Methods of refrigerated transport of corneas for transplantation

Madden, Peter; Maguire, S.H.

doi:10.1111/j.1442-9071.1996.tb00984.x

Cited by 3 publications

(3 citation statements)

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“…However, the conditions of the tissue during transportation have not been standardized, even though reevaluation of the corneas after transportation is not common [3][4][5]. An uncontrolled change of temperature can occur during transportation and may exert an unphysiological influence on the corneal endothelial cells that is potentially harmful [2,6]. In order to prevent corneal damage procedural guidelines for cornea banks recommend that transportation of organ cultured corneoscleral buttons should be performed at temperatures between 10 and 40°C [7].…”

Section: Introductionmentioning

confidence: 99%

Impact of temporary hyperthermia on corneal endothelial cell survival during organ culture preservation

Schroeter¹,

Ruggeri

Thieme

et al. 2015

Graefes Arch Clin Exp Ophthalmol

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To evaluate temporary exposure to hyperthermia for its impact on endothelial cell density of porcine corneas in organ culture medium containing dextran with regards to possible negative influences of high temperatures during the storage and transport of corneal grafts. Four groups of central discs (diameter 8 mm) from the corneas of both eyes in 40 pigs were first organ-cultured (MEM with 6% dextran 500) for 24 h at 32°C. Ten corneas were then exposed to 40°C in group 1, to 42°C in group 2, to 44°C in group 3, and to 50°C in group 4 for 12 h each. The paired corneal discs for all groups were not treated, stored at 32°C and served as controls. After further organ culture of all corneas for 48 h at 32°C to allow regenerative processes, corneal endothelium was stained with Alizarin Red S and examined by light microscopy. The endothelial cell densities were determined on three central images using a system for the automatic estimation of morphometric parameters of corneal endothelium. Exposure for 12 h to 40°C as well as to 42°C induced no endothelial cell loss. Statistical analysis showed no significant difference of the endothelial cell density between corneas exposed to 40°C and 42°C and the control corneas (40°C treatment: 4736 ± 426 cells/mm(2) and control: 4762 ± 344 cells/mm(2), p = 0.74; 42°C treatment: 4240 ± 363 cells/mm(2) and control: 4176 ± 448 cells/mm(2), p = 0.40). Exposure to 44°C and 50°C lead to total necrosis of the endothelial cell layer. Exposure of organ cultured porcine corneas in dextran containing medium up to 42°C for 12 h does not compromise the endothelial cell density in a clinically relevant manner. Temperatures above 42°C, as it might be the case during transports from the cornea bank to the ophthalmic surgeon, must be strictly avoided as they damage the endothelial cell layer.

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Section: Introductionmentioning

confidence: 99%

Impact of temporary hyperthermia on corneal endothelial cell survival during organ culture preservation

Schroeter¹,

Ruggeri

Thieme

et al. 2015

Graefes Arch Clin Exp Ophthalmol

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“…In one such study, the quality control in an eye bank setting was carried out to validate the entire process from cornea procurement up to transplantation 6. An uncontrolled change of temperature, which may occur for example during transportation, can exert a potentially harmful non-physiological influence on the corneal endothelial cells 7. In order to study the effects of significant changes of the storage temperature in organ cultured corneas, an investigation was performed where alizarine red-stained images were acquired and analysed by corneal experts, who manually outlined, that is, segmented, the cell contours to derive an estimation of the morphometric parameters 6…”

Section: Introductionmentioning

confidence: 99%

A system for the automatic estimation of morphometric parameters of corneal endothelium in alizarine red-stained images

Ruggeri

Scarpa

Luca

et al. 2010

British Journal of Ophthalmology

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BACKGROUND/AIMS A computer program for the automatic estimation of endothelium morphometric parameters (cell density, pleomorphism, polymegethism) in alizarine red-stained images is presented and evaluated. METHODS Images of corneal endothelium from 30 porcine eyes stained with alizarine red were acquired with an optical microscope and saved as grey-level digital images. Each image was first pre-processed for luminosity correction and contrast enhancement. An artificial neural network was used to classify all pixels as cell contour or cell body pixels. The segmented cell contours were then used to obtain estimates of morphometric parameters. The central area was assessed and the mean area per cornea was 0.54+/-0.07 mm(2). The whole system was implemented as a computer program using the Matlab language. Estimated parameters were compared with the corresponding values derived from manual contour detection on the same images used for the automatic estimation. RESULTS For the 30 images in our dataset, the mean differences for automatic versus manual parameters were -12+/-52 (range -103 to +145) cells/mm(2) for density, 0.5+/-2.6% (range -5.6 to +5.6%) for pleomorphism and -0.7+/-1.9% (range -4.1 to +2.8%) for polymegethism. CONCLUSION The evaluation of the automatic system on 30 images from porcine eyes confirmed its ability to estimate reliably morphometric parameters with respect to parameter values derived by manual analysis.

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“…However, the conditions of tissue during transportation have not been standardized, even though reevaluation of the corneas after transportation is not common [6,17,22]. An uncontrolled change of temperature can occur during transportation, and may exert an unphysiological influence on the corneal endothelial cells that is potentially harmful [11,13]. On the one hand, it seems to be unlikely that the level of temperature strongly influences cell number and vitality of the endothelium, since different incubation temperatures (range between 4°C and 37°C) for preservation have been reported in the literature [9,10,14,21].…”

Section: Introductionmentioning

confidence: 99%

Influence of temporary hypothermia on corneal endothelial cell density during organ culture preservation

Schroeter

Meltendorf

Ohrloff

et al. 2007

Graefes Arch Clin Exp Ophthalmol

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Exposure of organ-cultured porcine corneas in dextran containing medium to 4 degrees C for 12 hours and 21 degrees C for 48 hours does not compromise the endothelial cell density of donor corneas in a clinically relevant manner. A storage of corneal grafts at temperatures down to 4 degrees C for 12 hours, as might be the case during transport from the cornea bank to the ocular surgeon, does not seem to damage the endothelial cell layer.

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Methods of refrigerated transport of corneas for transplantation

Cited by 3 publications

References 1 publication

Impact of temporary hyperthermia on corneal endothelial cell survival during organ culture preservation

Impact of temporary hyperthermia on corneal endothelial cell survival during organ culture preservation

A system for the automatic estimation of morphometric parameters of corneal endothelium in alizarine red-stained images

Influence of temporary hypothermia on corneal endothelial cell density during organ culture preservation

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