Methylation Profile of miR-9-1 and miR-9-1/-9-3 as Potential Biomarkers of Diabetic Retinopathy

Assis, Caroline Severo de; Silva, Alexandre; Nunes, Mayara Karla dos Santos; Filho, João Aragão Ximenes; Nascimento, Rayner Anderson Ferreira do; Gomes, Cecília Neta Alves Pegado; Evangelista, Isabella; Oliveira, Naila Francis Paulo de; Persuhn, Darlene Camati

doi:10.2174/1573399817666210101104326

Cited by 5 publications

(2 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…One study showed that miR-9 inhibits high glucose-induced proliferation, differentiation and collagen accumulation of cardiac fibroblasts by down-regulation of TGFBR2 [14]. Previous studies suggest that methylation of miR-9-1 and miR-9-3 favors diabetic retinopathy (DR) in patients with diabetes [15]. However, role of miR-9 in DCM has not reported.…”

Section: Introductionmentioning

confidence: 99%

Endothelial derived miRNA-9 mediated cardiac fibrosis in diabetes and its regulation by ZFAS1

et al. 2022

View full text Add to dashboard Cite

Diabetic cardiomyopathy (DCM) is one of the most prevalent causes of morbidity and mortality in diabetic patients. Hyperglycemia induces increased expression/deposition of extracellular matrix (ECM) proteins including fibronectin (FN) and collagen (Col) and plays an important role in fibrosis in diabetic cardiomyopathy (DCM). The roles of RNAs including microRNA (miRNA) and long non-coding RNAs (lncRNA) have begun to be understood in many conditions. In this study, we investigated the role of a specific miRNA, miR-9, and its interactions with lncRNA ZFAS1 in mediating fibrosis in DCM. Treatment with 25 mM glucose (HG) decreased miR-9 expression and increased expressions of ZFAS1, ECM proteins and inflammatory markers, compared to 5 mM glucose (NG) in the HCMECs by using qRT-PCR. Glucose-induced upregulation of ECM proteins can be prevented by ZFAS1 siRNA or miR-9 mimic transfection. Luciferase assay was confirmed miR-9 binding to FN 3’-UTR. miR-9 expression can be regulated by ZFAS1 through polycomb repressive complex 2 (PRC2) components using RNA immunoprecipitation (RIP) and chromatin immunoprecipitation (ChIP) assays. In the in vivo experiment, hyperglycemia-induced the ECM production can be prevented by the miR-9 overexpression in the fibrosis in DCM. These studies showed a novel glucose-induced molecular mechanism in which ZFAS1 participates in the transcriptional regulation of ECM protein production in diabetes through miR-9.

show abstract

Section: Introductionmentioning

confidence: 99%

Endothelial derived miRNA-9 mediated cardiac fibrosis in diabetes and its regulation by ZFAS1

et al. 2022

View full text Add to dashboard Cite

show abstract

“…The methylation of mi-RNA-9-1 was found to increase the chance of contracting diabetic retinopathy disease by five-fold, and both methylations of mi-RNA-9-1 and mi-RNA-9-3 increased this chance by eight-fold [139]. Additionally, downregulation of mi-RNA-29b is promoted via Dnmt3b, and its overexpression is found to prevent osteoarthritis through the Dnmt3b/mi-RNA-29b/PTHLH/CDK4/RUNX2 axis, thus suppressing chondrocyte apoptosis and extracellular matrix degradation [140].…”

Section: Epigenetic Impactmentioning

confidence: 96%

Exosomes and Micro-RNAs in Aging Process

2021

View full text Add to dashboard Cite

Exosomes are the main actors of intercellular communications and have gained great interest in the new cell-free regenerative medicine. These nanoparticles are secreted by almost all cell types and contain lipids, cytokines, growth factors, messenger RNA, and different non-coding RNA, especially micro-RNAs (mi-RNAs). Exosomes’ cargo is released in the neighboring microenvironment but is also expected to act on distant tissues or organs. Different biological processes such as cell development, growth and repair, senescence, migration, immunomodulation, and aging, among others, are mediated by exosomes and principally exosome-derived mi-RNAs. Moreover, their therapeutic potential has been proved and reinforced by their use as biomarkers for disease diagnostics and progression. Evidence has increasingly shown that exosome-derived mi-RNAs are key regulators of age-related diseases, and their involvement in longevity is becoming a promising issue. For instance, mi-RNAs such as mi-RNA-21, mi-RNA-29, and mi-RNA-34 modulate tissue functionality and regeneration by targeting different tissues and involving different pathways but might also interfere with long life expectancy. Human mi-RNAs profiling is effectively related to the biological fluids that are reported differently between young and old individuals. However, their underlying mechanisms modulating cell senescence and aging are still not fully understood, and little was reported on the involvement of mi-RNAs in cell or tissue longevity. In this review, we summarize exosome biogenesis and mi-RNA synthesis and loading mechanism into exosomes’ cargo. Additionally, we highlight the molecular mechanisms of exosomes and exosome-derived mi-RNA regulation in the different aging processes.

show abstract