Methyl Parathion Degrading Enzyme‐based Nano‐hybrid Biosensor for Enhanced Methyl Parathion Recognition

Abstract

Enzyme‐based electrochemical biosensors with sufficient sensing specificity are useful analytical tools for detection of biologically important substances in complicated systems. Here, we present the design of a nano‐hybrid biosensor for the specific and sensitive detection of methyl parathion (MP). The nano‐hybrid sensing film was prepared via the formation of Au nanoparticals (AuNPs) on silica nanoparticles (SiNPs), mixing with multiwall carbon nanotube (MWNTs) and subsequent immobilization of methyl parathion degrading enzyme (MPD). The fabrication procedure was characterized by scanning electron images, linear scan voltammetry and electrochemical impedance spectroscopy. The combined MPD exhibited high affinity to it substrate and thus a selective, sensitive, fast and cheap method for determination of MP, quantitatively was proposed. A significant synergistic effect of nano‐hybrid on the biosensor performance was observed in biosensing MP. The square wave voltammetric responses displayed well defined peaks, linearly proportional to the concentration of MP in the range from 0.001 to 5.0 μg/mL with a detection limit of 0.3 ng/mL. The proposed biosensor also showed good precision and reproducibility, acceptable stability and accuracy in garlic samples analysis. It provided a platform for the simple and fast construction of biosensors with good performance for the determination of enzyme‐specific electroactive species.

Progress of optical biosensors for analyzing pathogens and organic pollutants in water since 2015

“…Silica based NPs are chemical inert, bio-compatible and optical transparent, and can maintain cell viability (Ye et al, 2016). Immobilization of microbial cells on SiNPs could be a way to produce a stable functional biohybrid material.…”

Applicability of Spray Drying Technique to Prepare Nano-Micro Carriers: A Review

“…Among various nanoparticles, silica nanoparticles have shown their superiority due to their characteristic features like biocompatibility, wide availability, ease of functionalization, high chemical, and mechanical stability, and high surface area [19][20][21]. Ponamoreva et al [22] have synthesized a stable functional biohybrid material by integrating yeast cells with silica for biosensor application.…”

Electrochemical biointerfaces based on carbon nanotubes-mesoporous silica hybrid material: Bioelectrocatalysis of hemoglobin and biosensing applications

“…To the best of our knowledge, this is the first report on the use of this hybrid material to develop thirdgeneration biosensors. So far, only a nanohybrid biosensor based on MWCNTs, AuNPs and silica nanoparticles, developed for the detection of methyl parathion (Ye et al, 2016), and a nanocomposite material of single-walled carbon nanotubes (SWCNTs) and gold-mesoporous silica Janus nanoparticles developed for the bienzymatic glucose biosensing (through the use of glucose oxidase (GOx), horseradish peroxidase (HRP) and hydroquinone as redox mediator) have been developed (Boujakhrout et al, 2015). The use of MWCNTs coated with mesoporous silica as signal tag for the electrochemical aptasensing of thrombin (Zhang et al, 2013) and mucin 1 (Chen et al, 2014) has been aso reported.…”