Methodology for the selection and isolation of phenol degrading microorganisms

Bastos, Artur Eduardo Ribeiro; Moon, David Henry; Furlan, Gabriel; Tornisiello, V.; Tsai, Shih Ming

doi:10.1016/0964-8305(96)84348-4

Cited by 5 publications

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…(PN1001) that actively utilized and degraded pentylaniline and aniline as carbon source with no glucose/starch degradation has being reported by Wang et al (2006). Transformation processes are accelerated by adaptation to aromatic compounds which are basic criterion for microbial selections (Bastos et al, 1995). Shake flask transformation of aniline was studied and selection of ST1 was done by adapting it to higher concentration (2000 ppm) of aniline with and without glucose.…”

Section: Discussionmentioning

confidence: 99%

Isolation and characterization of a bacterial strain for aniline degradation

Ahmed¹,

Ahmed²,

Nisar³

et al. 2010

Afr. J. Biotechnol.

View full text Add to dashboard Cite

Aniline, a serious environmental threat and health risk to living organisms is being released into the soil and water bodies owing to its expanded use in industry. The objective of the present study was to isolate a strain from rhizospheric soil samples of wheat (Triticum aestivum L.) taken from an agricultural site near the industrial area of Faisalabad, with the capability of degrading aniline with its maximum activity. The isolated strain was identified as Staphylococcus aureus ST1 a newly reported strain for aniline degradation. The strain ST1 showed tolerance up to 2000 ppm for aniline on mineral salt media plates and its degradative ability was checked through shake flasks experiments using HPLC. The strain was capable of degrading aniline and utilizing it as a sole source of carbon and energy. Maximum reduction of aniline concentration in medium up to 59.65% was observed after 72 h. An enhancement in biodegradation was observed using glucose as an additional growth substrate. The degradative products analyzed by HPLC were catechol, phenol and some other unknown compounds. Plasmid curing showed the involvement of plasmid encoded genes which was later followed by the isolation of plasmid DNA, which was found to be a large one of ~40 kb having restriction sites for enzymes (EcoRI, BamHI, ClaI, StuI, PstI, and HindIII) used.

show abstract

Section: Discussionmentioning

confidence: 99%