Methyl jasmonate dramatically enhances the accumulation of phenolic acids in <i>Salvia miltiorrhiza</i> hairy root cultures

Xiao, Ying; Gao, Shouhong; Di, Peng; Chen, Junfeng; Chen, Wansheng; Zhang, Lei

doi:10.1111/j.1399-3054.2009.01257.x

Cited by 148 publications

(120 citation statements)

References 24 publications

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“…Recently, another Arabidopsis TyrAT gene (At5g36160; designated here as AtTyrAT-4) was reported as an aminotransferase capable of interconverting L-Tyr and 4-HPP as well as and L-Phe and phenylpyruvate (Prabhu and Hudson, 2010). The transcript abundance of TyrAT in crude protein extracts of S. miltiorrhiza hairy root cultures increased along with Phe ammonia lyase, cinnamic acid 4-hydroxylase, 4-hydroxyphenylpyruvate reductase (HPPR), and 4-hydroxyphenylpyruvate dioxygenase (HPPD) transcript levels in response to MeJA treatment (Xiao et al, 2009b). TyrAT with a high substrate specificity for Tyr and broad specificity toward amino group acceptors was purified from rosmarinic acid-producing cell cultures of A. officinalis and C. blumei (De-Eknamkul and Ellis, 1987b), and an increase in TyrAT activity in response to MeJA treatment was accompanied by higher a-tocopherol levels in A. caudatus cell cultures (Antognoni et al, 2009).…”

Section: Involvement Of Tyrat In Bia Metabolism In Opium Poppymentioning

confidence: 99%

“…TyrAT activity was reported in rosmarinic acid-producing cell cultures of Anchusa officinalis and Coleus blumei and in MeJAtreated hairy root cultures of Salvia miltiorrhiza (DeEknamkul and Ellis, 1987a;Xiao et al, 2009b). In plants, tocopherols and rosmarinic acid function as free radical scavengers and confer protection against a variety of biotic and abiotic environmental stress factors (Liu et al, 1992;Sattler et al, 2004;Xiao et al, 2009b). These natural products are also associated with potential benefits to human health.…”

mentioning

confidence: 99%

“…TyrAT is regulated by coronatine, wounding, and methyl jasmonate (MeJA) and has been implicated as the initial enzyme in tocopherol biosynthesis in Arabidopsis (Arabidopsis thaliana) plants (Lopukhina et al, 2001;Holländer-Czytko et al, 2005) and Amaranthus caudatus and Chenopodium quinoa cell cultures (Antognoni et al, 2009). TyrAT activity was reported in rosmarinic acid-producing cell cultures of Anchusa officinalis and Coleus blumei and in MeJAtreated hairy root cultures of Salvia miltiorrhiza (DeEknamkul and Ellis, 1987a;Xiao et al, 2009b). In plants, tocopherols and rosmarinic acid function as free radical scavengers and confer protection against a variety of biotic and abiotic environmental stress factors (Liu et al, 1992;Sattler et al, 2004;Xiao et al, 2009b).…”

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confidence: 99%

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Tyrosine Aminotransferase Contributes to Benzylisoquinoline Alkaloid Biosynthesis in Opium Poppy

Lee

Facchini

2011

Plant Physiology

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Tyrosine aminotransferase (TyrAT) catalyzes the transamination of L-Tyr and a-ketoglutarate, yielding 4-hydroxyphenylpyruvic acid and L-glutamate. The decarboxylation product of 4-hydroxyphenylpyruvic acid, 4-hydroxyphenylacetaldehyde, is a precursor to a large and diverse group of natural products known collectively as benzylisoquinoline alkaloids (BIAs). We have isolated and characterized a TyrAT cDNA from opium poppy (Papaver somniferum), which remains the only commercial source for several pharmaceutical BIAs, including codeine, morphine, and noscapine. TyrAT belongs to group I pyridoxal 5#-phosphate (PLP)-dependent enzymes wherein Schiff base formation occurs between PLP and a specific Lys residue. The amino acid sequence of TyrAT showed considerable homology to other putative plant TyrATs, although few of these have been functionally characterized. Purified, recombinant TyrAT displayed a molecular mass of approximately 46 kD and a substrate preference for L-Tyr and a-ketoglutarate, with apparent K m values of 1.82 and 0.35 mM, respectively. No specific requirement for PLP was detected in vitro. Liquid chromatography-tandem mass spectrometry confirmed the conversion of L-Tyr to 4-hydroxyphenylpyruvate. TyrAT gene transcripts were most abundant in roots and stems of mature opium poppy plants. Virus-induced gene silencing was used to evaluate the contribution of TyrAT to BIA metabolism in opium poppy. TyrAT transcript levels were reduced by at least 80% in silenced plants compared with controls and showed a moderate reduction in total alkaloid content. The modest correlation between transcript levels and BIA accumulation in opium poppy supports a role for TyrAT in the generation of alkaloid precursors, but it also suggests the occurrence of other sources for 4-hydroxyphenylacetaldehyde.

