Methyl-paraoxon comparative inhibition kinetics for acetylcholinesterases from brain of neotropical fishes

Filho, Moacelio Verânio Silva; Oliveira, Marcos L.S.; Salles, João Bosco de; Bastos, V. L. F. Cunha; Cassano, Valéria; Bastos, J. Cunha

doi:10.1016/j.toxlet.2004.04.026

Cited by 46 publications

(17 citation statements)

References 14 publications

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“…The same was reported in several studies on freshwater and marine fish (42)(43)(44). For instance, Hernández-Moreno et al (43) observed that brain and muscle cholinesterase activities in seabass (Dicentrarchus labrax) exposed to 250 mg L -1 of carbofuran were significantly inhibited, showing a 47 % of inhibition in brain and 41 % in muscle, compared to the control.…”

Section: Figure 1 Levels Of Catalase (Cat) Glutathione-s-transferasesupporting

confidence: 74%

Different Enzymatic Activities in Carp (Cyprinus Carpio L.) as Potential Biomarkers of Exposure to the Pesticide Methomyl

Hernández‐Moreno

Resino

Flores

et al. 2014

Archives of Industrial Hygiene and Toxicology

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This study investigated the influence of the pesticide methomyl on different enzymatic activities in carp. The fish were exposed to a sub-lethal concentration (0.34 mg L -1 ) of methomyl for 15 days. On days 4 and 15, catalase (CAT) and glutathione-S-transferase (GST) activities were measured in the liver and gills. Acetylcholinesterase (AChE) activity in brain and muscle was also determined. Liver catalase activity slightly increased in exposed fish when compared to controls, but it was statistically significant only at the beginning of the experiment. No changes in CAT activity in the gills of exposed and control animals were observed (mean values were in the range 10.7-11.7 nmol min -1 per mg of protein). Liver GST activity was slightly inhibited in the exposed animals at the beginning of the study; however, it was significantly inhibited in the gills. Brain AChE activity was diminished throughout the experiment and significantly decreased after 96 h of exposure compared to controls (0.041 vs. 0.075 nmol min -1 per mg of protein; p<0.001). Our findings suggest that CAT, GST, and AChE are reliable biomarkers of effect after exposure to methomyl.

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Section: Figure 1 Levels Of Catalase (Cat) Glutathione-s-transferasesupporting

confidence: 74%

Different Enzymatic Activities in Carp (Cyprinus Carpio L.) as Potential Biomarkers of Exposure to the Pesticide Methomyl

Hernández‐Moreno

Resino

Flores

et al. 2014

Archives of Industrial Hygiene and Toxicology

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“…Brain tissue showed the highest pesticide bioconcentration, reaching 80 ppm after 30 minutes of exposure to methyl parathion ( Figure 2 ). The three fish species selected for this study present different brain cholinesterase sensitivities to intoxication by methyl paraoxon [ 15 ]. In these fishes, the sensitivity of brain ChE to intoxication by methyl paraoxon is inversely related to the activity of the brain enzyme for ASCho [ 15 ], in contrast to what was described for trout and rat by Kemp and Wallace [ 16 ].…”

Section: Discussionmentioning

confidence: 99%

Bioconcentration and Acute Intoxication of Brazilian Freshwater Fishes by the Methyl Parathion Organophosphate Pesticide

Salles

Lopes

Salles

et al. 2015

BioMed Research International

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Three species of freshwater Brazilian fishes (pacu, Piaractus mesopotamicus; piavussu, Leporinus macrocephalus, and curimbatá, Prochilodus lineatus) were exposed to an acute dose of 5 ppm methyl parathion organophosphate pesticide. Three to five individuals per species were exposed, one at a time, to 40 liters tap water spiked with Folidol 600. Pesticide concentrations and cholinesterase (ChE) activities were evaluated in serum, liver, brain, heart, and muscle. The bioconcentration of methyl parathion was similar for all studied fishes. Brain tissue showed the highest pesticide concentration, reaching 80 ppm after exposure for 30 min to methyl parathion. Three to 5 hours of 5 ppm methyl parathion exposure provoked the death of all P. lineatus at 92% brain AChE inhibition, whereas fish from the other two species survived for up to 78 hours with less than 80% brain AChE inhibition. Our results indicate that acute toxic effects of methyl parathion to fish are correlated with brain AChE sensitivity to methyl paraoxon.

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“…The method described by Silva Filho et al (2004) was used to determine the LOD of the ANAE inhibition method. Briefly, solutions of lettuce extracts (20 μl) with various OP concentrations that could inhibit an enzyme activity of 10%-90% were added to 500 μl centrifuge tubes.…”

Section: Enzyme Inhibition Assaymentioning

confidence: 99%

Determination of organophosphorus pesticide residues in vegetables by an enzyme inhibition method using α-naphthyl acetate esterase extracted from wheat flour

Wang

Xia

Zhang

et al. 2012

J. Zhejiang Univ. Sci. B

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Methyl-paraoxon comparative inhibition kinetics for acetylcholinesterases from brain of neotropical fishes

Cited by 46 publications

References 14 publications

Different Enzymatic Activities in Carp (Cyprinus Carpio L.) as Potential Biomarkers of Exposure to the Pesticide Methomyl

Different Enzymatic Activities in Carp (Cyprinus Carpio L.) as Potential Biomarkers of Exposure to the Pesticide Methomyl

Bioconcentration and Acute Intoxication of Brazilian Freshwater Fishes by the Methyl Parathion Organophosphate Pesticide

Determination of organophosphorus pesticide residues in vegetables by an enzyme inhibition method using α-naphthyl acetate esterase extracted from wheat flour

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