A Gram-negative, facultatively anaerobic, chemo-organotrophic, non-pigmented, slow-growing bacterium was isolated from acidic Sphagnum peat and designated strain TPB6017T. Cells of this strain were long rods that multiplied by normal cell division and were motile by means of a single flagellum. Cells grew under reduced oxygen tension and under anoxic conditions and were able to ferment sugars and several polysaccharides, including amorphous and crystalline cellulose. Strain TPB6017T was a psychrotolerant acidophile capable of growth between pH 3.0 and 7.5 (optimum 4.5–5.0) and at 4–35 °C (optimum 20–28 °C). It was extremely sensitive to salt stress; growth was inhibited at NaCl concentrations above 0.1 % (w/v). The major fatty acids were iso-C15 : 0 and iso-C17 : 1ω9c; the polar lipids were phosphatidylethanolamine and a number of phospholipids and aminophospholipids with an unknown structure. The quinone was MK-8. The DNA G+C content was 57.6 mol%. Comparative 16S rRNA gene sequence analysis revealed that strain TPB6017T was a member of subdivision 1 of the phylum Acidobacteria and belonged to a phylogenetic lineage defined by the acidophilic aerobic chemo-organotroph Acidobacterium capsulatum (92.3 % sequence similarity). However, cell morphology, type of flagellation, the absence of pigment, differences in fatty acid and polar lipid composition, possession of a cellulolytic capability, inability to grow under fully oxic conditions and good growth in anoxic conditions distinguished strain TPB6017T from A. capsulatum. Therefore, it is proposed that strain TPB6017T represents a novel acidobacterium species in a new genus, Telmatobacter bradus gen. nov., sp. nov.; strain TPB6017T ( = DSM 23630T = VKM B-2570T) is the type strain.

No abstract

Two previously isolated strains (DSM 9103 T and LPM-4 T ) able to grow with EDTA (facultatively and obligately, respectively) as the source of carbon, nitrogen and energy were investigated in order to clarify their taxonomic positions. The strains were strictly aerobic, Gram-negative, asporogenous and non-motile rods that required biotin for growth. Reproduction occurred by binary fission. The strains were mesophilic and neutrophilic. Their major fatty acids were summed feature 7 (consisting of C 18 : 1 v7c, C 18 : 1 v9t and/or C 18 : 1 v12t) and C 19 : 0 cyclo v8c. The polyamine pattern revealed homospermidine as a major polyamine. Predominant polar lipids were phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, phosphatidyldimethylethanolamine, phosphatidylmonomethylethanolamine and diphosphatidylglycerol. Mesorhizobium-specific ornithine lipid was absent. The predominant isoprenoid quinone was Q-10. The DNA G+C values were 60.8 and 63.1 mol% (T m ) for strains LPM-4 T and DSM 9103 T , respectively. The level of 16S rRNA gene sequence similarity between these EDTA-utilizers was 99.3 % while the DNA-DNA hybridization value was only 37 %. Both strains were phylogenetically related to members of the genera Aminobacter and Mesorhizobium (95-97 % sequence similarity). However, DNA-DNA hybridization values between the novel EDTAdegrading strains and Aminobacter aminovorans DSM 7048 T and Mesorhizobium loti DSM 2626 T were low (10-11 %). Based on their genomic and phenotypic properties, the new alphaproteobacterial strains are assigned to a novel genus, Chelativorans gen. nov., with the names Chelativorans multitrophicus sp. nov. (type strain DSM 9103 T 5VKM B-2394 T ) and Chelativorans oligotrophicus sp. nov. (type strain LPM-4 T 5VKM B-2395 T 5DSM 19276 T ).EDTA is a chelating agent from the group of aminopolycarboxylic acids that are able to form stable, water-soluble complexes with many metal ions. At present, the amount of EDTA utilized worldwide amounts to some 103 000 tonnes per year. It is used in many diverse fields including the photographic and galvanic industries, in textile and paper manufacturing, for decontamination of nuclear power installations, as a component of industrial cleaners, as an additive in cosmetics and food products, in gas scrubbing for the removal of hydrogen sulfide from waste gases and in agricultural applications to improve the uptake of micronutrients to correct trace metal deficiencies in plants (Potthoff-Karl et al., 1996;Weilenmann et al., 2004). The predominantly water-based use of EDTA and the recalcitrance of EDTA to biodegradation have resulted in high concentrations of this agent in surface waters. The extensive environmental EDTA pollution that has been observed in Abbreviations: APL, aminophospholipid; DPG, diphosphatidylglycerol; PC, phosphatidylcholine; PDE, phosphatidyldimethylethanolamine; PE, phosphatidylethanolamine; PG, phosphatidylglycerol; PHB, poly-bhydroxybutyrate; PL, phospholipids; PME, phosphatidylmonomethylethanolamine.The GenBank/EMBL/DDBJ...

No abstract