Methylopila turkiensis sp. nov., a new aerobic facultatively methylotrophic phytosymbiont

Agafonova, N. V.; Капаруллина, Е. Н.; Доронина, Н. В.; Trotsenko, Yu. A.

doi:10.1134/s0026261715040025

Cited by 9 publications

(2 citation statements)

References 24 publications

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“…While these taxa comprised a negligible fraction of the Sphagnum microbiome under ambient conditions, they increased to relative abundances of 3%–6% in the warmest enclosures (Figure S17). Although these taxa are affiliated with known methanotrophs, they display the highest sequence identity to cultivated representatives of methylotrophs, which are capable of growth on methanol and other substrates rather than CH 4 (Agafonova et al, 2015; Berestovskaya et al, 2012; Doronina et al, 1998; Li et al, 2011). This is particularly surprising for the Methylopilaceae, whose relative abundance was significantly correlated to the rise in CH 4 oxidation rates (Figure S19).…”

Section: Discussionmentioning

confidence: 99%

Climate drivers alter nitrogen availability in surface peat and decouple N₂ fixation from CH₄ oxidation in the Sphagnum moss microbiome

Petro

Carrell

Wilson

et al. 2023

Global Change Biology

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Peat mosses (Sphagnum spp.) are keystone species in boreal peatlands, where they dominate net primary productivity and facilitate the accumulation of carbon in thick peat deposits. Sphagnum mosses harbor a diverse assemblage of microbial partners, including N2‐fixing (diazotrophic) and CH4‐oxidizing (methanotrophic) taxa that support ecosystem function by regulating transformations of carbon and nitrogen. Here, we investigate the response of the Sphagnum phytobiome (plant + constituent microbiome + environment) to a gradient of experimental warming (+0°C to +9°C) and elevated CO2 (+500 ppm) in an ombrotrophic peatland in northern Minnesota (USA). By tracking changes in carbon (CH4, CO2) and nitrogen (NH4‐N) cycling from the belowground environment up to Sphagnum and its associated microbiome, we identified a series of cascading impacts to the Sphagnum phytobiome triggered by warming and elevated CO2. Under ambient CO2, warming increased plant‐available NH4‐N in surface peat, excess N accumulated in Sphagnum tissue, and N2 fixation activity decreased. Elevated CO2 offset the effects of warming, disrupting the accumulation of N in peat and Sphagnum tissue. Methane concentrations in porewater increased with warming irrespective of CO2 treatment, resulting in a ~10× rise in methanotrophic activity within Sphagnum from the +9°C enclosures. Warming's divergent impacts on diazotrophy and methanotrophy caused these processes to become decoupled at warmer temperatures, as evidenced by declining rates of methane‐induced N2 fixation and significant losses of keystone microbial taxa. In addition to changes in the Sphagnum microbiome, we observed ~94% mortality of Sphagnum between the +0°C and +9°C treatments, possibly due to the interactive effects of warming on N‐availability and competition from vascular plant species. Collectively, these results highlight the vulnerability of the Sphagnum phytobiome to rising temperatures and atmospheric CO2 concentrations, with significant implications for carbon and nitrogen cycling in boreal peatlands.

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Section: Discussionmentioning

confidence: 99%

Climate drivers alter nitrogen availability in surface peat and decouple N₂ fixation from CH₄ oxidation in the Sphagnum moss microbiome

Petro

Carrell

Wilson

et al. 2023

Global Change Biology

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“…Methylopila carotae sp (KAPARULLINA et al, 2019),. Methylopila turkiensis sp (AGAFONOVA et al, 2015). Methylopila henanense sp (WANG et al, 2015)…”

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confidence: 99%

Produção de copolímeros do tipo P(3HB-co-3HV) por >i<Methylopila >/i<>i<oligotropha>/i< (cepa MCSUBH) isolada do manguezal de Cubatão-SP

Silva

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Produção de copolímeros do tipo P(3HB-co-3HV) por Methylopila oligotropha (cepa MCSUBH) isolada do manguezal de Cubatão-SP.São Paulo P(3HB-co-3HV) apenas quando o ácido valérico foi fornecido como co-substrato na presença de metanol. Além disso, foram testadas diferentes estratégias para a produção do copolímero como: tempo de cultivo, concentração de metanol e concentração de nitrogênio. A produção de P(3HB-co-3HV) variou entre 0,49 e 2,41g.L -1 com diferentes frações molares de ácido 3hidroxivalérico (3HV) (entre 1,86-20 mol%)Palavras chaves: Copolímeros. Poli(3-hidroxibutirato-co-3-hidroxivalerato). Metilotrófica.Metanol. Ácido Valérico.

