with regard to the low flow rates required [8]. Packed capillary columns are, however, well suited for temperature programming, due to their low thermal mass [9].…”

Temperature-Programmed Packed Capillary Liquid Chromatography Coupled to Fourier-Transform Infrared Spectroscopy

“…Solvent gradient elution is the traditional way to adjust the elution strength of the mobile phase in LC during the chromatographic run. However, this technique is not simple with columns of small inner diameters, due to instrumental limitations with regard to the low flow rates required [11]. In addition, gradual changes of the mobile phase composition can have a negative influence on nebulization processes, such as in LC-FT-IR.…”