Methylobacterium hispanicum sp. nov. and Methylobacterium aquaticum sp. nov., isolated from drinking water

Gallego, Virginia; Garcı́a, Marı́a Teresa; Ventosa, António

doi:10.1099/ijs.0.63319-0

Cited by 81 publications

(58 citation statements)

References 35 publications

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“…The LAII-25 clone sequence showed close similarity (99.7%) to Methylobacterium variabile (AJ851087), a pink pigmented facultatively methylotrophic (PPFM) alphaproteobacterium [35]. Cyanobacterial mats are known to support the growth of such organisms by the production of organic compounds like dimethyl sulfide (DMS), dimethyl trisulfide, alcohols, esters and aldehydes [36].…”

Section: Mat Associated Diversity Of Pre-monsoon Seasonmentioning

confidence: 99%

Temporal bacterial diversity and detection of putative methanotrophs in surface mats of Lonar crater lake

Surakasi¹,

Antony²,

Sharma³

et al. 2010

J. Basic Microbiol.

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The phylogenetic diversity of bacterial communities in microbial mats of two different seasons from saline and hyperalkaline Lonar Lake was investigated using 16S rRNA gene library analysis. Arthrospira (Cyanobacteria) related clones (>80% of total clones) dominated libraries of both the seasons. Clear differences were found in both the seasons as the operational taxonomic units (OTUs) related to Fusibacter (LAI-1 and LAI-59) and Tindallia magadiensis found in post-monsoon were not found in the pre-monsoon library. Likewise, OTUs related to Planococcus rifietensis (LAII-67), Bordetella hinzii (LAII-2) and Methylobacterium variabile found in the pre-monsoon were not found in post-monsoon. The study was extended to identify methanotrophs in the surface mats. Libraries constructed with type I and type II methanotroph specific 16S rRNA gene primers showed the presence of clones (LAMI-99 and LAMII-2) closely related to Methylomicrobium buryaticum and Beijerinckiaceae family members. Denaturing gradient gel electrophoresis (DGGE) fingerprinting based on protein-coding genes (pmoA and mxaF) further confirmed the detection of Methylomicrobium sp. Hence, we report here for the first time the detection of putative methanotrophs in surface mats of Lonar Lake. The finding of clones related to organisms with interesting functional attributes such as assimilation of C 1 compounds (LAII-25, LAMI-39, LAMI-99 and LAMII-2), non-sulfur photosynthetic bacteria (LAMII-43) and clones distantly affiliated to organisms of heavily polluted environments (LAI-59 and LAMII-52), is of significant note. These preliminary results would direct future studies on the functional dynamics of microbial mat associated food web chain in the extreme environment.

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Section: Mat Associated Diversity Of Pre-monsoon Seasonmentioning

confidence: 99%

Temporal bacterial diversity and detection of putative methanotrophs in surface mats of Lonar crater lake

Surakasi¹,

Antony²,

Sharma³

et al. 2010

J. Basic Microbiol.

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“…Strain PMB02 T is a slow-growing organism that does not grow in the presence of ¢1 % NaCl. M. aquaticum CCM 7218 T (Gallego et al, 2005a) and M. variabile GR3 T (Gallego et al, 2005c) also do not grow in the presence of ¢1 % NaCl, but M. lusitanum (Doronina et al, 2002) and M. suomiense (Doronina et al, 2002) can grow in the presence of 3 % NaCl (Table 1). To investigate whether strain PMB02 T produced polyhydroxyalkanoates, cells were grown in TYG medium for 72 h, then washed twice with PBS and transferred to modified MSB medium containing succinate (10 mM).…”

mentioning

confidence: 99%

“…The genus Methylobacterium was first described by Patt et al (1976) and at the time of writing consists of the following 24 recognized species (http://www.bacterio.cict.fr/m/methylobacterium. html): Methylobacterium adhaesivum (Gallego et al, 2006), M. aminovorans (Urakami et al, 1993), M. aquaticum (Gallego et al, 2005a), M. chloromethanicum (McDonald et al, 2001), M. dichloromethanicum (Doronina et al, 2000), M. extorquens (Bousfield & Green, 1985), M. fujisawaense (Green et al, 1988), M. hispanicum (Gallego et al, 2005a), M. isbiliense (Gallego et al, 2005c), M. jeotgali (Aslam et al, 2007), M. lusitanum (Doronina et al, 2002), M. mesophilicum , M. nodulans (Jourand et al, 2004), M. organophilum (Patt et al, 1976), M. oryzae (Madhaiyan et al, 2007), M. podarium (Anesti et al, 2004), M. populi (Van Aken et al, 2004), M. radiotolerans , M. rhodesianum (Green et al, 1988), M. rhodinum , M. suomiense (Doronina et al, 2002), M. thiocyanatum (Wood et al, 1998), M. variabile (Gallego et al, 2005b) and M. zatmanii (Green et al, 1988). Here, we provide a detailed taxonomic characterization of a Methylobacterium-like bacterial strain, PMB02 T , which was isolated from a tree leaf.…”

mentioning

confidence: 99%

Methylobacterium platani sp. nov., isolated from a leaf of the tree Platanus orientalis

