Methods for the estimation of high density cholesterol, comparison between two laboratories

Haar, Frits van der; Gent, C.M. van; Schouten, F M; Voort, H.A. van der

doi:10.1016/0009-8981(78)90282-6

Cited by 29 publications

(6 citation statements)

References 14 publications

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“…Free fatty acids, total cholesterol, triglyceride and phospholipid were measured using an enzymatic colorimetric method [9,10,11,12]. High density lipoprotein was measured using a direct method [13]. Low density lipoproteins, very low density lipoproteins and chylomicrons were measured using heparin‐calcium precipitation methods [14] using instruments [TBA 80FR NEO or TBA 80M (Toshiba Medical Systems, Tokyo, Japan)] in the chemical laboratory attached to our Institute.…”

Section: Methodsmentioning

confidence: 99%

Propofol as a continuous infusion during cardiopulmonary bypass does not affect changes in serum free fatty acids

et al. 2001

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Perioperative myocardial infarction or ischaemia is a potential consequence of cardiac surgery and elevated free fatty acids can increase the severity of myocardial ischaemic damage. We investigated perioperative changes in serum free fatty acids, and other serum lipids, as a consequence of using propofol infusions for cardiac surgery during cardiopulmonary bypass. Twenty-five patients undergoing elective coronary artery bypass grafting were allocated to two groups. One group of 12 patients was given a continuous infusion of propofol and the other group of nine patients received intermittent boluses of midazolam as a hypnotic agent. Serum lipid concentrations were measured at four periods perioperatively. Changes in free fatty acid concentrations were similar between the two groups. Lipid concentrations related to triglyceride in the propofol group decreased on one occasion but subsequently returned to control value. On the other hand, such values in the midazolam group remained lower than control values. Propofol is not a contraindication as an anaesthetic for cardiac surgery in respect of concern regarding the effects of free fatty acids.

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Section: Methodsmentioning

confidence: 99%

Propofol as a continuous infusion during cardiopulmonary bypass does not affect changes in serum free fatty acids

et al. 2001

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“…Total cholesterol and HDL cholesterol were measured by enzymatic methods. 52,53 TGs were measured by an enzymatic method based on the hydrolysis of TGs by lipase to glycerol and free fatty acids. Low-density lipoprotein (LDL) cholesterol was calculated using the Friedewald equation (LDL-cholesterol=Total choles-terolÀHDL cholesterolÀTGs).…”

Section: Measurement Of Crp Glucose and Lipidsmentioning

confidence: 99%

Gender‐Specific Risk Factors for Peripheral Artery Disease in a Voluntary Screening Population

Hiramoto

Katz

Weisman

et al. 2014

JAHA

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BackgroundWomen have high rates of peripheral artery disease (PAD) despite fewer cardiovascular disease (CVD) risk factors, compared to men. We sought to determine the gender‐specific prevalence of low ankle brachial index (ABI) and the relationship to C‐reactive protein (CRP) levels and CVD risk factors in the Life Line Screening population.Methods and ResultsBetween April 2005 and August 2011, 133 750 women and 71 996 men had ABI and CRP measured at a Life Line Screening Center. Women were slightly older than men, whereas men were more likely to be current smokers, have diabetes mellitus (DM), and coronary artery disease (CAD) (P<0.001 for each). Women were more likely to have ABI≤1.0, compared to men (26.6% versus 14.4%, respectively; P<0.001), as well as ABI≤0.9 (4.1% women versus 2.6% men; P<0.001). Women had higher median CRP levels (1.94 mg/L; interquartile range [IQR], 0.89, 4.44 mg/L), compared to men (1.35 mg/L; IQR, 0.73, 2.80 mg/L; P<0.001). Men and women shared similar risk factors for ABI≤0.9, including older age, black race, smoking, DM, hypertension, hypercholesterolemia, CAD, and elevated CRP levels. In an adjusted model, there were significant interactions between gender and age (P<0.001), CRP (P<0.001), CAD (P=0.03), and DM (P=0.06) with ABI as the outcome. The associations between age, CRP, CAD, and DM with ABI≤0.9 were stronger in men than in women.ConclusionsWomen participating in the Life Line Screening had higher CRP levels and a higher prevalence of PAD, compared to men. Neither higher CRP levels nor conventional CVD risk factors explained the excess prevalence of PAD in women.

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“…Total and HDL cholesterol were determined enzymatically with the CHOD-PAP monotestkit from Boehringer Mannheim GmbH, Mannheim, Germany (19,20). Standardization was realized by using calibration sera from the Foundation of Chemical Analysis Quality Control (the Netherlands) for total cholesterol, and calibration sera were prepared according to the method of van der Haar et al (21) for HDL cholesterol. Control sera were obtained from the Centers for Disease Control and Prevention, Atlanta, Georgia.…”

Section: Examinationsmentioning

confidence: 99%

Total and High Density Lipoprotein Cholesterol as Risk Factors for Coronary Heart Disease in Elderly Men during 5 Years of Follow-up: The Zutphen Elderly Study

Weijenberg

Feskens

Kromhout

1996

American Journal of Epidemiology

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The associations of serum total and high density lipoprotein (HDL) cholesterol with coronary heart disease were investigated in men aged 64-84 years from the Dutch town of Zutphen during 5 years of follow-up. In 1985, 885 randomly selected men, 710 of whom did not have a history of clinical coronary heart disease, participated in the study. Associations were adjusted for age, body mass index, systolic blood pressure, cigarette smoking, and alcohol consumption. Total cholesterol was not significantly associated with the incidence of coronary heart disease, but for mortality the relative risk corresponding to a 1.00 mmol/liter increase was 1.40 (95 percent confidence interval (CI) 1.07-1.83). HDL cholesterol was not associated with mortality from coronary heart disease. The relative risk for the incidence of the disease, corresponding to a 0.26 mmol/liter increase, was 0.80 (95 percent CI 0.60-1.08). For the ratio of HDL cholesterol to total cholesterol, the relative risk for coronary heart disease incidence corresponding to a 0.05 increase amounted to 0.70 (95 percent CI 0.51-0.95). These results show that in elderly men followed for 5 years, both total and HDL cholesterol are important in predicting coronary heart disease. Total cholesterol seems to be a stronger risk factor for mortality from the disease, whereas HDL cholesterol is more strongly associated with the incidence of a first coronary heart disease event.

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Methods for the estimation of high density cholesterol, comparison between two laboratories

Cited by 29 publications

References 14 publications

Propofol as a continuous infusion during cardiopulmonary bypass does not affect changes in serum free fatty acids

Propofol as a continuous infusion during cardiopulmonary bypass does not affect changes in serum free fatty acids

Gender‐Specific Risk Factors for Peripheral Artery Disease in a Voluntary Screening Population

Total and High Density Lipoprotein Cholesterol as Risk Factors for Coronary Heart Disease in Elderly Men during 5 Years of Follow-up: The Zutphen Elderly Study

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