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The concentration of 5 different proteins in suction blister fluid and serum was determined by immunotechniques. These proteins, varying in size and molecular weight (6,600-2,300,000) were insulin, albumin, high density lipoprotein determined as apoprotein A-I, alpha 2-macroglobulin and low density lipoprotein measured as apoprotein B. The difference in the blister fluid/serum concentration ratio of the proteins was dependent on the molecular weight and followed mainly the law of diffusion. Moreover, the amounts of insulin, albumin and apoproteins A-I and B in suction blister fluid were the same as those reported in peripheral lymph. The results indicate that the sieve function of the capillary basement membrane remains intact during the formation of the suction blisters. Suction blister fluid might therefore be regarded as representative of interstitial fluid. The concentrations of 4 different lipids (cholesterol, cholesterolesters, triglycerides and phospholipids) were also determined and their blister fluid/serum concentration ratio proved to have a fairly constant value of 0.25.

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Preparation of an 85% to3 fatty acid concentrate from cod liver oil is described. The urea inclusion compound method was used. On the average, 265 g of concentrate was obtained from 1,000 g of cod liver oil. For stabilization of the fatty acids, 0.01% octyl gallate was added.

A cross-over study was done with 19 male and 23 female volunteers living in a monastery and a convent, respectively. The effect of a fat gish (mackerel) diet on the blood serum lipid composition was studied. As the normal diet of these volunteers was of the lacto-ovo-vegetarian type, a control diet in which the fish was replaced by full-fat cheese was used. Subjects consuming the fish diet had a daily uptake of polyunsaturated acids of the omega3 family of about 8 g; comparable amounts of linoleic acid were ingested with both diets. Both diets were consumed for a period of 3 weeks. Serum cholesterol was slightly but significantly (7.5%) lower and serum triglycerides considerably lower (35%) on the fish diet, whereas high density lipoprotein cholesterol increased slightly. Lipoprotein analysis showed a strong very low density lipoprotein decrease by the fish diet while, in the men, both low and high density lipoproteins increased. The fatty acid composition of serum lipids showed considerable differences; C20:5omega3 increased in all fractions and C22:6omega3 was found in the triglycerides and the phospholipids, but not in the sterol esters. These increases occurred chiefly at the expense of C18:1omega9 and, in particular, C18:2omega6, which indicates a replacement of omega6 by omega3 acids. Long-chain monoenoic acids which are abundant in the mackerel were not detected in any serum lipid fraction.