Method Validation for the Determination of Furosemide in Plasma by Liquid- Liquid Extraction and High-Performance Liquid Chromatography With Fluorescence Detection

G, Carolina Gomez; R, Carlos von Plessing; H, Rolando Reinbach; R, Ricardo Godoy

doi:10.4067/s0717-97072005000200008

Cited by 8 publications

(6 citation statements)

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“…Although UV spectrometric detection has been used in the early nineties [2,3], it seems that fluorescence detection was preferred for furosemide together with the ion pair separation mechanism [4,5]. Mass spectrometry (MS) and tandem mass spectrometry (MS/MS) have also been used to determine furosemide in biological samples, more often by applying negative ion monitoring and atmospheric pressure chemical ionization (APCI) [6,7].…”

Section: Introductionmentioning

confidence: 99%

Analytical issues in HPLC/MS/MS simultaneous assay of furosemide, spironolactone and canrenone in human plasma samples

Sora¹,

Udrescu²,

Albu³

et al. 2010

Journal of Pharmaceutical and Biomedical Analysis

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Section: Introductionmentioning

confidence: 99%

Analytical issues in HPLC/MS/MS simultaneous assay of furosemide, spironolactone and canrenone in human plasma samples

Sora¹,

Udrescu²,

Albu³

et al. 2010

Journal of Pharmaceutical and Biomedical Analysis

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“…The drug also decreases reabsorption of sodium and chloride and increases potassium excretion in the distal renal tubule and exerts a direct effect on electrolyte transport at the proximal tubule (Uchida et al, 1991;McCurley et al, 2004;Buffin-Meyer et al, 2004). sample acidification, deproteinization steps or internal standard addition is ethyl acetate (Gomez et al, 2005;Jankowski et al, 1997;Reeuwijk et al, 1992;Abdel-Hamid, 2000). Other mentioned solvents are diethyl ether (Abou-Auda et al, 1998) and dichloromethane (Takamura et al, 1997).…”

Section: Introductionmentioning

confidence: 99%

“…Most of them are based on the reversed-phase mechanism using octadecylsilicagel as stationary phase; other types of encountered silica modifications are octyl (Singh et al, 1989) and cyano (Abdel-Hamid, 2000). As mobile phases, mixtures of aqueous-polar organic solvent (usually acetonitrile) have been used, the aqueous component often containing phosphate (pH 2-7; Gomez et al, 2005;Okuda et al, 1996;Abou-Auda et al, 1998;Gaillard and Pepin, 1997;Campins-Falco et al, 1997;Sidhu and Charles, 1993;Farthing et al, 1992;Takamura et al, 1997) or acetate buffers (pH 5-7; Jankowski et al, 1997;Nava-Ocampo et al, 1999;Abdel-Hamid, 2000). Detection is generally achieved by absorption or fluorescence spectrometry.…”

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confidence: 99%

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High‐throughput liquid‐chromatography method with fluorescence detection for reciprocal determination of furosemide or norfloxacin in human plasma

Galaon¹,

Udrescu²,

Sora³

et al. 2006

Biomedical Chromatography

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A simple, high-throughput, highly selective and sensitive HPLC-FLD method for isolation and determination of furosemide and/or norfloxacin in human plasma samples following a simple organic solvent deproteinization step with acetonitrile as sample 'clean-up' procedure is reported. One of the two drug substances plays the internal standard role for the determination of the other. Separation of analyte and internal standard was achieved in less than 5.3 min (injection to injection) on a Chromolith Performance RP-18e column, using an aqueous component containing 0.015 mol/L sodium heptane-sulfonate and 0.2% triethylamine brought to pH = 2.5 with H(3)PO(4). The composition of the mobile phase was: acetonitrile-methanol-aqueous component = 70:15:15 (v/v/v) and the flow-rate was set up to 3 mL/min. The chromatographic method applied to the determination of furosemide relies on fluorescent detection parameters of 235 nm for the excitation wavelength, and 402 nm for the emission wavelength. In case of norfloxacin, the excitation wavelength is set up to 268 nm and the emission wavelength is set up to 445 nm. The overall method leads to quantitation limits of about 27 ng/mL for furosemide, and 19.5 ng/mL for norfloxacin, using an injection volume of 250 microL. The method was applied to the bioequivalence study of two furosemide-containing formulations.

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“…Also, liquidliquid extraction and high-performance liquid chromatography were used for estimation FSD [17,18]. Spectrophotometric methods adopted different reactions for the determination of FSD in pharmaceutical dosage forms [19][20][21][22], finally, reverse-phase high-performance liquid chromatography [23], and flow injection with HPLC [24], as well as HPLC method were used in the estimation of FSD [25][26][27].…”

Section: Introductionmentioning

confidence: 99%

Visible Quantitative Methods for the Estimation of Furosemide in Pure form and Pharmaceutical Formulations

Saleem

Hamdon

Majeed

2021

JPRI

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Aims: Design of technical methods for the determination of Furosemide in its pure and pharmaceutical dosage form using spectral methods. Study Design: planned and executed to estimate Furosemide by using Visible spectrophotometric in pure and pharmaceutical dosage form. Place and Duration of Study: Laboratory of Analytical Research, chemistry department, college of Science, University of Mosul ,Mosul-Iraq, during the period of April 2021 to August 2021. Methodology: Furosemide, the commercially known drug Lazix, which is important in the treatment of heart diseases and high blood pressure. This study was carried out using JASCO V – 630, double-beam computerized UV-Visible spectrophotometer, with 1 cm matched cell, and HANA pH meter was used for reported pH readings. Results: The reaction between Furosemide and bromo-phenol blue, xylenol orange, and chromazorol S. The decreasing in the intensity of the resulted colored complex was measured using bromo-phenol blue, xylenol orange, While the increasing of the color intensity was measured in the method (C). These three methods were based on charge transfer reaction. The limits of Beer's law for method (A) 0.4-32µg. mL-1, method (B) 1-32 and method (C) were 0.8-32 depending on the level of concentration, while the values of the molar absorption coefficient 1.4×104, 2.1×104 and 1.57×104 l.mol-1.cm-1 for the first, second and third method respectively. Sandel's significance also was calculated for these three methods, 0.0157 μg.cm-2 for the first method, 0.0236 μg.cm-2 for the second method, while the third method was 0.0210 μg.cm-2. The method has been successfully applied for the determination of furosemide in its pure form and in some of its pharmaceutical preparations Conclusion: The proposed methods were validated in terms of linearity, range, Accuracy, precision, Specificity, Robustness. The proposed methods were successfully applied to the estimation of Furosemide in pharmaceutical dosage form, method (B) was experimentally considered as a best method depending on the best values of molar absorptivity, stability of the resulted complex, and the linearity of the method (B) .

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Method Validation for the Determination of Furosemide in Plasma by Liquid- Liquid Extraction and High-Performance Liquid Chromatography With Fluorescence Detection

Cited by 8 publications

References 0 publications

Analytical issues in HPLC/MS/MS simultaneous assay of furosemide, spironolactone and canrenone in human plasma samples

Analytical issues in HPLC/MS/MS simultaneous assay of furosemide, spironolactone and canrenone in human plasma samples

High‐throughput liquid‐chromatography method with fluorescence detection for reciprocal determination of furosemide or norfloxacin in human plasma

Visible Quantitative Methods for the Estimation of Furosemide in Pure form and Pharmaceutical Formulations

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