Methods of Screening-Purification and Antimicrobial Potentialities of Bacteriocin in Health Care

Ahmad, Varish; Jamal, Qazi Mohammad Sajid; Siddiqui, Mughees Uddin; Shukla, Arun Kumar; Alzohairy, Mohammad A.; Karaawi, Mohammad A. Al; Kamal, Mohammad Amjad

doi:10.2174/1389200218666170116111309

Cited by 6 publications

(9 citation statements)

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“…Therefore, it is deduced that bacteriocins indirectly protect the host from infectious microbial pathogens and enhance the immune system. Funderburg et al (2007) found that the bacteriocin secreted by L. plantarum affected the immune response of dendritic cells, whereas de Pablo et al (1999) found that Nisaplin (a preparation containing 2.5% nisin, 77.5% NaCl, and skimmed milk powder) can increase CD4 + and CD8 + T lymphocytes and decrease B lymphocytes to regulate the adaptive immune response. Lee et al (2020) identified eight genetic loci of L. plantarum WCFS1 and found that three of the bacteriocin loci can regulate the immune response of DC cells via simulating the secretion levels of IL-10 (3.3 times) and IL-12 (2.4 times).…”

Section: Bacteriocins For Intestinal Immunitymentioning

confidence: 99%

“…coli is generally not pathogenic and is a resident bacterium in human and animal intestines. However, even normal "non-pathogenic" E. coli strains can cause infection under immunosuppressed circumstances or when the GI barrier is damaged (Al-Shabib et al, 2017), which can lead to urinary tract infection, sepsis, meningitis, and diarrhea. Luo et al (2020) studied the antibacterial mechanism of bacteriocin BM1157 against E. coli and Cronobacter sakazakii, and they found that the membrane integrity and permeability of the two indicator strains were not affected after BM1157 treatment.…”

Section: Bm1157mentioning

confidence: 99%

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Bacteriocins: Curial guardians of gastrointestinal tract

Gu,

Yan,

Lou

et al. 2024

Comp Rev Food Sci Food Safe

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The human gastrointestinal (GI) tract microbiome secretes various metabolites that play pivotal roles in maintaining host physiological balance and influencing disease progression. Among these metabolites, bacteriocins—small, heat‐stable peptides synthesized by ribosomes—are notably prevalent in the GI region. Their multifaceted benefits have garnered significant interest in the scientific community. This review comprehensively explores the methods for mining bacteriocins (traditional separation and purification, bioinformatics, and artificial intelligence), their effects on the stomach and intestines, and their complex bioactive mechanisms. These mechanisms include flora regulation, biological barrier restoration, and intervention in epithelial cell pathways. By detailing each well‐documented bacteriocin, we reveal the diverse ways in which bacteriocins interact with the GI environment. Moreover, the future research direction is prospected. By further studying the function and interaction of intestinal bacteriocins, we can discover new pharmacological targets and develop drugs targeting intestinal bacteriocins to regulate and improve human health. It provides innovative ideas and infinite possibilities for further exploration, development, and utilization of bacteriocins. The inevitable fact is that the continuously exploration of bacteriocins is sure to bring the promising future for demic GI health understanding and interference strategy.

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Section: Bacteriocins For Intestinal Immunitymentioning

confidence: 99%

Section: Bm1157mentioning

confidence: 99%

Bacteriocins: Curial guardians of gastrointestinal tract

Gu,

Yan,

Lou

et al. 2024

Comp Rev Food Sci Food Safe

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“…The bactericidal mechanism of bacteriocins is closely related to the class of bacteriocins and indicator bacteria, which differ from antibiotics. These mechanisms can be divided into cell envelope and inhibition of gene expression (Ahmad et al., 2017; Cotter et al., 2013; Johnson et al., 2018; Kumariya et al., 2019) (Figure 3).…”

Section: Antibacterial Mechanismmentioning

confidence: 99%

Mining, heterologous expression, purification, antibactericidal mechanism, and application of bacteriocins: A review

