Método não invasivo para avaliação da movimentação embrionária de Caiman yacare (Daudin, 1802)

Gomes, Lianna Ghisi; Stocco, Matias Bassinello; Sousa, N. P. de; Martini, Ana Carolina; Morgado, Thais Oliveira; Flôres, Fabíola Niederauer; Moreira, Leonardo Felipe Bairos; Ferraz, Rosa Helena dos Santos; Souza, Roberto Lopes de

doi:10.1590/1678-4162-11260

Cited by 1 publication

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“…Although there are other methods for evaluating the detection of embryo egg movement, such as ovoscopes or "window" making, we used the Egg Buddy ® digital monitor to detect blood pulse and embryonic movement. This reliable method, as described by Pollard et al (2016) and Gomes et al (2019), is effective, as it does not require invasive techniques that could introduce the risk of infection. Furthermore, it has been safely used in studies on endangered species of birds and reptiles and it does not require euthanasia of embryos after the completion of the study.…”

Section: Discussionmentioning

confidence: 99%

“…To administer the injections, the eggs were opened and sealed with an adhesive to limit infection. Heartbeat and embryonic movements were measured using an Egg Buddy ® egg digital monitor (Avian Biotech, Animal Genetics, England) on days 30, 35, 42, 49, 56, and 60; on these days all of the eggs were briefly removed from the incubator and placed in the egg digital monitor for 2 min (Gomes et al, 2019).…”

Section: Methodsmentioning

confidence: 99%

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Influence of incubation temperature and embryonic motility on the growth of members of Caiman yacare (Daudin, 1802)

et al. 2024

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This study aimed to evaluate whether skeletal development of the Pantanal Caiman (Caiman yacare) is similarly influenced by temperature variation and controlled increases in embryo motility. All eggs were incubated at 90% humidity and 29 °C for the first 45 days. Thereafter, the incubation temperature was either maintained at 29 °C and embryos were treated with 4-aminopyridine (4-AP) on days 46, 47, 48, and 49 (Group I, 29 °C 4-AP, n = 15); maintained at 29 °C (n = 14; Group II); or at 33 °C (n = 14, Group III). Embryonic movement was measured using an Egg Buddy® digital monitor on days 30, 35, 42, 49, 56, and 60, at which point embryos were euthanized and samples were collected for analysis. No differences were observed between groups with varying incubation temperatures. In contrast, embryonic motility was greater in embryos treated with 4-AP (P < 0.001) on day 49, and this was associated with higher proportions of snout-vent and hand lengths. This study demonstrates for the first time that pharmacologically induced increases in embryo motility result in phenotypic changes to the proportion of elements during prenatal ontogeny, thereby effectively altering the adaptation of the species to specific environments.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Influence of incubation temperature and embryonic motility on the growth of members of Caiman yacare (Daudin, 1802)

et al. 2024

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show abstract

Método não invasivo para avaliação da movimentação embrionária de Caiman yacare (Daudin, 1802)

Abstract: Método não invasivo para avaliação da movimentação embrionária de Caiman yacare (Daudin, 1802) [Non-invasive method for evaluation of embrionic moviment of Caiman yacare (Daudin, 1802)]

Cited by 1 publication

References 10 publications

Influence of incubation temperature and embryonic motility on the growth of members of Caiman yacare (Daudin, 1802)

Influence of incubation temperature and embryonic motility on the growth of members of Caiman yacare (Daudin, 1802)

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