Methods for purifying and detoxifying sodium dodecyl sulfate–stabilized polyacrylate nanoparticles

Abstract

Recent research in our laboratory has centered on studies of polyacrylate and polyacrylamide nanoparticle emulsions for use in antibiotic delivery. Our goal is to develop these nanoparticle emulsions for treatment of life-threatening bacterial infections such as those caused by methicillinresistant Staphylococcus aureus (MRSA). For this intended application, it is necessary to ensure that the biological activity of the emulsion is due only to the drug attached to the polymeric chain, rather than to any extraneous components. To investigate this, we evaluated cytotoxicity and microbiological activity of the nanoparticle emulsions before and after purification by centrifugation, dialysis, and gel filtration. Depending on the amount of surfactant used, all or most of the microbial and cellular toxicity can be removed by a simple purification procedure.

Cannabinoid derivate-loaded PLGA nanocarriers for oral administration: formulation, characterization, and cytotoxicity studies

“…17,18 In the present work, Pluronic F68 was employed to stabilize the NPs and could be considered as a valuable alternative to poly(vinyl alcohol) and a promising FDAapproved surface active agent for clinical use. 19 To determine the residual amounts of Pluronic F68 on PLGA NPs, 1 H-NMR (500 MHz) spectra were recorded at 303 K in CDCl 3 , on a Brüker Avance 500 spectrometer (Brüker AXS Inc, Madison, WI).…”

In vivo studies of polyacrylate nanoparticle emulsions for topical and systemic applications

“…Both the drug-free (NP0) and penicillin-containing (NP1) nanoparticle emulsions were tested in mice using a skin abrasion model as well as systemically by intraperitoneal injection. 3,5,6,45 For each analysis the dosing concentration and regimens were varied, and drug-free nanoparticle samples as well as saline controls were used. The various emulsion concentrations were determined through serial dilution of the emulsions so as to establish a full range of concentrations for analysis.…”

“…Thus, our initial interest is to develop the emulsions for these two specific applications, namely, the treatment and prevention of open wound infections in the skin and systemic infections. 6,44,45 Our first objective, and the focus of this study, is to ascertain the in vivo toxicity and biocompatibility of poly(butyl acrylate-styrene) nanoparticle emulsions for these administrative routes.…”

Tailoring Confinement: Nano-Carrier Synthesis via Z-RAFT Star Polymerization

“…Polybutylacrylate (PBA) was also chosen for the present study as the hydrophobic part of the star polymer template, as it is known to easily encapsulate hydrophilic drugs [43][44][45][46]. BA polymerizes over short periods of time, due to its high kp-value [47], and was extensively investigated in Z-RAFT star polymerization in our group [21,23,24,[48][49][50].…”