Methodology, biology and clinical applications of mesenchymal stem cells

Camassola, Melissa; Braga, Luisa Maria Gomes de Macedo; Chagastelles, Pedro César; Nardi, Nance Beyer

doi:10.2741/3528

Cited by 139 publications

(61 citation statements)

References 142 publications

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“…Among the various stem cell populations used for cell therapy, adult mesenchymal stem cells (MSC, also referred to as mesenchymal stromal cells) have emerged as a major new cell technology with a diverse spectrum of potential clinical applications. 1,2 At least 92 clinical trials are currently using MSC worldwide (http://clinicaltrials.gov/).…”

Section: Introductionmentioning

confidence: 99%

In vivo MR detection of fluorine-labeled human MSC using the bSSFP sequence

Ribot¹,

Gaudet²,

Chen³

et al. 2014

IJN

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Mesenchymal stem cells (MSC) are used to restore deteriorated cell environments. There is a need to specifically track these cells following transplantation in order to evaluate different methods of implantation, to follow their migration within the body, and to quantify their accumulation at the target. Cellular magnetic resonance imaging (MRI) using fluorine-based nanoemulsions is a great means to detect these transplanted cells in vivo because of the high specificity for fluorine detection and the capability for precise quantification. This technique, however, has low sensitivity, necessitating improvement in MR sequences. To counteract this issue, the balanced steady-state free precession (bSSFP) imaging sequence can be of great interest due to the high signal-to-noise ratio (SNR). Furthermore, it can be applied to obtain 3D images within short acquisition times. In this paper, bSSFP provided accurate quantification of samples of the perfluorocarbon Cell Sense-labeled cells in vitro. Cell Sense was internalized by human MSC (hMSC) without adverse alterations in cell viability or differentiation into adipocytes/osteocytes. The bSSFP sequence was applied in vivo to track and quantify the signals from both Cell Sense-labeled and iron-labeled hMSC after intramuscular implantation. The fluorine signal was observed to decrease faster and more significantly than the volume of iron-associated voids, which points to the advantage of quantifying the fluorine signal and the complexity of quantifying signal loss due to iron.

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Section: Introductionmentioning

confidence: 99%

In vivo MR detection of fluorine-labeled human MSC using the bSSFP sequence

Ribot¹,

Gaudet²,

Chen³

et al. 2014

IJN

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“…Along with these phenotypic characteristics, MSCs secrete various anti-apoptotic, immunomodulatory, angiogenic, antiscarring, and chemoattractant bioactive molecules, providing a basis for their use as tools to create local regenerative environments in vivo. 42 Experimental and clinical data also demonstrated an immunoregulatory function of BM-derived MSC, which may contribute to the reduction of the incidence of graft versus host disease following hematopoietic stem cell transplantation.…”

Section: Hematopoietic Stem Cellsmentioning

confidence: 99%

Use of Stem Cells for Liver Diseases—Current Scenario

Kumar¹,

Pati²,

Sarin³

2011

Journal of Clinical and Experimental Hepatology

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“…We have also suggested that the perivascular location of MSCs, associated to their remarkable immunoregulatory capacity, implies a role in the maintenance of tissue homeostasis: in the case of tissue injury, MSCs secrete a panel of cytokines and factors that control the immune response to avoid an autoimmune process [8]. These studies have shown that, although similar in general characteristics, MSCs isolated from different tissues, such as brain, spleen, liver, kidney, lung, bone marrow, muscle, thymus, and pancreas, exhibit particular biological features, raising the question on whether they are identical cell populations or have important differences at the molecular level [9].…”

Section: Introductionmentioning

confidence: 99%

Molecular Analysis of the Differentiation Potential of Murine Mesenchymal Stem Cells from Tissues of Endodermal or Mesodermal Origin

Nora

Camassola²,

Bellagamba³

et al. 2012

Stem Cells and Development

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Mesenchymal stem cells (MSCs) have received great attention due to their remarkable regenerative, angiogenic, antiapoptotic, and immunosuppressive properties. Although conventionally isolated from the bone marrow, they are known to exist in all tissues and organs, raising the question on whether they are identical cell populations or have important differences at the molecular level. To better understand the relationship between MSCs residing in different tissues, we analyzed the expression of genes related to pluripotency (SOX2 and OCT-4) and to adipogenic (C/EBP and ADIPOR1), osteogenic (OMD and ALP), and chondrogenic (COL10A1 and TRPV4) differentiation in cultures derived from murine endodermal (lung) and mesodermal (adipose) tissue maintained in different conditions. MSCs were isolated from lungs (L-MSCs) and inguinal adipose tissue (AMSCs) and cultured in normal conditions, in overconfluence or in inductive medium for osteogenic, adipogenic, or chondrogenic differentiation. Cultures were characterized for morphology, immunophenotype, and by quantitative real-time reverse transcription-polymerase chain reaction for expression of pluripotency genes or markers of differentiation. Bone marrow-derived MSCs were also analyzed for comparison of these parameters. L-MSCs and A-MSCs exhibited the typical morphology, immunophenotype, and proliferation and differentiation pattern of MSCs. The analysis of gene expression showed a higher potential of adipose tissue-derived MSCs toward the osteogenic pathway and of lung-derived MSCs to chondrogenic differentiation, representing an important contribution for the definition of the type of cell to be used in clinical trials of cell therapy and tissue engineering.

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Methodology, biology and clinical applications of mesenchymal stem cells

Cited by 139 publications

References 142 publications

In vivo MR detection of fluorine-labeled human MSC using the bSSFP sequence

In vivo MR detection of fluorine-labeled human MSC using the bSSFP sequence

Use of Stem Cells for Liver Diseases—Current Scenario

Molecular Analysis of the Differentiation Potential of Murine Mesenchymal Stem Cells from Tissues of Endodermal or Mesodermal Origin

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