Methylation Statuses of H19DMR and KvDMR at WT2 in Wilms Tumors in Taiwan

Lu, Meng‐Yao; Wang, Wen Chung; Hou, Tai-Cheng; Kuo, Chen‐Yun; Lai, Yen‐Chein

doi:10.1007/s12253-020-00802-6

Cited by 3 publications

(13 citation statements)

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“…ME001 can simultaneously detect changes in the degree of methylation of up to 24 different genes in a single reaction tube. Due to MLPA's easy operation, low cost, and wide range of applications, our team has published several studies based on this method [13,15,16]. However, MLPA analysis of tumor samples only provides information regarding the "average" situation in the cells from which the DNA samples were puri ed [14].…”

Section: Discussionmentioning

confidence: 99%

“…Genomic DNA was puri ed from para n-embedded tissues with the DNA FFPE Tissue Kit (Qiagen), according to the manufacturer's instructions and dissolved in 100 µl of TE buffer (10 mM Tris-HCl, pH 8.0, and 1 mM EDTA) as previously described [11,13]. UV-Vis measurements of DNA concentration of each sample were obtained using NanoDrop UV-VIS Spectrophotometer (Thermo Scienti c Nano-Drop 2000c).…”

Section: Dna Extractionmentioning

confidence: 99%

“…The six subjects have been described elsewhere [11][12][13]. Their para n-embedded Wilms tumor tissue samples were provided by the Department of Pediatrics of National Taiwan University Hospital.…”

Section: Study Subjectsmentioning

confidence: 99%

“…Then, sections were stained with hematoxylin and eosin and reviewed by two of the authors (T-C Hou and C-Y Kuo) under a microscope to con rm their diagnostic classi cations. Typing of Wilms tumor has been described in detail elsewhere [11,13]. W7 is of blastemal type and W8 is of epithelial type.…”

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confidence: 99%

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Identification of Molecular Biomarkers in Taiwanese Patients with Wilms Tumor Using a Methylation-Specific Multiplex Ligation-Dependent Probe Amplification (MS-MLPA)-Based Approach

Wang

Hou

et al. 2020

Preprint

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Background: Wilms tumor is a solid tumor that frequently occurs in children. Genetic or epigenetic aberrations in WT1 and WT2 loci are implicated in its etiology. Moreover, tumor suppressor genes are frequently silenced by methylation in this tumor. Methods: In the present study, we analyzed the methylation statuses of promoter regions of 24 different tumor suppressor genes using a methylation-specific multiplex ligation-dependent probe amplification (MS-MLPA)-based approach in six Wilms tumors. Results: All six Wilms tumors showed methylation of RASSF1 specific to tumors, not in normal tissues. Moreover, methylated HIC1 was identified in stromal type Wilms tumors and methylated BRCA1 was identified in epithelial type Wilms tumors. Unmethylated CASP8, RARB, MLH1_167, APC, and CDKN2A were found only in blastemal predominant type Wilms tumors. Conclusions: Our results indicated that methylation of RASSF1 is the essential event in the tumorigenesis of Wilms tumor, which may inform its clinical and therapeutic management. In addition, mixed type Wilms tumors may be classified according to epithelial, stromal, and blastemal components via MS-MLPA-based approach.

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Section: Discussionmentioning

confidence: 99%

Section: Dna Extractionmentioning

confidence: 99%

Section: Study Subjectsmentioning

confidence: 99%

mentioning

confidence: 99%

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Identification of Molecular Biomarkers in Taiwanese Patients with Wilms Tumor Using a Methylation-Specific Multiplex Ligation-Dependent Probe Amplification (MS-MLPA)-Based Approach

Wang

Hou

et al. 2020

Preprint

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“…The 6 subjects have been described elsewhere 11–13 . Their paraffin‐embedded Wilms' tumour tissue samples were provided by the Department of Pediatrics of National Taiwan University Hospital.…”

Section: Methodsmentioning

confidence: 99%

Correlations between histological characterizations and methylation statuses of tumour suppressor genes in Wilms' tumours

Lai

Wang

et al. 2022

Int J Experimental Path

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Wilms' tumour, also known as nephroblastoma, is an embryonal malignant tumour of the kidney. It is the most commonly occurring solid tumour in children, excluding brain tumours. [1][2][3] The aetiology of Wilms' tumour is still poorly understood, although a number of associated genes and loci have been identified. 4,5 The genetic changes in Wilms' tumours are diverse and involve approximately 40 genes. 6 Genes that have been previously implicated include WT1,

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The probable role of retroelements in the development of Wilms’ tumor in chromosomal syndromes

Мустафин

2023

Onkourologiâ

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The review article analyzes the data accumulated in the literature on the association of Wilms’ tumor with chromosomal syndromes and searches for possible causes of this phenomenon. In 10 % of all cases, nephroblastoma is represented by a hereditary tumor syndrome due to germline mutations in suppressor genes, mainly in the WT1 gene, less often in WT2, WTX, CTNNB1, TP53. These genes are associated with retroelements that play a role in the development of Wilms’ tumor, promoting carcinogenesis, causing genome instability. LINE-1 retroelement is a negative regulator of WT1 expression, while suppressor genes are characterized by suppression of retroelement activity. Part of the pathogenesis of Perlman, Beckwith-Wiedemann, WAGR, and trisomy 18 syndromes caused by germline microdeletions is the activation of retroelements that promote somatic chromosomal rearrangements, including deletions, insertions, and translocations, which are characteristic of sporadic Wilms’ tumor. Long noncoding RNAs and microRNAs are formed from retroelements during evolution or directly during the processing of their transcripts. At the same time, long noncoding RNAs affect the development of Wilms’ tumor by various mechanisms: due to the effect on ferroptosis (lncRNA AC007406.1, AC005208.1, LINC01770, DLGAP1-AS2, AP002761.4, STPG3-AS1, AC129507.1, AC234772.2, LINC02447, AC009570.1, ZBTB20-AS1 and LINC01179), Wnt/β-catenin signaling pathways (HOTAIR, MEG3), apoptosis (HAGLROS), regulation of expression of specific miRNAs (SNHG6, MEG8, XIST, SNHG16, DLEU1, CRNDE, SNHG6, DLGAP1, OSTM1-AS1, EMX2OS, H19). Analysis of the MDTE DB database revealed nephroblastoma-associated miRNAs that originate from retrotransposons. These include miR-192, -335, -378c, -562, -630, -1248. These molecules are promising for possible use in the pathogenetic treatment of Wilms’ tumor due to their effect on pathologically activated retrotransposons.

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Methylation Statuses of H19DMR and KvDMR at WT2 in Wilms Tumors in Taiwan

Cited by 3 publications

References 20 publications

Identification of Molecular Biomarkers in Taiwanese Patients with Wilms Tumor Using a Methylation-Specific Multiplex Ligation-Dependent Probe Amplification (MS-MLPA)-Based Approach

Identification of Molecular Biomarkers in Taiwanese Patients with Wilms Tumor Using a Methylation-Specific Multiplex Ligation-Dependent Probe Amplification (MS-MLPA)-Based Approach

Correlations between histological characterizations and methylation statuses of tumour suppressor genes in Wilms' tumours

The probable role of retroelements in the development of Wilms’ tumor in chromosomal syndromes

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