Methylocella silvestris sp. nov., a novel methanotroph isolated from an acidic forest cambisol

Dunfield, Peter F.; Khmelenina, V. N.; Suzina, N. E.; Trotsenko, Yu. A.; Dedysh, Svetlana N.

doi:10.1099/ijs.0.02481-0

Cited by 216 publications

(180 citation statements)

References 27 publications

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“…Methylocella spp. lack the particulate methane monooxygenase encoded by the pmoCAB operon, which is present in all other methanotrophs (Dedysh et al, 2000(Dedysh et al, , 2004Dunfield et al, 2003). Although a few studies have detected Methylocella-related 16S rRNA genes in different environments (for example, Dunfield et al, 2003;Dedysh et al, 2000Dedysh et al, , 2004Chen et al, 2008a, b;Cockell et al, 2009;Fabiani et al, 2009;Han et al, 2009), there is little information on the distribution and abundance of Methylocella.…”

Section: Introductionmentioning

confidence: 99%

“…lack the particulate methane monooxygenase encoded by the pmoCAB operon, which is present in all other methanotrophs (Dedysh et al, 2000(Dedysh et al, , 2004Dunfield et al, 2003). Although a few studies have detected Methylocella-related 16S rRNA genes in different environments (for example, Dunfield et al, 2003;Dedysh et al, 2000Dedysh et al, , 2004Chen et al, 2008a, b;Cockell et al, 2009;Fabiani et al, 2009;Han et al, 2009), there is little information on the distribution and abundance of Methylocella. Current molecular techniques for example, pmoA microarray (Bodrossy et al, 2003) and real-time quantitative PCR (qPCR) (Kolb et al, 2003;Tuomivirta et al, 2009), that are commonly applied to study the diversity and abundance of methanotrophs, would not detect Methylocella, as these methods target pmoA.…”

Section: Introductionmentioning

confidence: 99%

“…are aerobic facultative methanotrophs (Alphaproteobacteria), with three validated species, M. palustris, M. silvestris and M. tundrae, isolated from acidic soils (Dunfield et al, 2003;Dedysh et al, 2000Dedysh et al, , 2004. M. silvestris BL2, which can grow on a variety of multicarbon substrates (Dedysh et al, 2005;Theisen et al, 2005), oxidizes methane using the soluble methane monooxygenase encoded by the operon mmoXYBZDC.…”

Section: Introductionmentioning

confidence: 99%

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Environmental distribution and abundance of the facultative methanotroph Methylocella

et al. 2010

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Methylocella spp. are facultative methanotrophs, which are able to grow not only on methane but also on multicarbon substrates such as acetate, pyruvate or malate. Methylocella spp. were previously thought to be restricted to acidic soils such as peatlands, in which they may have a key role in methane oxidation. There is little information on the abundance and distribution of Methylocella spp. in the environment. New primers were designed, and a real-time quantitative PCR method was developed and validated targeting Methylocella mmoX (encoding the a-subunit of the soluble methane monooxygenase) that allowed the quantification of Methylocella spp. in environmental samples. We also developed and validated specific PCR assays, which target 16S rRNA genes of known Methylocella spp. These were used to investigate the distribution of Methylocella spp. in a variety of environmental samples. It was revealed that Methylocella species are widely distributed in nature and not restricted to acidic environments.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Environmental distribution and abundance of the facultative methanotroph Methylocella

et al. 2010

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“…temperature, rainfall), have a considerable influence on the microbial (methanogenic and methanotrophic) activity in the peat (Ding et al 2004). It has been found that the optimal temperature for both methanogens and methanotrophs is 20-30°C (Dunfield et al, 2003;Le Mer and Roger 2001). In the current study, the influence of temperature was also visible, especially in the locations covered by C. nigra and V. oxycoccos where summer emissions were the highest (Fig.…”

Section: −1mentioning

confidence: 99%

Methane Oxidation by Endophytic Bacteria Inhabiting Sphagnum sp. and Some Vascular Plants

et al. 2018

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Methane emission from wetlands is responsible for about 24% of the total CH 4 emissions. The value of emission is a result of the balance between the processes of methane formation (methanogenesis) and sinks (methanotrophy). The methanotrophic activity from well-aerated soil surface layers has been relatively well recognized. On the contrary, the active role of plants in reduction of methane emission is rather not fully known. The association of methanotrophic bacteria with plants of Sphagnum spp., has already been recognized. In our investigations, particular attention was paid to vascular plants from a peatland overgrown by Sphagnum spp. but also Eriophorum vaginatum, Carex nigra, and Vaccinium oxycoccos. The gases emitted from the surface of Moszne peatland were collected using the chamber method from selected sites during growing seasons (spring, summer, autumn). To estimate the contribution of plants in methane emissions from the peatland, in each investigated site gas was sampled from the surface with the native flora cover and after removal thereof. Our results show that the reduction in the CH 4 emission was related to the plant composition, vegetation period, and conditions of the plants. It was confirmed that the endophytes under investigation belonged to type I methanotrophs.

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“…Either pMMO or sMMO exist in methanotrophs although some genera contain both. The majority of methanotrophs possesses a particulate methane monooxygenase enzyme whereas a few species contain only soluble methane monooxygenase enzyme e.g., Methylocella silvestris [6], [7] and Methyloferula stellata [8].…”

Section: Introductionmentioning

confidence: 99%

Physiological Features during the Copper Switch in Methylococcus Capsulatus (Bath)

Khalifa¹

2013

IJBBB

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Abstract-Copper-to-biomass ratio is an important factor that controls the biosynthesis and catalytic activity of the two forms (particulate or soluble) of methane monooxygenase enzyme in Methylococcus capsulatus (Bath). Under growth conditions of high copper-to-biomass ratio, the particulate enzyme is expressed while the soluble enzyme is up-regulated when this ratio is low. It is of interest to explore physiological features of cells in response to copper addition. To this end, M. capsulatus was grown on Nitrate Mineral Salts medium in a bioreactor and 30 µM copper was added at a dilution rate of 3.3 ml min -1 at steady state. Samples were collected at different time points and assayed for soluble methane monooxygenase (sMMO) expression using the naphthalene assay, SDS-PAGE of total cell protein and copper measurements. The results showed that the activity of sMMO was gradually decreased upon gradual copper addition until it became fully inactive after 4 hours of adding copper. Furthermore, the intracellular, extracellular and copper associated with biomass increased by adding copper over the time tested. Eleven bands from the SDS-PAGE gel of sMMO-expressing cells and pMMO-expressing cells, were analyzed by mass spectrometry. The results highlighted that 128 proteins were identified from 792 polypeptides detected from the eleven bands analysed. Typically, 506 polypeptides from sMMO-expressing cells (corresponding to 87 proteins) and 236 from pMMO-expressing cells (corresponding to 41) were identified. Collectively, the results confirmed that copper negatively regulates sMMO and induces pMMO in M. capsulatus.

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Methylocella silvestris sp. nov., a novel methanotroph isolated from an acidic forest cambisol

Cited by 216 publications

References 27 publications

Environmental distribution and abundance of the facultative methanotroph Methylocella

Environmental distribution and abundance of the facultative methanotroph Methylocella

Methane Oxidation by Endophytic Bacteria Inhabiting Sphagnum sp. and Some Vascular Plants

Physiological Features during the Copper Switch in Methylococcus Capsulatus (Bath)

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