Methods for Estimation of Blood Glucose : A Comparative Evaluation

Ambade, Vivek; Sharma, Y V; Somani, Babu Lal

doi:10.1016/s0377-1237(17)30502-6

Cited by 57 publications

(40 citation statements)

References 9 publications

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“…19 An automated KONELAB 3i analyzer (Thermofisher Scientific, Waltham, MA, USA) with the GOD-POD and Hexokinase method as per the instructions given with the kits (Thermofisher Scientific, Waltham, MA, USA) were used to estimate blood glucose levels. 20 Briefly, serum samples were mixed with the kit's colorimetric substrate and horseradish peroxidase (Thermofisher Scientific, Waltham, MA, USA) and the reaction was initiated by addition of glucose oxidase (Sigma-Aldrich, St. Louis, Missouri, USA). The microplate was incubated at room temperature for 30 minutes.…”

Section: Determination Of Blood Glucose Levelsmentioning

confidence: 99%

Correlation of Apgar score with serum glucose, calcium and electrolytes on the asphyxiated neonates

et al. 2019

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Background: Neonatal asphyxia is characterized by discrepancy of oxygen during perinatal period that can lead to severe hypoxic ischaemic organ damages followed by a fatal outcome including neurodegenerative diseases, mental retardation, and epilepsies. According to world health organization, four million neonatal deaths occurred each year due to birth asphyxia. Therefore, our study was designed to evaluate the status of serum glucose, calcium, electrolytes, and their correlation with the fetal risk factors associated with birth asphyxia.Methods: Neonates diagnosed with birth asphyxia were considered as “cases” while neonates birth either normal or by cesarean with having no abnormality were considered as “control”. Demographics and possible risk factors of both the mother and neonate were noted. All asphyxiated neonates and controls were chosen to examine for serum glucose, calcium and electrolytes. Automated analyzers were used to estimate serum glucose, calcium, sodium and potassium. Results: We found that the mean serum glucose level was significantly lower in the asphyxiated neonates compared with controls, and consequently showed very strong positive correlation with the Apgar score. Furthermore, significant reduction levels were observed in serum calcium and sodium in the asphyxiated neonates, showing a linear correlation with the Apgar score. Moreover, higher serum potassium was detected in the asphyxiated neonates than in controls, showing a negative correlation with the Apgar score.Conclusions: We validated that the examined biochemicals of asphyxiated neonates was strongly correlated with the Apgar score. Our study reinforces for adequate clinical evaluation and biochemical monitoring for early diagnosis to prevent adverse neurodevelopmental outcome.

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Section: Determination Of Blood Glucose Levelsmentioning

confidence: 99%

Correlation of Apgar score with serum glucose, calcium and electrolytes on the asphyxiated neonates

et al. 2019

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“…The obtained results indicated a trend of improvement in total cholesterol and LDL-C levels in the control group compared to the intervention one, which could be attributed to better compliance of the participants with the given diet and physical activities in the control group. Some of the studies in this regard have shown that the prescription of medication would lead to less consideration of pharmacological recommendations among the patients [17] . As lifestyle modification was applied in both groups, it was to some extent expectable to achieve such results, since the use of this approach for controlling the daily calorie intake is considered as a gold standard of care for the patients with NAFLD [18] .…”

Section: Discussionmentioning

confidence: 99%

Effect of Vitamin D Replacement on Liver Enzymes in Patients with Non-alcoholic Fatty Liver Disease

Hajiaghamohammadi

Shafikhani

Bastani

et al. 2019

Journal of Gastroenterology and Hepatology Research

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BACKGROUND AND AIMS: The relationship between vitamin D-deficiency and pathogenesis of non-alcoholic fatty liver diseases (NAFLD) has been always a controversial subject. The present study is aimed to investigate the effects of vitamin D on the reduction of liver enzymes and, then, compare its effects with those of diet and physical activities individually. PATIENTS AND METHODS: The present double-blinded randomized clinical trial was conducted at …..University of Medical Sciences in 2017. For this purpose, 80 patients with NAFLD and vitamin D-deficiency were randomly divided into two groups, namely intervention and control. Subsequently, the patients in both groups were provided with programs of diets and physical activities. The patients in the intervention group received, in addition to the given treatment, the vitamin at the dosage of (VIT-D/50000 unit) once per week for ten weeks. Afterwards, the data related to the clinical and demographic characteristics of both groups were collected and, then, compared with each other using statistical methods. RESULTS: The applied therapeutic interventions led to a significant reduction in the variables including AST, ALT, total cholesterol, and LDL-C in both groups (p < 0.05). As for the two indices of blood sugar and triglyceride, the control group exhibited significantly less variations (p < 0.05). C O N C L U S I O N: The obtained results showed that the administration of vitamin D had no considerable superiority over the diet and physical activities in terms of reducing the liver enzymes as well as other investigated parameters.

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“…The solid residue was discarded and a filtrate of the sample was centrifuged at 5,000 rpm for 5 min. The glucose concentration was determined by GOD–POD method . The percentage of cellulose hydrolysis was calculated by using Equation :

% Cellulose hydrolysis = \frac{Glucose concentration after enzymatic hydrolysis \times 100}{Cellulose before enzymatic hydrolysis \times 1.11}

…”

Section: Methodsmentioning

confidence: 99%

“…The glucose concentration was determined by GOD-POD method. 21 The percentage of cellulose hydrolysis was calculated by using Equation (2):…”

Section: Enzymatic Hydrolysismentioning

confidence: 99%

Response surface optimization of acid pretreatment of cassava stem for bioethanol production

Sivamani¹,

Ramesh²,

Chandrasekaran

2019

Env Prog and Sustain Energy

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Agricultural waste, cassava stem was found to contain about 25 and 40% of hemicellulose and cellulose, respectively. Bioethanol is produced from cassava stem by dilute acid pretreatment followed by alkali delignification, cellulase digestion, and fermentation by Fusarium oxysporum. Dilute acid pretreatment was optimized by response surface methodology based Box–Behnken design. The optimum xylose concentration obtained by the experiment was 11.24 g/L and the model was sulfuric acid concentration of 2.02% (v/v), solid to liquid ratio of 0.05 g/ml and 79 min. After alkali delignification and cellulase hydrolysis of pretreated cassava stem, the glucose content was 18.1 g/L and the bioethanol concentration obtained through co‐fermentation of xylose and glucose by F. oxysporum was 13.62 g/L. The optimum values indicated that 91.1% yield was achieved with cassava stem using dilute sulfuric acid pretreatment. Hence, the dilute acid pretreatment of cassava stem was found to be the promising method for bioethanol production.

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Methods for Estimation of Blood Glucose : A Comparative Evaluation

Cited by 57 publications

References 9 publications

Correlation of Apgar score with serum glucose, calcium and electrolytes on the asphyxiated neonates

Correlation of Apgar score with serum glucose, calcium and electrolytes on the asphyxiated neonates

Effect of Vitamin D Replacement on Liver Enzymes in Patients with Non-alcoholic Fatty Liver Disease

Response surface optimization of acid pretreatment of cassava stem for bioethanol production

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