Méthode simple d’échange de germoplasme de cocotier (<i>Cocos nucifera</i> L.) par l’utilisation d’embryons zygotiques.

Molecular diagnosis of phytoplasma transmission from zygotic embryos to in vitro regenerated plants of coconut palm (Cocos nucifera L.)

“…These nuts that do not have dormancy often carry disease germs like lethal yellowing disease (LYD). Studies on the conservation and transfer of coconut material in the form of embryos have been investigated in several laboratories (Assy-Bah et al, 1989;Danso et al, 2009;Rillo and Paloma, 1991;Cueto et al, 2012;Yoboue et al, 2014).…”

“…A total of 150 nuts were harvested from the 30 sampled mother trees. The cylinders of the endosperm or solid albumen that contain the zygotic embryos were carefully removed from the nuts and disinfected as recommended by N'Nan et al 2012and Yoboue et al (2014). After disinfection of the endosperm cylinders, extraction and disinfection of the zygotic embryos were carried out in the laboratory under aseptic conditions for operation in air-flow cabinet (ASSY-Bah et al, 1989;Yoboue et al, 2014).…”

“…The cylinders of the endosperm or solid albumen that contain the zygotic embryos were carefully removed from the nuts and disinfected as recommended by N'Nan et al 2012and Yoboue et al (2014). After disinfection of the endosperm cylinders, extraction and disinfection of the zygotic embryos were carried out in the laboratory under aseptic conditions for operation in air-flow cabinet (ASSY-Bah et al, 1989;Yoboue et al, 2014). The samplings were taken on seemingly healthy trees or in stage 1 trees because during the evolution of the disease, the mature and immature nuts fall early enough; therefore, for these stages (2, 3 and 4), there are no mature nuts on the trees.…”