Methods for the determination of glucose in blood. Part 2

Abstract

The chronological development of destructive and nondestructive methods designed to determine the glucose concentration in plasma, serum, and whole blood is presented in the review. Destructive procedures are divided into spectrophotometric, spectrofluorimetric, diffraction, amper ometric, potentiometric, conductometric, chromatographic, gas chromatographic-mass spectromet ric, titrimetric, and calorimetric types, as well as reflectance photometry. A brief overview of these methods and the results of comparative tests are given; the procedures are systematized. It is noted that certain types of enzymatic spectrophotometric and amperometric methods have received wide accep tance for analyzes in clinical diagnostic laboratories. Outside clinical laboratories, enzymatic amper ometric and spectrophotometric procedures and reflectance photometry procedures using test strips are widely used. Chromatography-mass spectrometry procedures are often applied to certify standard serum samples. Spectrofluorimetric, diffraction, and chromatographic techniques are common in sci entific biomedical research. Nondestructive methods like nonenzymatic spectrophotometric, nonen zymatic amperometric, potentiometric, conductometric, titrimetric, and calorimetric procedures, as well as a number of enzymatic spectrophotometric procedures, in most cases, are not used in modern clinical and diagnostic practice.

Geraniol ameliorates the progression of high fat‐diet/streptozotocin‐induced type 2 diabetes mellitus in rats via regulation of caspase‐3, Bcl‐2, and Bax expression

“…The glucose level in the serum was evaluated by the colorimetric approach of Buzanovskii (2015). On the other hand, insulin was determined by a quantitative ELISA kit based on the method of Matthews et al (1985).…”

Actinidia deliciosa Extract as a Promising Supplemental Agent for Hepatic and Renal Complication-Associated Type 2 Diabetes (In Vivo and In Silico-Based Studies)

“…Fasting glucose was evaluated in serum by a colorimetric method according to Buzanovskii [ 37 ]. Additionally, the HbA1c was assessed by standard colorimetric kits (BioAssay Systems, Hayward, CA, USA) according to the manufacturer’s guidelines.…”

Enrichment of Grain Hydrolysate With Bacterial Protein

“…The qualitative determination of starch was carried out with a starch iodine starch test under standard conditions (+ 37°C, 10 min) [1]. The glucose content in the samples was performed using the glucose oxidase method [2] using the commercial kit "Vital". The content of lactic acid in the samples was determined by the lactate oxidase method [3] also using the commercial kit "Vital".…”