Methods of Characterization of Dendrimers

Targeted uptake of folic acid-functionalized iron oxide nanoparticles by ovarian cancer cells in the presence but not in the absence of serum

“…Nuclear magnetic resonance (NMR) is one of the most frequently used techniques for the analysis of small molecules, polymers and dendrimers, including 1 H NMR and 13 C NMR. 165 Briefly, experiments were performed on a Bruker Avance 400 MHz NMR instrument. 1 H NMR spectra were acquired with a spectral window of 20 ppm, an acquisition time of 4 seconds, and a relaxation delay of 1 second.…”

Characterization of polyamidoamino (PAMAM) dendrimers using in-line reversed phase LC electrospray ionization mass spectrometry

“…Many analytical chemistry tools have been used to evaluate dendrimers, but by far the most common technique is 1H NMR 14–17 . Direct analysis of PAMAM dendrimers by MALDI 6, 18–22 and ESI 2, 23 has also been reported.…”

“…13 Many analytical chemistry tools have been used to evaluate dendrimers, but by far the most common technique is 1 H NMR. [14][15][16][17] Direct analysis of PAMAM dendrimers by MALDI 6,[18][19][20][21][22] and ESI 2,23 has also been reported. While these techniques are useful they are not sufficient for evaluating the purity, stability and detecting individual components of complex dendrimer mixtures.…”