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Section: Involvement Of Tyrat In Bia Metabolism In Opium Poppymentioning

confidence: 99%

mentioning

confidence: 99%

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Tyrosine Aminotransferase Contributes to Benzylisoquinoline Alkaloid Biosynthesis in Opium Poppy

Lee

Facchini

2011

Plant Physiology

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“…As a new type of plant hormone, the influence of JA on secondary metabolism has been widely studied (Zhou and Memelink, 2016). Methyl-JA was used to affect the accumulation of phenolic acids in Salvia miltiorrhiza (Xiao et al, 2009) and tartary buckwheat (Gumerova et al, 2015), while anthocyanins and PAs were affected when various jasmonates were applied to common buckwheat (Horbowicz et al, 2009). The JAZ (jasmonate ZIM-domain) proteins are important repressors of the JA pathway (Chini et al, 2007;Shan et al, 2007;Pauwels and Goossens, 2011).…”

Section: Discussionmentioning

confidence: 99%

The jasmonate-ZIM domain protein FtJAZ2 interacts with the R2R3-MYBtranscription factor FtMYB3 to affect anthocyanin biosynthesis in tartary buckwheat

Luo¹,

Li²,

Yao³

et al. 2017

Turk J Biol

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Tartary buckwheat is a type of coarse cereal that contains a rich supply of flavonoids. When suffering from certain stresses, tartary buckwheat can respond by promoting the biosynthesis of anthocyanins. It is well established that the jasmonate (JA) signaling pathway plays a critical role in plant resistance to disease by stimulating the production of anthocyanins; furthermore, the genes encoding the jasmonate-ZIM domain (JAZ) proteins have been shown to be key players in this process. However, the role of JAZ proteins in tartary buckwheat is poorly understood. In this study, we aimed to isolate FtJAZs from tartary buckwheat and identify FtJAZs that are involved in anthocyanin biosynthesis under cold-stress conditions based on an interaction with the known FtMYBs. We identified three novel FtJAZ genes in tartary buckwheat. According to a phylogenetic analysis, FtJAZ1 was organized into cluster I, while FtJAZ2 and FtJAZ3 were organized into cluster II. Interestingly, a yeast two-hybrid assay showed that FtMYB3 interacted only with FtJAZ2. Furthermore, our qRT-PCR results suggested that FtJAZ2, dihydroflavonol-4-reductase, and anthocyanin synthase had similar expression patterns, which is consistent with the accumulation of anthocyanins. Conversely, the expression of FtMYB3 showed an opposite trend, being negatively correlated with the anthocyanin level. In conclusion, by interacting with FtMYB3, FtJAZ2 plays a role in anthocyanin biosynthesis under cold stress.

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“…The products of real time quantitative PCR were run on 1.5% agarose gelelectrophoresis and showed an equal-sized band as predicted. Quantification of the gene expression was done with comparative CT method (Xiao et al, 2009 …”

Section: Rna Isolation and Real-time Quantitative Pcr Analysismentioning

confidence: 99%

La Dramaticaly Enhances the Accumulation of Tanshinones in Salvia Miltiorrhiza Hairy Root Cultures

Zhou¹,

Fang²,

Wang³

et al. 2012

ESR

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The accumulation of tanshinones (tanshinone I, tanshinone II and cryptotanshinone) and the expression of key enzymes (HMGR， HMGS， KSL， DXR， CMK) of tanshinones biosynthetic pathways in Salvia miltiorrhiza hairy root cultures induced by La elicitor were investigated in this paper. La at 0.01mmol·L -1 and 0.1mmol·L -1 obviously stimulated the expression of HMGR on day 1 after treatment. The activity of HMGS and KSL was enhanced by La treatment at the earlier stage of the elicitation treatment and declined gradually at later stage. The activity of CMK was significantly improved with the treatment of La at dilution of 0.01mmol·L -1 on day 1 after treatment. La at 0.01mmol·L -1 and 0.1mmol·L -1 improved the activity of DXR by 161.8% and 378.4% respectively on day 3 after treatment. The content of tanshinone I was markedly enhanced by La at 0.01mmol·L -1 and 0.1mmol·L -1 on day 1 after treatment. La at 0.01mmol·L -1 enhanced the content of tanshinone IIA, which was estimated to be 40.9% higher than the control. The content of cryptotanshinone was significantly improved by 92.4% and 71.5% respectively on day 1 and 3 after treatment with La at 0.01mmol·L -1 , while it was reduced by La at 0.01mmol·L -1 , 0.1mmol·L -1 and 1mmol·L -1 7 days after treatment. In summary the accumulation of tanshinones and expression of key enzymes of tanshinones biosynthetic pathways were improved by La elicitor with lower dose while inhibited with higher dose. And the effect of La on the accumulation of tanshinones lagged behind its effect on the expression of key enzyme gene.

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Methyl jasmonate dramatically enhances the accumulation of phenolic acids in Salvia miltiorrhiza hairy root cultures

Cited by 148 publications

References 24 publications

Tyrosine Aminotransferase Contributes to Benzylisoquinoline Alkaloid Biosynthesis in Opium Poppy

Tyrosine Aminotransferase Contributes to Benzylisoquinoline Alkaloid Biosynthesis in Opium Poppy

The jasmonate-ZIM domain protein FtJAZ2 interacts with the R2R3-MYBtranscription factor FtMYB3 to affect anthocyanin biosynthesis in tartary buckwheat

La Dramaticaly Enhances the Accumulation of Tanshinones in Salvia Miltiorrhiza Hairy Root Cultures

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