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Methylopila

Agafonova,

Kaparullina,

Doronina

2024

Bergey's Manual of Systematics of Archaea and Bacteria

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Me.thy.lo.pi'la. N.L. neut. n. methyl , the methyl group; L. fem. n. pila , ball or sphere; N.L. Methylopila , methyl‐using sphere. Pseudomonadota / Alphaproteobacteria / Rhizobiales / Methylopilaceae / Methylopila The genus Methylopila accommodates strictly aerobic facultatively methylotrophic bacteria. Cells are Gram‐stain‐negative, rod shaped, asporogenous, and motile or nonmotile; occur singly or in pairs; multiply by binary fission; and contain poly‐β‐hydroxybutyrate granules. Utilize methanol and methylamine as well as a variety of multicarbon compounds as carbon and energy sources. Assimilate C 1 compounds by isocitrate lyase‐negative variant of the serine pathway. Growth factors are not required. Ammonium salts, nitrate, urea, peptone, some amino acids, and methylated amines are utilized as nitrogen sources. Do not grow on CH 4 or H 2 /CO 2 /O 2 . Oxidase‐, catalase‐, and urease‐positive. Indole is formed from l ‐tryptophan on mineral medium with methanol as the sole carbon and energy source and KNO 3 as the sole nitrogen source. Acids are produced from sugars oxidatively. Growth occurs at 10–37°C (but not at 42°C), pH 5.0–10.0. The major fatty acid is C 18:1 ω7 c . The major quinone is Q‐10. The genome sizes are 3.72–4.14 Mb. According to Genome Taxonomy Database (GTDB), the genus belongs to the phylum Pseudomonadota , class Alphaproteobacteria , order Rhizobiales , and family Methylopilaceae . Representatives of this genus inhabit phyllosphere and rhizosphere of various plants, fruits, and activated sludge, polluted and natural soils. DNA G + C content (mol%) : 64.5–70.4 ( T m and genome analyses). Type species : Methylopila capsulata Doronina et al. 1998 VP .

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Methylopila turkiensis sp. nov., a new aerobic facultatively methylotrophic phytosymbiont

Cited by 9 publications

References 24 publications

Climate drivers alter nitrogen availability in surface peat and decouple N₂ fixation from CH₄ oxidation in the Sphagnum moss microbiome

Climate drivers alter nitrogen availability in surface peat and decouple N₂ fixation from CH₄ oxidation in the Sphagnum moss microbiome

Produção de copolímeros do tipo P(3HB-co-3HV) por >i<Methylopila >/i<>i<oligotropha>/i< (cepa MCSUBH) isolada do manguezal de Cubatão-SP

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Methylopila turkiensis sp. nov., a new aerobic facultatively methylotrophic phytosymbiont

Cited by 9 publications

References 24 publications

Climate drivers alter nitrogen availability in surface peat and decouple N2 fixation from CH4 oxidation in the Sphagnum moss microbiome

Climate drivers alter nitrogen availability in surface peat and decouple N2 fixation from CH4 oxidation in the Sphagnum moss microbiome

Produção de copolímeros do tipo P(3HB-co-3HV) por >i<Methylopila >/i<>i<oligotropha>/i< (cepa MCSUBH) isolada do manguezal de Cubatão-SP

Methylopila

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Climate drivers alter nitrogen availability in surface peat and decouple N₂ fixation from CH₄ oxidation in the Sphagnum moss microbiome

Climate drivers alter nitrogen availability in surface peat and decouple N₂ fixation from CH₄ oxidation in the Sphagnum moss microbiome