Methylene-blue/Coriphosphine-O/Acridine-Orange, chromosomal DNA and visible light: Interaction and cytological effect in Drosophila melanogaster

Mezzanotte, R.; Ferrucci, Luigi; Marchi, Annalisa

doi:10.1007/bf00127203

Cited by 4 publications

(2 citation statements)

References 25 publications

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“…This corresponds well with the high amount of pectin (mainly rhamnogalaturonans) present in soybeans (Huisman et al, 1998(Huisman et al, , 2003. Besides being used as a stain for detection of pectin in plant sources (Mosele et al, 2011), coriphosphine O is known to stain other cell components such as DNA and RNA (Mezzanotte et al, 1981) which can also be seen as labelling of nuclei in Figure 2B. Alcian blue is a dye with high affinity for pectic substances (Gunawardena et al, 2007;Arend et al, 2008) and was used to support the results found with coriphosphine O staining showing pectin solubilisation.…”

Section: Discussionmentioning

confidence: 54%

Enzymatic Solubilisation and Degradation of Soybean Fibre Demonstrated by Viscosity, Fibre Analysis and Microscopy

Ravn¹,

Martens²,

Pettersson³

et al. 2015

JAS

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The effect of a commercial multienzyme product obtained by fermentation from Aspergillus aculeatus on soybean and soybean meal was investigated using viscosity measurements, dietary fibre component analysis and different microscopy techniques utilizing histochemical dyes and antibody labelling. The results obtained demonstrated a strong viscosity reducing effect of the enzyme preparation on soluble galactomannan and xyloglucan polysaccharides and in addition non-starch polysaccharide analysis demonstrated a notable solubilisation of all polysaccharide constituents. The degradation of these components as native integral parts of cell walls upon exposure to the enzyme was visualized with microscopy. Two histochemical dyes, coriphosphine O and alcian blue were successfully used to follow pectin solubilisation after enzyme treatment. Commercial antibodies recognizing specific components of pectin and hemicellulose components of soybean cell wall were also used to visualize several enzyme activities in the commercial enzyme preparation The challenges of using commercial antibodies elicited from a given plant source to detect similar epitiopes on another plant source are also discussed. Non-starch polysaccharide analysis of the insoluble dietary fibre constituents before and after enzyme treatment corroborated the visualized mode of action demonstrated by microscopy. The combination of techniques provided visual and quantitative measurements of the solubilisation and degradation of hemicellulose pectic soybean cell wall components as part of the undesirable antinutrients in animal feed.

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Section: Discussionmentioning

confidence: 54%

Enzymatic Solubilisation and Degradation of Soybean Fibre Demonstrated by Viscosity, Fibre Analysis and Microscopy

Ravn¹,

Martens²,

Pettersson³

et al. 2015

JAS

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“…Acridine orange (Cambier, Wheeless & Patten, 1977; Fukuda et al. , 1979, 1986; Mezzanotte, Ferrucci & Marchi, 1981; Miyoshi & Fukuda, 1986), aminoacridines (Blake & Peacocke, 1968), acriflavine (Roth, 1976) or proflavine (Sagawa & Tatsumi, 1997) can be used to detect DNA by fluorescence. Acridine orange, acridine yellow or choriphosphine O are also used to detect RNA by metachromatic fluorescence (Bradley & Wolf, 1959).…”

Section: Discussionmentioning

confidence: 99%

Determination of red cells, nucleic acid‐containing cells and platelets (RNP Determination) by a crossover analysis of emission DNA/RNA light

Nagai

Kondo

Yamamoto

et al. 2009

Int J Lab Hematology

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We developed a new flow cytometry analysis method using a crossover analysis of emission light from intracellular DNA/RNA. Both the RNA and the DNA content in each cell were new parameters obtained by fluorescence analysis using acridine orange supravital stain. With this method, two-dimensional diagrams produced by cellular RNA concentration (CRc) and cellular DNA concentration (CDc) enabled clear separation of red cells and platelets, and the diagram (RNP Diagram) also distinguished fluorescently stained blood cells and small particles derived from background dust of the reagent. This study assessed the capability of RNP Determination concerning reticulocyte count and accurate platelet count obtained by the ratio of red cells and platelets. The distance of each event between red cell distribution and platelet distribution was sufficiently large on the diagram, and the two regions did not overlap. Both reticulocyte count and platelet count showed excellent correlation with those obtained by their respective reference methods. In conclusion, this new assay, using RNP Determination, demonstrated great potential for detecting abnormalities of red cells and platelets.

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Further evidence for the DNA base specificity of light-induced banding

Ferruchhi

Sumner

1984

Histochem J

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Experiments have been carried out to investigate the DNA base specificity of light-induced banding (LIB) produced by photo-oxidation of chromosomes followed by Acridine Orange staining to detect denatured DNA. Nuclei of different base composition, human and onion, and fluorochromes of different base specificities and modes of binding to DNA were used. Our results indicate that specific destruction of guanine residues is the main effect of photo-oxidation under the conditions used, and that LIB is a base-specific phenomenon. In addition, photo-oxidation may also cause DNA-protein cross-linking which affects the binding of some dyes, while prolonged photo-oxidation appears to cause more general damage to DNA.

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Methylene-blue/Coriphosphine-O/Acridine-Orange, chromosomal DNA and visible light: Interaction and cytological effect in Drosophila melanogaster

Cited by 4 publications

References 25 publications

Enzymatic Solubilisation and Degradation of Soybean Fibre Demonstrated by Viscosity, Fibre Analysis and Microscopy

Enzymatic Solubilisation and Degradation of Soybean Fibre Demonstrated by Viscosity, Fibre Analysis and Microscopy

Determination of red cells, nucleic acid‐containing cells and platelets (RNP Determination) by a crossover analysis of emission DNA/RNA light

Further evidence for the DNA base specificity of light-induced banding

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