Metschnikowia koreensis sp. nov., a novel yeast species isolated from flowers in Korea.

Hong, Soon Gyu; Chun, Jongsik; Oh, Hyun Woo; Bae, Kyung Sook

doi:10.1099/00207713-51-5-1927

Cited by 30 publications

(23 citation statements)

References 15 publications

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“…The genus Metschnikowia (and anamorphs in the genus Candida) is one of the dominant taxa found in these substrates (Lachance et al, 2001;Lachance, 2011). For example, the cosmopolitan Metschnikowia reukaufii, M. gruessii and M. koreensis have been isolated repeatedly from a wide diversity of flowers and associated bee, butterfly and bird pollinators in both the Old World and the New World (Hong et al, 2001;Pozo et al, 2011;Belisle et al, 2012). Interestingly, flowers visited by a distinct pollinator guild, the beetles, harbour different, highly specific yeast communities that are not found in plant species pollinated by other animals (Marinoni & Lachance, 2004;Lachance et al, 2005;Guzmán et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Metschnikowia drakensbergensis sp. nov. and Metschnikowia caudata sp. nov., endemic yeasts associated with Protea flowers in South Africa

Vega

Guzmán

Steenhuisen

et al. 2014

International Journal of Systematic and Evolutionary Microbiology

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In a taxonomic study of yeasts recovered from nectar of flowers and associated insects in South Africa, 11 strains were found to represent two novel species. Morphological and physiological characteristics and sequence analyses of the large-subunit rRNA gene D1/D2 region, as well as the actin, RNA polymerase II and elongation factor 2 genes, showed that the two novel species belonged to the genus Metschnikowia. Metschnikowia drakensbergensis sp. nov. (type strain EBD-CdVSA09-2T = CBS 13649T = NRRL Y-63721T; MycoBank no. MB809688; allotype EBD-CdVSA10-2A = CBS13650A = NRRL Y-63720A) was recovered from nectar of Protea roupelliae and the beetle Heterochelus sp. This species belongs to the large-spored Metschnikowia clade and is closely related to Metschnikowia proteae, with which mating reactions and single-spored asci were observed. Metschnikowia caudata sp. nov. (type strain EBD-CdVSA08-1T = CBS 13651T = NRRL Y-63722T; MycoBank no. MB809689; allotype EBD-CdVSA57-2A = CBS 13729A = NRRL Y-63723A) was isolated from nectar of Protea dracomontana, P. roupelliae and P. subvestita and a honeybee, and is a sister species to Candida hainanensis and Metschnikowia lopburiensis. Analyses of the four sequences demonstrated the existence of three separate phylotypes. Intraspecies matings led to the production of mature asci of unprecedented morphology, with a long, flexuous tail. A single ascospore was produced in all compatible crosses, regardless of sequence phylotype. The two species appear to be endemic to South Africa. The ecology and habitat specificity of these novel species are discussed in terms of host plant and insect host species.

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Section: Introductionmentioning

confidence: 99%

Metschnikowia drakensbergensis sp. nov. and Metschnikowia caudata sp. nov., endemic yeasts associated with Protea flowers in South Africa

Vega

Guzmán

Steenhuisen

et al. 2014

International Journal of Systematic and Evolutionary Microbiology

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“…The sugar composition of floral nectar varies among plant species, but shows a predominance of hexoses (glucose and fructose) and the disaccharide sucrose. Several recently described Metschnikowia species (Hong et al, 2001;Kurtzman & Droby, 2001;Lachance et al, 2001a, b;Lachance & Bowles, 2002) add to the abundance of flower-associated species over those of aquatic origin, suggesting that the proliferation of terrestrial flowering plants and associated insects has markedly impacted genetic diversification in Metschnikowia. The large number of nucleotide substitutions among Metschnikowia species in the D1/D2 domain of large subunit rDNA, especially among the large-spored species, further supports the idea that the genus is highly diverged and that there are numerous undetected species.…”

Section: Introductionmentioning

confidence: 99%

Metschnikowia vanudenii sp. nov. and Metschnikowia lachancei sp. nov., from flowers and associated insects in North America

Giménez-Jurado

Kurtzman

Starmer

et al. 2003

International Journal of Systematic and Evolutionary Microbiology

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Two new species of the ascosporic yeast genus Metschnikowia were isolated from nectaries and associated muscoid flies of flowers from the common milkweed (Asclepias syriaca) in North America, and are described as Metschnikowia vanudenii [type strain=PYCC 4650. As with the previously described Metschnikowia gruessii, M. vanudenii has vegetative cells with an 'aeroplane' or cross-like configuration, produces ovoid chlamydospores and forms ellipsoidopedunculate asci with two acicular ascospores. Metschnikowia lachancei is distinguished from other Metschnikowia species by formation of club-shaped asci with 1-2 thick clavate ascospores. The phylogenetic positions of the proposed new species within Metschnikowia were determined from sequence analysis of the D1/D2 domain of 26S rDNA. The new species show low nuclear DNA relatedness with neighbouring taxa.

