Methods to isolate extracellular vesicles for diagnosis

Kang, Hyejin; Kim, Jiyoon; Park, Jaesung

doi:10.1186/s40486-017-0049-7

Cited by 68 publications

(73 citation statements)

References 98 publications

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“…However, the efficiency of the method is lower when viscous biological fluids such as plasma and serum are used for analysis [ 41 ]. The quantity of collected EVs is affected by centrifuging speed and time, so these parameters must be optimized for each rotor type [ 42 ].…”

Section: Introductionmentioning

confidence: 99%

Recent advancements in the use of exosomes as drug delivery systems

et al. 2018

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Extracellular vesicles (EVs) are the substances that are released by most types of cells and have an important role in cell to cell communication. Among the most highly researched EVs are exosome. Recent studies show that exosomes derived from cells have different roles and targets. Many studies show that exosome can efficiently deliver many different kinds of cargo to the target cell. Therefore, they are often used to deliver therapeutic cargo for treatment. The exosomes that have been used include both natural ones and those that have been modified with other substances to increase the delivery ability. This article provides a review of both exosomes derived from various cells and modified exosome and their ability in delivering the many kinds of cargo to the target cell.

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Section: Introductionmentioning

confidence: 99%

Recent advancements in the use of exosomes as drug delivery systems

et al. 2018

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“…Circulating plasma EVs were isolated using ultracentrifugation. 16,17 Briefly, debris and platelets were removed from the plasma by centrifugation at 2000g for 10 minutes. Platelet-free plasma was then centrifuged first at 3000g for 10 minutes and then transferred to a new tube for centrifugation at 100 000g for 60 minutes at 4°C.…”

Section: Patients and Samplesmentioning

confidence: 99%

Quantitative proteomics of plasma vesicles identify novel biomarkers for hemoglobin E/β-thalassemic patients

et al. 2018

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Key Points• Chaperones, antioxidants, ironsequestering proteins, and cathepsin S exhibited increased abundance in thalassemic EVs.• Haptoglobin and hemopexin are reduced in thalassemic patients' EVs, reflecting hemolysis. These could be used as clinical biomarkers.Hemoglobin E (HbE)/b-thalassemia has a wide spectrum of clinical manifestations that cannot be explained purely by its genetic background. Circulating extracellular vesicles (EVs) are one factor that likely contributes to disease severity. This study has explored the differences in protein composition and quantity between EVs from HbE/b-thalassemic patients and healthy individuals. We used tandem mass tag labeling mass spectrometry to analyze the EV proteins isolated from the plasma of 15 patients compared with the controls.To reduce biological variation between individuals, the EV proteins isolated from randomly assigned groups of 5 HbE/b-thalassemic patients were pooled and compared with 5 pooled age-and sex-matched controls in 3 separate experiments. Alpha hemoglobin-stabilizing protein had the highest fold increase. Catalase, superoxide dismutase, T-complex proteins, heat shock proteins, transferrin receptor, ferritin, and cathepsin S were also upregulated in thalassemic circulating EVs. Importantly, haptoglobin and hemopexin were consistently reduced in patients' EVs across all data sets, in keeping with the existing hemolysis that occurs in thalassemia. The proteomic data analysis of EV samples isolated from 6 individual HbE/b-thalassemic patients and western blotting results corroborated these findings. In conclusion, we have successfully identified consistent alterations of protein quantity between EVs from HbE/b-thalassemic and healthy individuals. This work highlights haptoglobin, hemopexin, and cathepsin S as potential clinically relevant biomarkers for levels of hemolysis and inflammation. Monitoring of these plasma proteins could help in the clinical management of thalassemia.

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“…Each method exploits a specific trait of EXs, such as their size, shape, density and surface antigens to aid isolation. However, the most challenging task of all methods employed is to specifically purify EXs from the wide spectrum of EVs, cellular debris and interfering molecular components [76,77]. For this reason, the quality of isolated EXs should be examined each time before any further application is persued.…”

Section: Isolation Methodsmentioning

confidence: 99%

Exosomes and Exosome-Inspired Vesicles for Targeted Drug Delivery

Antimisiaris

Mourtas

Marazioti

2018

Preprint

155

125

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The similarities between exosomes and liposomes, together with the high organotropism of several types of exosomes, have recently prompted the development of engineered-exosomes or exosome-mimetics, which may be artificial (liposomal) or cell-derived vesicles, as advanced platforms for targeted drug delivery. Here we provide the current state-of-the-art of using exosome or exosome-inspired systems for drug delivery. We review the various approaches investigated and the shortcomings of each approach. Finally the challenges identified up-to-date in this field are summarized.

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Methods to isolate extracellular vesicles for diagnosis

Cited by 68 publications

References 98 publications

Recent advancements in the use of exosomes as drug delivery systems

Recent advancements in the use of exosomes as drug delivery systems

Quantitative proteomics of plasma vesicles identify novel biomarkers for hemoglobin E/β-thalassemic patients

Exosomes and Exosome-Inspired Vesicles for Targeted Drug Delivery

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