Método para inoculação de Sclerotium rolfsii em tomateiro

Abstract

Resumo -O objetivo deste trabalho foi determinar o tipo e concentração de inóculo mais adequados para induzir a murchade-esclerócio, sem provocar ferimentos em plantas de tomateiro. Os tratamentos foram: 2, 4, 8 e 16 g de arroz colonizado L -1 incorporados ao solo; 2, 4, 6 e 8 escleródios ou discos de micélio depositados na superfície do solo. A utilização de 8 g de arroz colonizado L -1 de solo demonstrou ser a concentração e o tipo de inóculo ideal para experimentos que visem a inoculação do patógeno sem incitação de ferimento. A deposição de discos de micélio e escleródios não ocasionaram incidência da doença. Palavras-chave -Solanum lycopersicum. Lycopersicon esculentum. Produção de inóculo. Fungo de solo.Abstract -The objective of this work was to determinate type and concentration of inoculum concentration suitable to inducing southern blight in tomato plants, without causing injury. The performed treatments consisted of 2, 4, 8, and 16 g of colonized rice grains.L -1 soil; 2, 4, 6 and 8 sclerotia or mycelium disks deposited in soil surface. The incorporation of 8 g of colonized rice grains.L -1 soil proved to be the type of inoculum concentration ideal for experiments aimed to induce southern blight with no injury in tomato plants. Deposition of mycelium disks or sclerotia did not result in disease.

Control of Sclerotium rolfsii in peanut by using Cymbopogon martinii essential oil

“…The isolates were previously grown on autoclaved rice during nine days in Petri dishes and further added to a commercial substrate (Baseplant) at 72 mg.kg -1 , in pots (1 L) (Barbosa et al, 2010). Three peanut seeds previously surface-sterilized (hypochlorite solution at 1.5%) were sown in each pot and daily watered.…”

Efficacy of Trichoderma asperellum, T. harzianum, T. longibrachiatum and T. reesei against Sclerotium rolfsii

“…The soil inoculations were made following a modified method described by Barbosa et al (2010), where, 10 g kg -1 of soil of pathogen or Trichoderma colonized rice seeds were mixed to the pot soil content. The evaluation of the number of diseased plants was made weekly up to 42 days after the soil inoculation.…”