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“…꽃의 꿀에는 포도 당과 과당 그리고 이당류인 자당이 주로 많이 함유되었다 고 알려져 있다 (Gimnez-Jurado et al, 2003). 본 연구에서 동해안의 동해, 서해안의 대천, 남해안의 완도에서 모두 발 견된( Table 1, Table 2, Table 3) M. koreensis는 우리나라에 서 백합꽃으로부터 이미 분리되어 보고된 효모이다 (Hong et al, 2001). Pseudozyma 속에 속하는 다른 종의 효모들이 당지질을 생산한다는 특성을 갖고 있다고 보고된 바와 같 이 (Morita et al, 2007), P. rugulosa는 계면활성제로 사용할 수 있는 mannosylerythritol lipid(MEL)를 생산함이 밝혀졌 다 (Morita et al, 2006).…”

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Identification of Yeasts Isolated from Wild Flowers Collected in Coast Areas of Korea Based on the 26S rDNA Sequences

Min¹,

Lee²,

Lee³

et al. 2013

The Korean Journal of Mycology

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: Several yeast colonies were isolated from wild flowers collected from East, West and South coast areas of Korea by plating of flower suspensions on the YPD plates containing antibiotics, streptomycin and ampicillin. Polymerase chain reactions (PCR) were performed for the amplification of D1/D2 region of 26S rDNA for those colonies. PCR-amplified nucleotide sequences were compared using BLAST for their identification. As results, 27 yeast strains belonged to 15 species were isolated from wild flowers collected at Donghae, where is located in eastern coast of Korea. Also, 34 strains belonged to 17 species were isolated from wild flowers of Daecheon, where is located in western coast of Korea. In addition, 22 strains belonged to 13 species were isolated from wild flowers collected at Wando, where is located in southern coast of Korea. Among those 45 species isolated from 3 different collection sites, only 4 species including Cryptococcus laurentii, Metschnikowia koreensis, Pseudozyma rugulosa, and Rhodotorula mucilaginosa were found from all 3 different collection sites. And 5 species including Cryptococcus aureus, Cryptococcus flavus, Hanseniaspora uvarum, Pichia guilliermondii, and Rhodosporidium fluviale were overlapped from the at least 2 different collection sites. Other 23 species were found only in a specific collection sites implying that each area has distinctive yeast flora. KEYWORDS : D1/D2 region of 26S rDNA, Eastern coast, Southern coast, Western coast, Yeast identification효모는 전통적으로 전통 주류나 장류 등의 생산에 이용 되어왔다 (Kim et al., 1999). 효모는 또한 값이 저렴한 배지 에서 쉽게 배양할 수 있는 단세포 진핵생물이므로, Pichia 등은 단백질을 과량 발현시키는 시스템으로서 최근에 중요 하게 사용되고 있다 (Cai and Gros, 2003). 이밖에도 효모 는 진핵생물을 연구하기 위한 모델로서도 중요한 역할을 하고 있다 (Oslan et al., 2012). 우리나라의 자연환경에 서 식하고 있는 새로운 효모들을 분리, 동정하여 이들을 자원 화하고, 이들로부터 고부가가치의 의약 산업이나 건강 식 품산업 등에 활용할 수 있는 신소재 개발 응용연구는 매우 중요하다 (Kim et al., 2004). 식물은 효모의 공통적인 서식 처로 잘 알려져 있기 때문에, 다양한 효모들이 나무껍질, 잎, 또는 꽃에서 발견되고 있다 (Wang et al., 2008). 식물체 에 있는 효모의 다양성을 연구하기 위해 꽃, 과일 및 나무 껍질 혹은 수분에 관여하는 곤충들을 수집하여 이들로부터 효모를 분리 동정하는 연구들이 국내외에서 간헐적으로 수 행되어 왔다. 한반도에 자생하고 있는 효모들을 포괄적으 로 탐색 조사하여 발굴된 효모들을 생물자원화하기 위한

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Metschnikowia koreensis sp. nov., a novel yeast species isolated from flowers in Korea.

Abstract: NOTE

Cited by 30 publications

References 15 publications

Metschnikowia drakensbergensis sp. nov. and Metschnikowia caudata sp. nov., endemic yeasts associated with Protea flowers in South Africa

Metschnikowia drakensbergensis sp. nov. and Metschnikowia caudata sp. nov., endemic yeasts associated with Protea flowers in South Africa

Metschnikowia vanudenii sp. nov. and Metschnikowia lachancei sp. nov., from flowers and associated insects in North America

Identification of Yeasts Isolated from Wild Flowers Collected in Coast Areas of Korea Based on the 26S rDNA Sequences

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