Method for the simultaneous determination of losartan and its major metabolite, EXP-3174, in human plasma by liquid chromatography–electrospray ionization tandem mass spectrometry

Iwasa, Takashi; Takano, Terukazu; Hara, Kazushi; Kamei, Tosio

doi:10.1016/s0378-4347(99)00358-8

Cited by 45 publications

(26 citation statements)

References 9 publications

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“…The plasma levels of the two drugs have been found at lower level and shorter duration than previously reported. 31) The developed method could be efficiently used in losartan therapeutic drug monitoring in clinical situation.…”

Section: Discussionmentioning

confidence: 99%

Pre-treatment with Curcumin Enhances Plasma Concentrations of Losartan and Its Metabolite EXP3174 in Rats

Liu

Zhao

Xing

et al. 2012

Biological & Pharmaceutical Bulletin

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The study was carried out in the Wistar rats to investigate the effect of curcumin pre-treatment on the pharmacokinetics of the hypertension-treating drug losartan and its metabolite EXP3174 following single oral administration. In the treatment group, rats were gavaged with losartan 10 mg/kg after repeat oral doses of curcumin (100 mg/kg, for 7 d), while rats in the control group were administrated only with the same dose losartan. The results showed that curcumin significantly increased the plasma concentrations of losartan and its metabolite EXP3174. The present study implicated the existence of herb-drug interaction between curcumin and losartan, and further evaluation of the possible interaction during curcumin administration needs to be considered.

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Section: Discussionmentioning

confidence: 99%

Pre-treatment with Curcumin Enhances Plasma Concentrations of Losartan and Its Metabolite EXP3174 in Rats

Liu

Zhao

Xing

et al. 2012

Biological & Pharmaceutical Bulletin

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“…Therefore, the method of extraction of LOS and LA from plasma was rugged enough and gave accurate and consistent results when applied to real study samples. In the earlier LC-MS/MS method published, the investigators pointed lower recoveries to suppression at the ion source; however, the matrix effect was not evaluated [11].…”

Section: Methods Validationmentioning

confidence: 99%

“…(ii) Present method is simple and rapid SPE technique without drying and reconstitution steps. SPE methods with drying and reconstitution [11,13], liquidliquid extraction also with drying and reconstitution [12], and protein precipitation [14] methods have been reported in literature. (iii) Current method has LLOQ (lower LOQ) 2.5 ng/mL and LOD 25 pg/mL for LOS and LLOQ 5 ng/mL and LOD 50 pg/mL for LA.…”

Section: Introductionmentioning

confidence: 99%

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Rapid determination of losartan and losartan acid in human plasma by multiplexed LC–MS/MS

Shah

Kundlik

Patel

et al. 2009

J of Separation Science

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A rapid LC-MS/MS method has been developed and validated for the determination of losartan (LOS) and its metabolite losartan acid (LA) (EXP-3174) in human plasma using multiplexing technique (two HPLC units connected to one MS/MS). LOS and LA were extracted from human plasma by SPE technique using Oasis HLB cartridge without evaporation and reconstitution steps. Hydroflumethiazide (HFTZ) was used as an internal standard (IS). The analytes were separated on Zorbax SB C-18 column. The mass transition [M-H] ions used for detection were m/z 421.0 --> 127.0 for LOS, m/z 435.0 --> 157.0 for LA, and m/z 330.0 --> 239.0 for HFTZ. The proposed method was validated over the concentration range of 2.5-2000 ng/mL for LOS and 5.0-3000 ng/mL for LA with correlation coefficient > or = 0.9993. The overall recoveries for LOS, LA, and IS were 96.53, 99.86, and 94.16%, respectively. Total MS run time was 2.0 min/sample. The validated method has been successfully used to analyze human plasma samples for applications in 100 mg fasted and fed pharmacokinetic studies.

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“…5 In biological fluids, the active principle as well as its metabolites have been determined by HPLC, UV detection, 6 fluorescence detection, 7 and liquid chromatography electrospray ionization tandem mass spectrometry. 8 Triamterene (2,4,7-triamino-6-phenylpteridine) (Scheme 1) is a mild diuretic belonging to the potassium-saving family, which acts directly on distal tubular cells to diminish the release of potassium ions and to avoid the reassertion of chloride ions as a result. By itself, it is relatively inefficient as a hypertensive, so it is usually associated with some other, more potent diuretic (e.g.…”

mentioning

confidence: 99%

Determination of Losartan and Triamterene in Pharmaceutical Compounds and Urine Using Cathodic Adsorptive Stripping Voltammetry

Ensafi

Hajian

2008

ANAL. SCI.

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Method for the simultaneous determination of losartan and its major metabolite, EXP-3174, in human plasma by liquid chromatography–electrospray ionization tandem mass spectrometry

Cited by 45 publications

References 9 publications

Pre-treatment with Curcumin Enhances Plasma Concentrations of Losartan and Its Metabolite EXP3174 in Rats

Pre-treatment with Curcumin Enhances Plasma Concentrations of Losartan and Its Metabolite EXP3174 in Rats

Rapid determination of losartan and losartan acid in human plasma by multiplexed LC–MS/MS

Determination of Losartan and Triamterene in Pharmaceutical Compounds and Urine Using Cathodic Adsorptive Stripping Voltammetry

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