Methods of lipid‐normalization for multi‐tissue stable isotope analyses in tropical tuna

Abstract

Models using published estimates for parameters from other species cannot be used. Based on a range of lipid content that do not exceed 45%, we recommend the linear model to correct the bulk δ(13)C values in the investigated tissues but the parameters have to be estimated from a proportion of the original data for which chemical extraction is required and the isotopic values of bulk and lipid-free tissues are measured.

Effects of euthanasia methods on stable carbon (δ13C value) and nitrogen (δ15N value) isotopic compositions of fry and juvenile rainbow trout Oncorhynchus mykiss

“…The C and N contents of juvenile rainbow trout differed according to the tissue considered (F = 62.8 and 29.1, respectively, p < 0.001, Figure ; Tables S2 and S3, supporting information), a result consistent with literature data regarding the rainbow trout O. mykiss . The C content in muscle, liver, red blood cells and plasma ranged between 40% and 50% and from 30% to 40% in external tegument (including mucus and scales) and pectoral fin.…”

Energy allocation strategy of skipjack tuna Katsuwonus pelamis during their reproductive cycle

“…Furthermore, the absence of significant fluctuations in lipid concentrations in this tissue over the reproductive cycle suggests that white muscle only plays a minor role in the supply of energy for gonad maturation. As a highly migratory species, the muscle metabolism enables for burst swimming (Korsmeyer & Dewar, 2001;Sardenne et al, 2015).…”

Lipid extraction techniques for stable isotope analysis of bird eggs: Chloroform–methanol leads to more enriched 13C values than extraction via petroleum ether

“…1D Elliott et al, 2014), and may also explain discrepancies among the different equations present in the literature Yurkowski et al, 2015). Sardenne et al (2015) found little correlation between δ 13 C values in tuna muscle lipid-extracted using dichloromethane and δ 13 C estimated using arithmetic correction factors derived from experiments using chloroform-methanol, and concluded that "published estimates from other species cannot be used". The difference may be partially due to variation in solvent rather than species-specific differences.…”