Cui

Luo

Wang

et al. 2020

Comp Rev Food Sci Food Safe

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Bacteriocins are generally considered as low‐molecular‐weight ribosomal peptides or proteins synthesized by G+ and G− bacteria that inhibit or kill other related or unrelated microorganisms. However, low yield is an important factor restricting the application of bacteriocins. This paper reviews mining methods, heterologous expression in different systems, the purification technologies applied to bacteriocins, and identification methods, as well as the antibacterial mechanism and applications in three different food systems. Bioinformatics improves the efficiency of bacteriocins mining. Bacteriocins can be heterologously expressed in different expression systems (e.g., Escherichia coli, Lactobacillus, and yeast). Ammonium sulfate precipitation, dialysis membrane, pH‐mediated cell adsorption/desorption, solvent extraction, macroporous resin column, and chromatography are always used as purification methods for bacteriocins. The bacteriocins are identified through electrophoresis and mass spectrum. Cell envelope (e.g., cell permeabilization and pore formation) and inhibition of gene expression are common antibacterial mechanisms of bacteriocins. Bacteriocins can be added to protect meat products (e.g., beef and sausages), dairy products (e.g., cheese, milk, and yogurt), and vegetables and fruits (e.g., salad, apple juice, and soybean sprouts). The future research directions are also prospected.

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“…Bacteriocins have been tested as safe and effective alternative molecules over the currently used chemotherapeutic agents. Thus, being an important clinical significance, its screening and recovery methods along with its application are poorly described ( 11 ). According to recent information on functional changes in commensal bacterial species in the gut, in this study we tried to investigate the changes in Lactobacillus species population and bacteriocins production as one of the more important probiotic strains, in new cases of IBD and symptomatically managed patients in comparison to healthy people.…”

Section: Introductionmentioning

confidence: 99%

Comparison of the prevalence of bacteriocin encoding genes in Lactobacillus spp. isolated from fecal samples of healthy volunteers, IBD-patient and IBD-recovered

Miri¹,

Sotoodehnejadnematalahi²,

Amiri³

et al. 2022

IJM

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Background and Objectives: Bacteriocins are antimicrobial peptides produced by many genera of bacteria especially Lac- tobacillus spp. against many pathogens, adapt bacterial composition in the gut and inhibit dysbiosis that can lead to inflam- mation disorders like inflammatory bowel disease (IBD). The aim of this study was to compare the prevalence of bacteriocin genes in health, IBD disease and recovery conditions. Materials and Methods: In this survey 115 Lactobacillus spp. from 58 fecal samples of three different groups were evaluat- ed. Comparison of the presence of bacteriocin genes in different groups were assayed by purified samples and PCR method, followed by statistical analysis to identify the effect of inflammation in the proportion of Lactobacillus spp. and presence of their bacteriocin genomes. Results: Of 115 Lactobacillus spp. 60% of samples had positive bacteriocin-encoding genes which included: gassericin-A 29.56%, acidocin 15.65%, plantaricin-NC8 18.26%, plantaricin-S 13.04%, lactacin-F 9.5%, sakacin-P 6.08% and gasseri- cin-T 6.08%. Results indicated that the percentage of positive bacteriocin genes were much more in healthy volunteer and IBD-recovered in comparison to IBD-patients which showed the effect of inflammation in the presence of bacteriocin genes. Conclusion: The results obtained in this study demonstrated that the presence of bacteriocin genes can be related to health and disease states and inflammatory disease affected the prevalence of bacteriocin-encoding genes. This approach can help to identify bacterial functions that can be targeted in future concepts of IBD therapy.

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Methods of Screening-Purification and Antimicrobial Potentialities of Bacteriocin in Health Care

Cited by 6 publications

References 0 publications

Bacteriocins: Curial guardians of gastrointestinal tract

Bacteriocins: Curial guardians of gastrointestinal tract

Mining, heterologous expression, purification, antibactericidal mechanism, and application of bacteriocins: A review

Comparison of the prevalence of bacteriocin encoding genes in Lactobacillus spp. isolated from fecal samples of healthy volunteers, IBD-patient and IBD-recovered

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