Chelativorans multitrophicus gen. nov., sp. nov. and Chelativorans oligotrophicus sp. nov., aerobic EDTA-degrading bacteria

Доронина, Н. В.; Капаруллина, Е. Н.; Trotsenko, Yu. A.; Nörtemann, Bernd; Bucheli‐Witschel, Margarete; Weilenmann, Hans Ulrich; Egli, Thomas

doi:10.1099/ijs.0.003152-0

Cited by 50 publications

(30 citation statements)

References 43 publications

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“…When added to the supernatant, the amount of cations available is higher than in sterilized distilled water, and the EDTA free to chelate cations from membranes will be less, lowering its toxicity. On the other hand, the possibility of the strain to produce EDTA degrading proteins to the supernatant cannot be overruled [24]. …”

Section: Resultsmentioning

confidence: 99%

Nematicidal Bacteria Associated to Pinewood Nematode Produce Extracellular Proteases

et al. 2013

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Bacteria associated with the nematode Bursaphelenchus xylophilus, a pathogen of trees and the causal agent of pine wilt disease (PWD) may play a role in the disease. In order to evaluate their role (positive or negative to the tree), strains isolated from the track of nematodes from infected Pinus pinaster trees were screened, in vitro, for their nematicidal potential. The bacterial products, from strains more active in killing nematodes, were screened in order to identify and characterize the nematicidal agent. Forty-seven strains were tested and, of these, 21 strains showed capacity to produce extracellular products with nematicidal activity. All Burkholderia strains were non-toxic. In contrast, all Serratia strains except one exhibited high toxicity. Nematodes incubated with Serratia strains showed, by SEM observation, deposits of bacteria on the nematode cuticle. The most nematicidal strain, Serratia sp. A88copa13, produced proteases in the supernatant. The use of selective inhibitors revealed that a serine protease with 70 kDa was majorly responsible for the toxicity of the supernatant. This extracellular serine protease is different phylogenetically, in size and biochemically from previously described proteases. Nematicidal assays revealed differences in nematicidal activity of the proteases to different species of Bursaphelenchus, suggesting its usefulness in a primary screen of the nematodes. This study offers the basis for further investigation of PWD and brings new insights on the role bacteria play in the defense of pine trees against B. xylophilus. Understanding all the factors involved is important in order to develop strategies to control B. xylophilus dispersion.

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Section: Resultsmentioning

confidence: 99%

Nematicidal Bacteria Associated to Pinewood Nematode Produce Extracellular Proteases

et al. 2013

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“…Phyllobacteriaceae representatives have been characterized to produce Q-10 as the predominant (Doronina et al 2010;Jung et al 2012;Peix et al 2005;Roh et al 2008;Urakami et al 1992;Yabe et al 2012) or sole respiratory quinone (Stevenson et al 2011). Phyllobacteriaceae representatives have been characterized to produce Q-10 as the predominant (Doronina et al 2010;Jung et al 2012;Peix et al 2005;Roh et al 2008;Urakami et al 1992;Yabe et al 2012) or sole respiratory quinone (Stevenson et al 2011).…”

Section: %) Andmentioning

confidence: 99%

“…The type species of the genera Phyllobacterium, Aquamicrobium, Nitratireductor, Pseudahrensia and Thermovum exhibit nitrate reduction (Mergaert et al 2002;Bambauer et al 1998;Labbé et al 2004;Yabe et al 2012;Jung et al 2012), Aquamicrobium can metabolize sulfonated aromatic compounds (Bambauer et al 1998), whereas Chelativorans, Aminobacter and Pseudaminobacter can degrade EDTA, methylamine and salicylate, respectively (Doronina et al 2010;Urakami et al 1992;Kämpfer et al 1999). On the other hand, the type species of the genera Mesorhizobium and Phyllobacterium are plantassociated, in which Mesorhizobium loti can even nodulate leguminous plants (Jarvis et al 1997;Knösel 1984;Mergaert et al 2002).…”

Section: Introductionmentioning

confidence: 99%

Oricola cellulosilytica gen. nov., sp. nov., a cellulose-degrading bacterium of the family Phyllobacteriaceae isolated from surface seashore water, and emended descriptions of Mesorhizobium loti and Phyllobacterium myrsinacearum

