Methods for reducing biosensor membrane biofouling

Wisniewski, Natalie A.; Reichert, M. Shawn

doi:10.1016/s0927-7765(99)00148-4

Cited by 490 publications

(400 citation statements)

References 125 publications

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“…Nafion has been claimed to have antifouling properties [34] owing to its ability to exclude molecules of large size (e.g., MW > 200). Figure 1 shows the responses of the SH-SAMMS ™ -Nafion electrode to 25 ppb of Cd and Pb in 0.01 M CH 3 COONa without BSA (thick line) and for 6 consecutive runs after the addition of 20 mg/L of BSA (thin line) after 3 minutes of preconcentration.…”

Section: Antifouling Properties Of Nafion In Protein Rich Samplesmentioning

confidence: 99%

Detection of Cd, Pb, and Cu in non-pretreated natural waters and urine with thiol functionalized mesoporous silica and Nafion composite electrodes

Yantasee

Charnhattakorn

Fryxell

et al. 2008

Analytica Chimica Acta

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Electrochemical sensors have great potential for environmental monitoring of toxic metal ions in waters due to their portability, field-deployability and excellent detection limits. However, electrochemical sensors employing mercury-free approaches typically suffer from binding competition for metal ions and fouling by organic substances and surfactants in natural waters, making sample pretreatments such as wet ashing necessary. In this work, we have developed mercury-free sensors by coating a composite of thiol self-assembled monolayers on mesoporous supports (SH-SAMMS ™ ) and Nafion on glassy carbon electrodes. With the combined benefit of SH-SAMMS ™ as an outstanding metal preconcentrator and Nafion as an antifouling binder, the sensors could detect 0.5 ppb of Pb and 2.5 ppb of Cd in river water, Hanford groundwater, and seawater with a minimal amount of preconcentration time (few minutes) and without any sample pretreatment. The sensor could also detect 2.5 ppb of Cd, Pb, and Cu simultaneously. The electrodes have long service times and excellent single and inter-electrode reproducibility (5% RSD after 8 consecutive measurements). Unlike SAMMS ™ -carbon paste electrodes, the SAMMS ™ -Nafion electrodes were not fouled in samples containing albumin and successfully detected Cd in human urine. Other potentially confounding factors affecting metal detection at SAMMS ™ -Nafion electrodes were studied, including pH effect, transport resistance of metal ions, and detection interference. With the ability to reliably detect low metal concentration ranges without sample pretreatment and fouling, SAMMS ™ -Nafion composite sensors have the potential to become the next generation metal analyzers for environmental and bio-monitoring of toxic metals.

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Section: Antifouling Properties Of Nafion In Protein Rich Samplesmentioning

confidence: 99%

Detection of Cd, Pb, and Cu in non-pretreated natural waters and urine with thiol functionalized mesoporous silica and Nafion composite electrodes

Yantasee

Charnhattakorn

Fryxell

et al. 2008

Analytica Chimica Acta

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“…Biofouling has been proposed as the main reason for biosensor failure in vivo [18]. It can be characterized as accumulation of undesired biological material, such as proteins and cells, on sensor surface [18], [19].…”

Section: Introductionmentioning

confidence: 99%

Electrochemical detection of hydrogen peroxide on platinum-containing tetrahedral amorphous carbon sensors and evaluation of their biofouling properties

Tujunen

Kaivosoja

Protopopova

et al. 2015

Materials Science and Engineering: C

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Hydrogen peroxide is the product of various enzymatic reactions, and is thus typically utilized as the analyte in biosensors. However, its detection with conventional materials, such as noble metals or glassy carbon, is often hindered by slow kinetics and biofouling of the electrode. In this study electrochemical properties and suitability to peroxide detection as well as ability to resist biofouling of Pt-doped ta-C samples were evaluated. Pure ta-C and pure Pt were used as references. According to the results presented here it is proposed that combining ta-C with Pt results in good electrocatalytic activity towards H2O2 oxidation with better tolerance towards aqueous environment mimicking physiological conditions compared to pure Pt. In biofouling experiments, however, both the hybrid material and Pt were almost completely blocked after immersion in protein-containing solutions and did not produce any peaks for ferrocenemethanol oxidation or reduction. On the contrary, it was still possible to obtain clear peaks for H2O2 oxidation with them after similar treatment. Moreover, quartz crystal microbalance experiment showed less protein adsorption on the hybrid sample compared to Pt which is also supported by the electrochemical biofouling experiments for H2O2 detection.

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“…Implantable glucose biosensors are dependant on the transport of analytes to and from their surface. It is now well established that upon implantation, denatured and non-native protein structures facilitate the eventual formation of a fibrous capsule, responsible for limiting the utility of the biosensor in an in vivo environment [4]. To alter the typical biological response and limit the formation of a fibrous capsule, various approaches have been taken, the majority of which has revolved around creating "stealth" or "non-fouling" surface coatings, impervious to protein adsorption.…”

Section: Introductionmentioning

confidence: 99%

Surface modification of a perfluorinated ionomer using a glow discharge deposition method to control protein adsorption

et al. 2008

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Nafion™ is the membrane material preferred for in situ glucose sensors. Unfortunately, surface properties of Nafion promote random protein adsorption and eventual foreign body encapsulation thus leading to loss if glucose signal over time. Here we detail surface modifications made by RF plasma deposition to Nafion with the intent to prevent random protein adsorption while providing enough functional sites (hydroxyl groups) to bind a biologically active peptide known to induce cellular adhesion (YRGDS). Nafion surfaces were modified by RF plasma polymerizing five different combinations of (1) tetraethylene glycol dimethyl ether (tetraglyme) and (2) 2-hydroxyethyl methacrylate (HEMA): pure tetraglyme, 2.5% HEMA/97.5% tetraglyme; 5% HEMA/95% tetraglyme, 10% HEMA/90% tetraglyme; and pure HEMA. Resultant surfaces were characterized by XPS (low and high resolution), dynamic contact angle, and atomic force microscopy. Protein adsorption and retention was determined and correlated to surface layer composition. The ability to bind a cell adhesion peptide was also determined and correlated well with surface layer composition.

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Methods for reducing biosensor membrane biofouling

Cited by 490 publications

References 125 publications

Detection of Cd, Pb, and Cu in non-pretreated natural waters and urine with thiol functionalized mesoporous silica and Nafion composite electrodes

Detection of Cd, Pb, and Cu in non-pretreated natural waters and urine with thiol functionalized mesoporous silica and Nafion composite electrodes

Electrochemical detection of hydrogen peroxide on platinum-containing tetrahedral amorphous carbon sensors and evaluation of their biofouling properties

Surface modification of a perfluorinated ionomer using a glow discharge deposition method to control protein adsorption

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