Methotrexate induces high level of apoptosis in canine lymphoma/leukemia cell lines

Pawlak, Aleksandra; Kutkowska, Justyna; Obmińska-Mrukowicz, Bożena; Rapak, Andrzej

doi:10.1016/j.rvsc.2017.09.026

Cited by 19 publications

(16 citation statements)

References 24 publications

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“…MTT assay. Cell proliferation was measured by MTT assay (20). Briefly, cells at a density of 1×10 4 /well (CL-1, CLBL-1) and 1.5×10 3 /well (D-17) were seeded in a 96-well-plate (TPP, Trasadingen, Switzerland) and pre-incubated at 37˚C under 5% CO 2 for 24 h. Then, various concentrations of BA or BT (1, 5, 10, 15, 20, 25, 30 and 40 μM) were added and the samples were further incubated for 24 h, 48 h and 72 h. After incubation, 20 μl of 5 mg/ml MTT were added to each well and the samples were left for 4 h. After this time 80 μl of a lysis buffer (225 ml DMF, 67.5 g SDS, 275 ml distilled water) were added.…”

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confidence: 99%

Antitumor Activity of Betulinic Acid and Betulin in Canine Cancer Cell Lines

Zhao

Pawlak

et al. 2018

In Vivo

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confidence: 99%

Antitumor Activity of Betulinic Acid and Betulin in Canine Cancer Cell Lines

Zhao

Pawlak

et al. 2018

In Vivo

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“…Methotrexate effects folic acid pathway [2], as it closely resembles folic acid, acting as antimetabolite and used in the treatment of various diseases like arthritis and cancer [3]. Although methotrexate has many applications but it has certain disadvantages, e.g.…”

Section: Aminopterinmentioning

confidence: 99%

A comprehensive review of synthesized derivatives of methotrexate in relation to their anticancer potential.

Nemat¹,

Iqbal²,

Mehmood³

2020

medical-oncology

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Methotrexate (MTX) is a chemotherapeutic agent with certain side effects. Efforts were made to synthesize analogues of MTX to enhance the activity and reduce side effects. Various derivatives were synthesized by structural modification of parent drug and mainly assayed for anticancer activity and binding affinity with dihydrofolatereductase (DHFR) and folylpolyglutamate synthetase (FPGS). In vitro study was carried out on cell lines CCRF. CEM, ATCC8, L. Casei, P388, L1210, L1210/R81, and H35. These synthesized derivatives have been assayed for human squamous cell carcinoma namely SSC25, SSC68, SSC78, SSC25/R1, SSC68/R1, and SSC78/R1. Some modification proved to enhance anticancer activity while others are detrimental. Some of derivatives were also tested for other biological activity like anti-malarial, anti-bacterial and anti-diabetic and show better activity. This article deals with a comprehensive overview of the synthesized derivatives by structural modifications and the impact of these modifications on its activity.

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“…Apoptosis is a severe toxic phenomenon marked by cell shrinkage, chromatin condensation, nuclear DNA fragmentation, a decrease in the mitochondrial membrane potential (MMP) and caspase activation . MTX exerts its antitumour effects via the induction of apoptosis in many cancers . Unfortunately, it can also induce apoptosis in healthy tissues, including the testes, and the underlying molecular mechanism is poorly characterized.…”

Section: Introductionmentioning

confidence: 99%

“…9 MTX exerts its antitumour effects via the induction of apoptosis in many cancers. [10][11][12] Unfortunately, it can also induce apoptosis in healthy tissues, including the testes, 13,14 and the underlying molecular mechanism is poorly characterized. Autophagy is an intracellular system that degrades damaged cytoplasmic components and proteins to eliminate old and unhealthy cells.…”

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confidence: 99%

Reactive oxygen species, not Ca²⁺, mediates methotrexate‐induced autophagy and apoptosis in spermatocyte cell line

Xiong

Song

Yang

et al. 2019

Basic Clin Pharma Tox

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Methotrexate (MTX), a folic acid antagonist, is used to treat various malignancies and systemic autoimmune diseases because it has unique antiproliferative, antimetabolic and anti-inflammatory properties. 1 The underlying mechanism responsible for these effects may involve preventing nucleotide synthesis through MTX reaction with dihydrofolate reductase (DHFR), with subsequent impairment of DNA and RNA synthesis, as DHFR is necessary for nucleotide anabolism. 2 Although chemotherapy drugs such as MTX are mainly used to inhibit the proliferation of cancer cells, it also affects healthy tissues and cells, resulting in functional disorders of both somatic and reproductive cells. 3,4 Studies in animal models have demonstrated that MTX is cytotoxic to

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Methotrexate induces high level of apoptosis in canine lymphoma/leukemia cell lines

Cited by 19 publications

References 24 publications

Antitumor Activity of Betulinic Acid and Betulin in Canine Cancer Cell Lines

Antitumor Activity of Betulinic Acid and Betulin in Canine Cancer Cell Lines

A comprehensive review of synthesized derivatives of methotrexate in relation to their anticancer potential.

Reactive oxygen species, not Ca²⁺, mediates methotrexate‐induced autophagy and apoptosis in spermatocyte cell line

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Methotrexate induces high level of apoptosis in canine lymphoma/leukemia cell lines

Cited by 19 publications

References 24 publications

Antitumor Activity of Betulinic Acid and Betulin in Canine Cancer Cell Lines

Antitumor Activity of Betulinic Acid and Betulin in Canine Cancer Cell Lines

A comprehensive review of synthesized derivatives of methotrexate in relation to their anticancer potential.

Reactive oxygen species, not Ca2+, mediates methotrexate‐induced autophagy and apoptosis in spermatocyte cell line

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Reactive oxygen species, not Ca²⁺, mediates methotrexate‐induced autophagy and apoptosis in spermatocyte cell line