Kang

Kim

Shin

et al. 2007

International Journal of Systematic and Evolutionary Microbiology

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A novel bacterial strain, designated PMB02T, was isolated from a leaf of the tree Platanus orientalis. Colonies grown on TYG agar plates were circular, pink-pigmented and slow-growing, being 0.2–1.5 mm in diameter after 3 days growth. The cells of strain PMB02T were Gram-negative, aerobic, motile rods that possessed oxidase and catalase activities and grew at 20–30 °C, pH 6–8 and in media containing less than 1 % NaCl. The major respiratory quinone was identified as Q-10. A phylogenetic analysis based on 16S rRNA gene sequence comparisons indicated that strain PMB02T was related to members of the genus Methylobacterium. A comparative 16S rRNA gene sequence-based phylogenetic analysis placed the strain in a clade with the species Methylobacterium aquaticum and Methylobacterium variabile, with which it showed sequence similarities of 97.7 and 97.4 %, respectively. The values for DNA–DNA hybridization between strain PMB02T and M. aquaticum CCM 7218T and M. variabile GR3T were less than 32 %. On the basis of the phenotypic characterization, the phylogenetic analysis and the DNA–DNA relatedness data, strain PMB02T is considered to represent a novel species of the genus Methylobacterium, for which the name Methylobacterium platani sp. nov. is proposed. The type strain is PMB02T (=KCTC 12901T=JCM 14648T).

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“…Strain AR27 T was characterized phenotypically by using the methods described by Doronina et al (1998). The use of different carbon compounds was determined as described previously by Gallego et al (2005a) by using Biolog Microplates (Biolog Inc.).Chromosomal DNA was isolated and purified according to the methods described by Wilson (1997) and a partially modified Marmur method (Marmur, 1961;Hood et al, 1987). The 16S rRNA gene was amplified using the universal primers 16F27 and 16R1488 as described by Mellado et al (1995).…”

mentioning

confidence: 99%

mentioning

confidence: 99%

Methylobacterium adhaesivum sp. nov., a methylotrophic bacterium isolated from drinking water

Gallego

Garcı́a

Ventosa

2006

International Journal of Systematic and Evolutionary Microbiology

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A pink-pigmented, facultatively methylotrophic strain, AR27 T , isolated from the drinking water distribution system of Seville, Spain, was characterized taxonomically. Cells were Gram-negative rods, motile, non-spore-forming and strictly aerobic. Growth in liquid media was flocculant whereas on solid media growth produced colonies that usually adhered to the agar surface. On the basis of its phenotypic characteristics, 16S rRNA gene sequence data and DNA G+C content (63?6 mol%), strain AR27 T (=CECT 7069 T =CCM 7305 T ) is proposed as the type strain of a novel species of the genus Methylobacterium, Methylobacterium adhaesivum sp. nov.The genus Methylobacterium belongs to the a-2 subclass of the Proteobacteria and has the serine pathway for formaldehyde assimilation. It includes strictly aerobic, Gram-negative, rod-shaped, pink-pigmented, facultatively methylotrophic (PPFM) bacteria, which can grow on single carbon compounds such as formate, formaldehyde and methanol as the sole source of carbon and energy, as well as on a wide range of multi-carbon growth substrates (Green, 2001). At the time of writing, the genus comprises 20 recognized species (Patt et al., 1976;Bousfield & Green, 1985;Green et al., 1988;Urakami et al., 1993;Wood et al., 1998; Doronina et al., 2000Doronina et al., , 2002 McDonald et al., 2001;Van Aken et al., 2004;Jourand et al., 2004; Gallego et al., 2005a, b, c), with Methylobacterium organophilum as the type species (Patt et al., 1976).Members of the genus Methylobacterium are ubiquitous in terrestrial habitats (Green & Bousfield, 1981, including soil, dust, freshwater, tap water systems, lake sediments, leaf surfaces and nodules, rice grains and air, and as contaminants in various products and processes. The PPFM bacteria are strict aerobes and can be isolated from almost any freshwater environment where some dissolved oxygen exists, e.g. from tap water systems (Gräf & Bauer, 1973) and from stratified lake systems (Hanson, 1980). Furthermore, species of this genus have been isolated from hospital environments (Gilardi & Faur, 1984), where they may pose a threat as opportunistic pathogens to seriously ill patients.A sampling campaign in April 2004 to identify culturable and non-culturable bacteria present in the drinking water distribution system of Seville, Spain, led to isolation of a slowly growing pink-pigmented bacterium. This isolate was studied phenotypically, phylogenetically and genotypically. Based on these characteristics we suggest that strain AR27 T represents a novel species of the genus Methylobacterium.Drinking water samples (25 l) were concentrated by using a tangential flow filtration system (Filtron), plated on plate count agar (PCA; Difco) and R2A (Difco) and incubated at 28 uC for 7 days. Distinct colonies were picked in order to obtain pure cultures. Pink-pigmented colonies were selected and studied in more detail. One of these strains, AR27 T , showed a characteristic growth that differentiated it from the remaining pink-pigmented colonies isolated. Strain AR27 ...

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Methylobacterium hispanicum sp. nov. and Methylobacterium aquaticum sp. nov., isolated from drinking water

Cited by 81 publications

References 35 publications

Temporal bacterial diversity and detection of putative methanotrophs in surface mats of Lonar crater lake

Temporal bacterial diversity and detection of putative methanotrophs in surface mats of Lonar crater lake

Methylobacterium platani sp. nov., isolated from a leaf of the tree Platanus orientalis

Methylobacterium adhaesivum sp. nov., a methylotrophic bacterium isolated from drinking water

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