Method validation and determinations of levofloxacin, metronidazole and sulfamethoxazole in an aqueous pharmaceutical, urine and blood plasma samples using quantitative nuclear magnetic resonance spectrometry

Salem, Alaa A.; Mossa, H.A.

doi:10.1016/j.talanta.2011.10.016

Cited by 35 publications

(16 citation statements)

References 50 publications

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“…LEV containing pharmaceuticals are commonly used for the treatment of respiratory tract infections, skin infections and genitourinary tract infections . Quantitative determination of LEV has been studied by several analytical techniques such as spectrofluorimetry , nuclear magnetic resonance spectrometry , high‐performance liquid chromatography coupled with MS/MS , high performance liquid chromatography with photodiode array detector , UV spectrophotometric method and electrochemical technique . Nevertheless, the electrochemical methods are better than these mentioned techniques due to ease of operation, low cost, quick response time and high sensitivity.…”

Section: Introductionsupporting

confidence: 93%

Poly(Taurine‐Glutathione)/Carbon Nanotube Modified Glassy Carbon Electrode as a New Levofloxacin Sensor

Koçak

2019

Electroanalysis

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A new highly sensitive and selective electrochemical levofloxacin sensor based on co‐polymer‐carbon nanotube composite electrode was developed. Taurine and Glutathione were electrochemically co‐polymerized on multiwalled carbon nanotubes modified glassy carbon electrode (Poly(TAU‐GSH)/CNT/GCE) and used as a levofloxacin sensor in pH 6 phosphate buffer solution. The new composite electrode surfaces were characterized by scanning electron microscopy, atomic force microscopy and electrochemical impedance spectroscopy. Under the optimized conditions, two linear segments were obtained for increasing LEV concentrations between 20 nmol L−1‐1 μmol L−1 and 1.5 μmol L−1‐55 μmol L−1 LEV with a detection limit of 9 nmol L−1 using amperometry. Poly(TAU‐GSH)/CNT/GCE exhibited high sensitivity, selectivity with good stability. The new sensor was employed for real samples of LEV tablets and urine. Promising results were obtained with good accuracy which were also in accordance with LC‐MS/MS analysis.

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Section: Introductionsupporting

confidence: 93%

Poly(Taurine‐Glutathione)/Carbon Nanotube Modified Glassy Carbon Electrode as a New Levofloxacin Sensor

Koçak

2019

Electroanalysis

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“…Though it has a lower sensitivity relative to HPLC and mass spectrometry (MS) [ 3 ], the quantitative NMR (qNMR) method possesses distinctive advantages: (1) it can be conducted without analyte reference materials; (2) it can provide structural information and does not destroy the samples; (3) it can undertake multicomponent analysis in a mixture without pre-isolation; and (4) it requires a comparatively short time [ 4 , 5 , 6 , 7 , 8 , 9 ]. Nowadays, qNMR is mainly applied to identify and quantify drugs, biological metabolites, and natural products [ 10 , 11 , 12 , 13 , 14 , 15 , 16 ]. The basis of qNMR is the proportional relationship between the given integral resonance and the number of protons.…”

Section: Introductionmentioning

confidence: 99%

The Application of Quantitative 1H-NMR for the Determination of Orlistat in Tablets

et al. 2017

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A quantitative nuclear magnetic resonance (qNMR) method to measure the content of Orlistat in tablets was studied and found to be efficient, accurate, reliable, and simple. In this paper, phloroglucinolanhydrous and dimethylsulfoxide-d6 (DMSO-d6) served as the internal standard and solvent, respectively. The qNMR methodology, including the linearity, range, the limit of detection (LOD) and quantification (LOQ), stability, precision, and accuracy, was validated seriatim, and the results were very favorable. The content determination results of three batches of Orlistat in tablets were almost identical upon comparing the qNMR method and the high-performance liquid chromatography (HPLC) method. The recommended method authentically compensated the deficiencies of the current HPLC method for determining Orlistat content, and proved to be a method complementary to traditional analysis for the purity measurement of Orlistat in some pharmaceutical preparations.

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“…All aforementioned techniques require proper sample preconditioning. The following matrices may be used: serum [ 26 , 43 ], plasma [ 20 , 21 , 23 , 24 , 27 , 28 , 31 , 32 , 34 , 45 ], blood [ 30 ], urine [ 23 , 38 , 43 – 45 ], sputum [ 36 ], tissues [ 33 – 35 ], bone [ 21 ], bronchoalveolar lavage (BAL) [ 21 ], bile [ 30 ], water [ 40 ], dialysate [ 28 ], reaction mixture [ 19 ], and pharmaceutical formulation [ 18 , 22 , 25 , 39 , 41 – 43 , 45 , 46 ]. For protein precipitation, an extraction step, dilution is typically applied prior to analysis.…”

Section: Third Generation Fluoroquinolonesmentioning

confidence: 99%

Analytical Methods for Determining Third and Fourth Generation Fluoroquinolones: A Review

Czyrski

2016

Chromatographia

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Fluoroquinolones of the third and fourth generation posses wide bactericidal activity. Monitoring concentrations of antibacterial agents provides effective therapy and prevents the increase of bacterial resistance to antibiotics. The pharmacodynamic parameters that best describe fluoroquinalone activity are AUC/MIC and C max/MIC. Determining the level of this type of drug is essential to reach the effective concentration that inhibits the growth of bacteria. Determining the pharmaceutical formulation confirms the purity of a substance. Many methods have been developed to determine the level of these substances. They involve mainly the following analytical techniques: chromatography, capillary electrophoresis, and spectroscopy. The separation techniques were combined with different measuring devices, such as ultraviolet (UV), fluorescence detector (FLD), diode array detector (DAD), and mass spectrometry (MS). The analytical procedures require proper sample pre-conditioning such as protein precipitation, extraction techniques, filtration, or dilution. This paper reviews the reported analytical methods for the determining representatives of the third and fourth generation of fluoroquinolones. Attention was paid to pre-conditioning of the samples and the applied mobile phase. This report might be helpful in the selection of the proper procedure in determining the abovementioned drugs in different matrices.Graphical abstract

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Method validation and determinations of levofloxacin, metronidazole and sulfamethoxazole in an aqueous pharmaceutical, urine and blood plasma samples using quantitative nuclear magnetic resonance spectrometry

Cited by 35 publications

References 50 publications

Poly(Taurine‐Glutathione)/Carbon Nanotube Modified Glassy Carbon Electrode as a New Levofloxacin Sensor

Poly(Taurine‐Glutathione)/Carbon Nanotube Modified Glassy Carbon Electrode as a New Levofloxacin Sensor

The Application of Quantitative 1H-NMR for the Determination of Orlistat in Tablets

Analytical Methods for Determining Third and Fourth Generation Fluoroquinolones: A Review

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