Methods for the synthesis of tritium-labelled fatty acids and their derivatives, oxylipins and steroids

Abstract

A conference on the physics of polymers arranged by The Institute of Physics and The Physical Society was held at The H.H. Wills Physics Laboratory of the University of Bristol OE

Synthesis of tritium labelled arachidonic acid amide and ester derivatives with dopamine, serotonin, vanillylamine, and ethyleneglycol moieties

“…The starting compound for the synthesis} [5,6,8,9,11,12,14,15-3 H 8 ] arachidonic acid -was prepared by selective hydrogenation of 5,8,11,14-eicosatetraynoic acid with gaseous tritium over Lindlar catalyst according to a procedure described earlier for the corresponding methyl ester. 11 Although direct tritiation of free eicosatetraynoic acid to labelled arachidonic acid proceeds with a lower yield than in the case of its methyl ester, the approach utilizes an acetylenic precursor stored in the carboxylic form, thus avoiding two additional steps: methylation of the starting compound and subsequent saponification of the hydrogenation product.…”

“…Catalysts, solvents and other reagents were purchased from Fluka (Buchs, Switzerland). Preparative purification of the labelled compounds was performed using a Gilson high-performance liquid Synthesis of labelled arachidonic acid: This was performed as described, 11 with minor modifications. The solution of 5,8,11,14eicosatetraynoic acid in dioxane was exposed to gaseous tritium in the presence of Lindlar catalyst (acetylenic acid-catalyst-quinoline ratio 1:1:2, mg/mg/m1) for 2 h at room temperature, the pressure of gaseous tritium was 400 hPa.…”

The efficiency of solvent‐free catalyst systems in the synthesis of tritium‐labelled biologically active compounds

“…Tritium can be introduced into organic compounds through isotopic exchange using liquid‐phase, solid‐phase, or tritiated water exchange methods. Liquid‐phase methods are carried out in the presence of the catalyst in solutions of the substance precursor to be labelled, and tritium gas as isotopic source 3, 40–44…”

5-Substituted pyridylisoxazoles as effective inhibitors of platelet aggregation

“…69 A tube containing 3 (3 pyridyl) 5 phenylisoxazole (19) (4 mg), the 5% Pd/BaSO 4 catalyst (12 mg), and 0.2 mL of dioxane was frozen with liquid nitrogen, evacuated, and filled with tritium gas up to a pressure of 350 GPa. After thawing, the content of the tube was stirred for 1 h at 25 C.…”