Hameed

Shahina

Lai

et al. 2015

Antonie van Leeuwenhoek

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A Gram-stain negative, strictly aerobic, rod-shaped, non-spore-forming, motile cellulolytic bacterium, designated strain CC-AMH-0(T), was isolated from surface seashore water of Hualien, Taiwan and subjected to polyphasic taxonomy. Strain CC-AMH-0(T) exhibited enzymatic saccharification of cellulose and active growth particularly during log-phase under nutrient-limited conditions, whereas enhanced saccharification was found in the declining growth phase under copiotrophic conditions. The novel strain shared high pairwise 16S rRNA gene sequence similarities to Mesorhizobium loti USDA 3471(T) (96.2 %), Phyllobacterium myrsinacearum IAM 13584(T) (95.9 %), Hoeflea marina LMG 128(T) (94.0 %) and other Phyllobacteriaceae members. However, phylogenetic analyses based on 16S rRNA, atpD and recA gene sequences clearly distinguished strain CC-AMH-0(T) from other representatives of related genera. In addition, strain CC-AMH-0(T) was distinguished from the above mentioned species by significantly lacking phosphatidylcholine besides accommodating major amounts of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmonomethylethanolamine and sulfoquinovosyldiacylglycerol; moderate amounts of phosphatidylethanolamine and trace amounts of an unidentified phospholipid, an unidentified aminolipid and an unidentified phosphoglycolipid. Strain CC-AMH-0(T) possessed C18:1 ω7c and/or C18:1 ω6c (summed feature 8) as predominant fatty acids, 63.3 mol% DNA G+C content and ubiquinone-10 (Q-10) as the sole respiratory quinone. On the basis of polyphasic taxonomic evidences, strain CC-AMH-0(T) is proposed to represent a novel genus and species of the family Phyllobacteriaceae, for which the name Oricola cellulosilytica gen. nov., sp. nov. is proposed. The type strain of the type species is CC-AMH-0(T) (=JCM 19534(T) =BCRC 80694(T)). Emended descriptions of M. loti and P. myrsinacearum are also proposed.

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“…At the time of writing, the family comprised nine genera: Phyllobacterium (Knösel, 1984), Aminobacter (Urakami et al, 1992), Mesorhizobium (Jarvis et al, 1997), Aquamicrobium (Bambauer et al, 1998), Pseudaminobacter (Kämpfer et al, 1999), Defluvibacter (Fritsche et al, 1999), Nitratireductor (Labbé et al, 2004), Hoeflea (Peix et al, 2005) and Chelativorans (Doronina et al, 2010). We isolated a novel bacterium belonging to the family Phyllobacteriaceae from manure compost.…”

mentioning

confidence: 99%

Thermovum composti gen. nov., sp. nov., an alphaproteobacterium from compost

Yabe

Aiba

Sakai

et al. 2012

International Journal of Systematic and Evolutionary Microbiology

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A Gram-stain-positive thermophilic bacterium, designated strain Nis3T, was isolated from compost. The strain grew at 23–57 °C (optimum, 50 °C); no growth was observed below 15 or above 60 °C. The pH range for growth was 5.9–8.8 (optimum, 7.0); no growth was observed below pH 5.4 or above pH 9.3. The DNA G+C content of strain Nis3T was 63.4 mol%. The dominant quinone type was ubiquinone Q-10. The major fatty acids were C18 : 1ω7c, C19 : 0ω8c cyclo and C18 : 0. The polar lipids comprised phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, hydroxyphosphatidylethanolamine, phosphatidylinositol, phosphatidylmonomethylethanolamine, an unknown glycolipid and a ninhydrin-positive phospholipid. 16S rRNA gene sequence analysis assigned this bacterium to the family Phyllobacteriaceae in the Alphaproteobacteria but it shared less than 95.2 % sequence similarity with other members of the family. The chemotaxonomic and phenotypic characteristics of strain Nis3T differed in some respects from those of members of the family Phyllobacteriaceae . Therefore, strain Nis3T is considered to represent a novel species of a new genus in the family Phyllobacteriaceae , for which the name Thermovum composti gen. nov., sp. nov. is proposed. The type strain is Nis3T ( = JCM 17863T = KCTC 23707T).

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Chelativorans multitrophicus gen. nov., sp. nov. and Chelativorans oligotrophicus sp. nov., aerobic EDTA-degrading bacteria

Cited by 50 publications

References 43 publications

Nematicidal Bacteria Associated to Pinewood Nematode Produce Extracellular Proteases

Nematicidal Bacteria Associated to Pinewood Nematode Produce Extracellular Proteases

Oricola cellulosilytica gen. nov., sp. nov., a cellulose-degrading bacterium of the family Phyllobacteriaceae isolated from surface seashore water, and emended descriptions of Mesorhizobium loti and Phyllobacterium myrsinacearum

Thermovum composti gen. nov., sp. nov., an alphaproteobacterium from compost

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