Methods for assessing the composition and diversity of soil microbial communities

Hill, G. T.; Mitkowski, N. A.; Aldrich‐Wolfe, Laura; Emele, L.R.; Jurkonie, D.D.; Ficke, Andrea; Maldonado-Ramírez, Sandra L.; Lynch, S.; Nelson, Eric B.

doi:10.1016/s0929-1393(00)00069-x

Cited by 412 publications

(241 citation statements)

References 97 publications

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“…play an important role in soil process that determines plant productivity. For successful functioning of introducing microbial bioinoculants and their infl uence on health, exhaustive efforts have been made to explore soil microbial diversity of indigenous community, their distribution and behavior in soil habitats [12].…”

Section: Generic Composition In Water Samplementioning

confidence: 99%

Microbial diversity in firework chemical exposed soil and water samples collected in Virudhunagar district, Tamil Nadu, India

Dhasarathan¹,

Theriappan²,

Ashokraja³

2009

Indian J Microbiol

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Microbial diversity of soil and water samples collected from pyrochemicals exposed areas of Virdhunagar district (Tamil Nadu, India) was studied. Soil and water samples from cultivable area, waste land and city area of the same region were also studied for a comparative acount. There is a remarkable reduction in total heterotrophic bacterial population (THB) in pyrochemicals exposed soil and water samples (42 × 10 4 CFU/g and 5.6 × 10 4 CFU/ml respectively), compared to the THB of cultivable area soil and water samples (98 × 10 7 CFU/g and 38.6 × 10 7 CFU/ml). The generic composition the THB of the pyrochemicals exposed samples too exhibited considerable change compared to other samples. Pseudomonas sp. was the predominant one (41.6%) followed by Achromobacter sp. (25%) in pyrochemical exposed soil and Pseudomonas sp. was the predominant one (25%) in pyrochemical exposed water samples followed by Bacillus sp. (25%) and Micrococcus sp. (16.6%). It was observed that Cornybacterium sp. and Micrococcus sp. were absent completely in pyrochemical exposed soil and Achromobacter sp. was missing in the pyrochemical exposed water samples, which were present in the other samples. The outcome of this study clearly demonstrates that pollutants such as chemicals used in pyrotechniques affect the microbial biodiversity and suitable measures have to be taken to control the pollution level and to save biodiversity.

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Section: Generic Composition In Water Samplementioning

confidence: 99%

Microbial diversity in firework chemical exposed soil and water samples collected in Virudhunagar district, Tamil Nadu, India

Dhasarathan¹,

Theriappan²,

Ashokraja³

2009

Indian J Microbiol

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“…Fatty acid Hill et al (2000) 3.2.2. Effect of solubilization treatment on rhizospheric enzymes EPS is a complex high-molecular polymer that hinders nutrient transfer to microbes in EPS and binds to enzymes after accumulating excessively.…”

Section: Groupmentioning

confidence: 99%

EPS solubilization treatment by applying the biosurfactant rhamnolipid to reduce clogging in constructed wetlands

Trinh

et al. 2016

Bioresource Technology

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“…Microbial communities are critical to soil function (Mummey, Stahl & Buyer, 2002) and are responsive to small changes due to soil ecological stress or restoration processes (Badiane et al, 2002). Assessment of soil microbial properties and microbial diversity can therefore be used as sensitive indicators of both short and long-term changes (Hill et al, 2000) that occur in the soil environment. Studies in agricultural environments have shown that enzymatic activities give an indication of the diversity of functions that can be assumed by the microbial community (Bandick & Dick, 1999;Aon & Colaneri, 2001;Brohon, Delolme & Gourdon, 2001).…”

Section: Introductionmentioning

confidence: 99%

“…Studies in agricultural environments have shown that enzymatic activities give an indication of the diversity of functions that can be assumed by the microbial community (Bandick & Dick, 1999;Aon & Colaneri, 2001;Brohon, Delolme & Gourdon, 2001). The use of a culture-independent method (Hill et al, 2000), such as phospholipid fatty acid (PLFA) analysis circumvents many of the problems frequently associated with conventional culture dependent techniques and provides a more comprehensive view ofthe structure of complex microbial communities (Ibekwe & Kennedy, 1998;Pinkart, Deveraux & Chapman, 1998). Recent studies suggest that PLFA profiles could be used as 'fingerprints' indicative of successful restoration of soil communities and as indicators of responses to management practices and changes in soil quality, and that changes in PLFA profiles could reflect past and present management practices (Ponder & Tadros, 2002;Steenwerth et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Microbial community function and structure on coal mine discard under rehabilitation

Claassens

Riedel

Rensburg

et al. 2006

South African Journal of Plant and Soil

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The rehabilitation of coal mine discard is problematic, especially since the lack of measures to evaluate the success of rehabilitation in terms of the self-sustainability of soil ecosystems established on these sites. In this study, the potential for using microbial community function and structure as such a measure was investigated using enzymatic assays (dehydrogenase, B-glucosidase, urease and acid and alkaline phosphatase) and signature lipid biomarkers (PLFAs). Samples from seven coal discard sites in South Africa currently under rehabilitation. were investigated and the relationship between soil properties, vegetation cover and microbial properties analysed using PCA and RDA ordination techniques. Although the discard sites had different rehabilitation ages (one to eight years). no statistically significant differences existed between these sites based on physical and chemical characteristics. Differentiation w~s possible based on enzymatic activities and PLFA profiles. Sites with relatively higher vegetation cover and organic carbon content had a positive association with enzymatic activities and microbial biomass. Organic carbon content correlated significantly with B-glucosidase (r = 0.80, P<0.05). urease (r = 0.96, P<0.05), acid phosphatase (r = 0.76, P<0.05), dehydrogenase (r = 0.69, P<0.10) and microbial biomass (r = 0.73. P<0.1 0). The characterisation of microbial community function and structure holds potential for evaluating rehabilitation progress on mine discard sites.

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Methods for assessing the composition and diversity of soil microbial communities

Cited by 412 publications

References 97 publications

Microbial diversity in firework chemical exposed soil and water samples collected in Virudhunagar district, Tamil Nadu, India

Microbial diversity in firework chemical exposed soil and water samples collected in Virudhunagar district, Tamil Nadu, India

EPS solubilization treatment by applying the biosurfactant rhamnolipid to reduce clogging in constructed wetlands

Microbial community function and structure on coal mine discard under rehabilitation

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