Methods and Protocols of Modern Solid Phase Peptide Synthesis

Amblard, Muriel; Fehrentz, Jean‐Alain; Martinez, Jean; Subra, Gilles

doi:10.1385/mb:33:3:239

Cited by 432 publications

(344 citation statements)

References 8 publications

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“…It was the fact that reliable quantification of remaining functional groups on particles is rather difficult, stimulated solid phase peptide synthesizers to develop highly efficient coupling strategies [313]. These strategies will be further discussed in the next section.…”

Section: Chemical Analysismentioning

confidence: 99%

Porous polymer particles—A comprehensive guide to synthesis, characterization, functionalization and applications

Gökmen

Prez

2012

Progress in Polymer Science

451

309

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Section: Chemical Analysismentioning

confidence: 99%

Porous polymer particles—A comprehensive guide to synthesis, characterization, functionalization and applications

Gökmen

Prez

2012

Progress in Polymer Science

451

309

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“…TRP3 was synthesized manually using solid-phase methodology (36) with fluorenylmethyloxycarbonyl chloride (Fmoc)-protected amino acids; the synthesis was performed as described by Amblard et al (37) and Chan and White (38). TRP3 was purified using reverse-phase high-performance liquid chromatography (HPLC) until > 95%, and the mass was checked by matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry (theoretical m/z ¼ 1901.28; found, 1901.3).…”

Section: Peptide Synthesismentioning

confidence: 99%

Structural and Dynamic Insights of the Interaction between Tritrpticin and Micelles: An NMR Study

Santos

Moraes

Nakaie

et al. 2016

Biophysical Journal

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A large number of antimicrobial peptides (AMPs) acts with high selectivity and specificity through interactions with membrane lipid components. These peptides undergo complex conformational changes in solution; upon binding to an interface, one major conformation is stabilized. Here we describe a study of the interaction between tritrpticin (TRP3), a cathelicidin AMP, and micelles of different chemical composition. The peptide's structure and dynamics were examined using one-dimensional and two-dimensional NMR. Our data showed that the interaction occurred by conformational selection and the peptide acquired similar structures in all systems studied, despite differences in detergent headgroup charge or dipole orientation. Fluorescence and paramagnetic relaxation enhancement experiments showed that the peptide is located in the interface region and is slightly more deeply inserted in 1-myristoyl-2-hydroxy-sn-glycero-3-phospho-1 0 -rac-glycerol (LMPG, anionic) than in 1-lauroyl-2-hydroxy-sn-glycero-3-phosphocholine (LLPC, zwitterionic) micelles. Moreover, the tilt angle of an assumed helical portion of the peptide is similar in both systems. In previous work we proposed that TRP3 acts by a toroidal pore mechanism. In view of the high hydrophobic core exposure, hydration, and curvature presented by micelles, the conformation of TRP3 in these systems could be related to the peptide's conformation in the toroidal pore.

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“…Rat PYY(3-36) was synthesized manually by utilizing Fmoc batch-wise solid-phase methodology [3]. Purification was accomplished by reverse phase high performance liquid chromatography.…”

Section: Synthesis and Purification Of Pyy(3-36)mentioning

confidence: 99%

PYY(3–36) induces Fos in the arcuate nucleus and in both catecholaminergic and non-catecholaminergic neurons in the nucleus tractus solitarius of rats

et al. 2008

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Peptide YY ] inhibits feeding in rodents, nonhuman primates and humans, yet the neural circuits underlying this action remain to be determined. Here we assessed whether PYY(3-36) inhibits feeding by activating neurons in forebrain and hindbrain sites containing Y2 receptors and linked to control of food intake, or in hindbrain sites immediately downstream of vagal afferent neurons. Rats received an anorexigenic dose of PYY(3-36), and the number of neurons expressing Fos, an indicator of neural activation, was determined in anterior hypothalamus (AH), arcuate nucleus (ARC), dorsomedial hypothalamus (DMH), lateral hypothalamus (LH), ventromedial hypothalamus (VMH), central nucleus of the amygdala (CeA), area postrema (AP), and caudal medial nucleus tractus solitarius (cmNTS), commissural NTS (cNTS), and gelatinosus NTS (gNTS). Expression of tyrosine hydroxylase (TH), an indicator of catecholamine synthesis, was also measured in the cmNTS. PYY(3-36) increased Fos in ARC, cmNTS, gNTS and AP. Approximately 10% of Fos (+) neurons in the cmNTS were TH (+). These results suggest that PYY(3-36) inhibits feeding through direct activation of ARC neurons, and direct and/or indirect activation via vagal afferent nerves of cmNTS, gNTS and AP, including some catecholaminergic neurons in the cmNTS.

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Methods and Protocols of Modern Solid Phase Peptide Synthesis

Cited by 432 publications

References 8 publications

Porous polymer particles—A comprehensive guide to synthesis, characterization, functionalization and applications

Porous polymer particles—A comprehensive guide to synthesis, characterization, functionalization and applications

Structural and Dynamic Insights of the Interaction between Tritrpticin and Micelles: An NMR Study

PYY(3–36) induces Fos in the arcuate nucleus and in both catecholaminergic and non-catecholaminergic neurons in the nucleus tractus solitarius of rats

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