Methods Used for the Detection of Antimutagens: An Overview

Nersesyan, Armen; Mišík, Miroslav; Knasmüller, Siegfried

doi:10.1002/9783527626588.ch14

Cited by 2 publications

(1 citation statement)

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“…Short-term bacterial mutation assays, such as the Ames assay, are an effective screening tool for the identification of various mutagenic or antimutagenic compounds in complex materials [17]. The assay has advantages as an inexpensive and flexible screening method that provides preliminary information related to antimutagenesis.…”

Section: Introductionmentioning

confidence: 99%

Isolation and characterization of antimutagenic components of Glycyrrhiza aspera against N-methyl-N-nitrosourea

Inami

Mine

Tatsuzaki³

et al. 2017

Genes and Environ

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BackgroundA powdered ethanolic extract of Glycyrrhiza aspera root exhibits antimutagenic activity against N-methyl-N-nitrosourea (MNU) based on the Ames assay with Salmonella typhimurium TA1535. The aim of this study was to identify the antimutagenic components of the powdered ethanolic extract of G. aspera root.ResultsThe powdered ethanolic extract of G. aspera root was sequentially suspended in n-hexane, carbon tetrachloride, dichloromethane, ethyl acetate, and ethanol, and each solvent soluble fraction and the residue were assayed for antimutagenic activity against MNU in S. typhimurium TA1535. The dichloromethane soluble fraction exhibited the highest antimutagenicity and was fractionated several times by silica gel chromatography. The fraction with the highest antimutagenic activity was further purified using HPLC, and the fractions were assayed for antimutagenicity against MNU in S. typhimurium TA1535. Finally, five components with antimutagenic activity against MNU were identified as glyurallin A, glyasperin B, licoricidin, 1-methoxyphaseollin, and licoisoflavone B.ConclusionsThe five components were demonstrated to possess an antigenotoxic effect against carcinogenic MNU for the first time. It is important to prevent DNA damage by N-nitrosamines for cancer chemoprevention.Electronic supplementary materialThe online version of this article (doi:10.1186/s41021-016-0068-2) contains supplementary material, which is available to authorized users.

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Section: Introductionmentioning

confidence: 99%