Method to Improve Azo-Compound (AAPH)-Induced Hemolysis of Erythrocytes for Assessing Antioxidant Activity of Lipophilic Compounds

Nuruki, Yusuke; Matsumoto, H.; Tsukada, Miho; Tsukahara, Haruka; Takajo, Tokuko; Tsuchida, Kazunori; Anzai, Kazunori

doi:10.1248/cpb.c20-00568

Cited by 14 publications

(8 citation statements)

References 38 publications

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“…AAPH is a prooxidant compound that causes the oxidation of hemoglobin to methemoglobin, consequently inducing hemolysis, and possibly affecting the lipophilic structure of the erythrocyte membrane [ 57 ]. The synthesized AgNPs were more active compared to the extract.…”

Section: Resultsmentioning

confidence: 99%

“…However, both the extract and AgNPs can block the prooxidant and hemolytic action of AAPH, without causing hemolysis in its absence. Blocking AAPH activity is done mainly by compounds that have both hydrophilic groups, that are capable of interacting with AAPH, but also lipophilic structures that allow passage through the erythrocyte membrane and block the intracellular action of AAPH [ 57 ]. Such compounds also have a protective effect on subcellular structures, proteins and DNA and can, thus, block pathological phenomena caused by oxidative stress [ 58 ].…”

Section: Resultsmentioning

confidence: 99%

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Green Synthesis and Characterization of Silver Nanoparticles Using a Lythrum salicaria Extract and In Vitro Exploration of Their Biological Activities

Corciovă

Mircea

Burlec

et al. 2022

Life

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This research describes an eco-friendly green route for the synthesis of AgNPs using an aqueous extract of Lythrum salicaria. Taguchi design was used to optimize the synthesis method, taking into account various working conditions. The optimum parameters were established using a 3 mM AgNO3 concentration, a 1:9 extract:AgNO3 volume ratio, a pH value of 8, 60 ℃ temperature, and 180 min reaction time. The synthesized AgNPs were characterized using UV-Vis and FTIR spectroscopy, and TEM and EDX analysis. The SPR band at 410 nm, as well as the functional groups of biomolecules identified by FTIR and the EDX signals at ~3 keV, confirmed the synthesis of spherical AgNPs. The average AgNPs size was determined to be 40 nm, through TEM, and the zeta potential was −19.62 mV. The antimicrobial assay showed inhibition against S. aureus and C. albicans. Moreover, the results regarding the inhibition of lipoxygenase and of peroxyl radical-mediated hemolysis assays were promising and justify further antioxidant studies.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Green Synthesis and Characterization of Silver Nanoparticles Using a Lythrum salicaria Extract and In Vitro Exploration of Their Biological Activities

Corciovă

Mircea

Burlec

et al. 2022

Life

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“…By measuring the optical absorbance given by the liberated haemoglobin in the supernatant, the extent of hemolysis for the controls and exposed samples was indirectly measured. In vitro hemolysis study was carried out using procedure reported by Nuruki et al 26 Hemolysis of phytosomal formulations was studied at high (1000 µg/ml), medium (100 µg/ml), and low (10 µg/ml) concentrations, using pure SL as a control. To obtain clear cells, blood from a healthy volunteer was centrifuged at 1500g for 20 min and rinsed three times with normal saline.…”

Section: = ×mentioning

confidence: 99%

Development and Characterization of Silymarin Phospholipid Complex for Improved Solubility, and Toxicological Evaluation in Experimental Animals

Singh

Patil

2022

IJPI

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Objectives:The silymarin phytosome (SPY) was developed to improve the solubility and dissolution. The prepared phytosome was evaluated for hemolytic, membrane stabilization activity, and in vivo acute and subchronic toxicity. Materials and Methods: The toxicological evaluation was carried out by assessing its effects on liver, kidney, brain, lungs, heart and spleen in a rat model. In addition to confirmation of formation of phytosome, the physical-chemical characterization was done by FTIR, DSC, PXRD, and 1H NMR. Results: The optimized formulation has higher aqueous solubility of SPY compared to that of pure silymarin. The formulation also exhibited a significantly higher rate and extent of silymarin release in dissolution studies. The percent hemolysis for SPY was significantly less than silymarin. As a result, SPY can be concluded to be non-toxic and biocompatible for in vivo administration. SPY significantly inhibited hypotonic solutioninduced hemolysis in a dose-dependent manner. In acute oral toxicity, no treatment-related death or toxic signs were observed. The sub-acute test observations indicated that there are no treatment-related changes up to the high dose level. Food consumption, body weight, organ weight, hematological parameters, biochemical parameters and histopathological examination revealed no abnormalities. Conclusion: The study shows that SPY is a promising and viable strategy for improving the delivery of silymarin.

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“…In the second investigation free radicals were induced by AAPH thermal decomposition in aqueous conditions. Sekiya et al 19 measured AAPH-induced hemolysis at 540 nm which, according to Nuruki et al 38 was not the optimal wavelength, because AAPH oxidizes the oxygenated hemoglobin to methemoglobin and absorbance at 540 nm does not correctly reflect the amount of released hemoglobin by AAPH-induced hemolysis. Thus, the absorbance at 523 nm correctly reflects the amount of released hemoglobin regardless of the status of hemoglobin (isosbestic point).…”

Section: O N L I N E F I R S Tmentioning

confidence: 99%

“…Thus, the absorbance at 523 nm correctly reflects the amount of released hemoglobin regardless of the status of hemoglobin (isosbestic point). 38…”

Section: O N L I N E F I R S Tmentioning

confidence: 99%

DNA protective activity of triterpenoids isolated from medicinal mushroom Fomitopsis betulina

Sofrenic

Andjelkovic

Vujisić

et al. 2021

JSCS

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Eleven 31-methylenlanostane triterpenoids, i.e. seven 21- and four 26-oic acids, as well as a lupane triterpenoid betulin, isolated from the fruiting bodies of the mushroom Fomitopsis betulina were tested for in vitro protective effect on chromosome aberrations in peripheral human lymphocytes using cytochalasin-B blocked micronucleus (CBMN) assay. Most of the tested compounds exerted a beneficial effect by reducing DNA damage of human lymphocytes more effectively than amifostine, a radioprotective agent, used as a positive control. All the tested compounds decreased MN frequency in concentration dependent manner, with the concentration of 2.0 ?g mL-1 being the most effective - with increase of the concentration the activity slightly decreases. The structure-activity relationship (SAR) studies indicated that the lanostanes containing a conjugated 7,9 (11)-diene system exhibit lower activity than D8-analogues. It was also demonstrated that the DNA protective activities within the D8-lanostane-26-oic acid group are affected by 3-substitution pattern. In the D8 series the oxygenation at C-12 or 16 as well as 21- or 26-oic acid functionality proved beneficial for in vitro protective effect on chromosomal aberrations. Betulin exhibited the lowest protective activity, but still comparable to that of amifostine.

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Method to Improve Azo-Compound (AAPH)-Induced Hemolysis of Erythrocytes for Assessing Antioxidant Activity of Lipophilic Compounds

Cited by 14 publications

References 38 publications

Green Synthesis and Characterization of Silver Nanoparticles Using a Lythrum salicaria Extract and In Vitro Exploration of Their Biological Activities

Green Synthesis and Characterization of Silver Nanoparticles Using a Lythrum salicaria Extract and In Vitro Exploration of Their Biological Activities

Development and Characterization of Silymarin Phospholipid Complex for Improved Solubility, and Toxicological Evaluation in Experimental Animals

DNA protective activity of triterpenoids isolated from medicinal mushroom Fomitopsis betulina

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