METTL3 promotes proliferation and myogenic differentiation through m6A RNA methylation/YTHDF1/2 signaling axis in myoblasts

Zhao, Tiantian; Zhao, Rui; Yi, Xudong; Cai, Rui; Pang, Wei

doi:10.1016/j.lfs.2022.120496

Cited by 16 publications

(12 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Interestingly, YTHDF1 modulates the translation of ZNF839 and ZFP839, resulting in the acceleration of the transcription activity of Runx2-driven osteogenesis of hBMSC [ 65 ]. YTHDF1 also promotes proliferation and myogenic differentiation by recognition and increased translation of m6A-edited MyHC, MyoD, and MyoG [ 66 ]. It has been shown that YTHDF1 was up-regulated in human and rodent pulmonary hypertension samples, knockdown of YTHDF1 ameliorated PASMC proliferation, phenotypic switching, and pulmonary hypertension development via promoting the translation of MAGED1 both in vivo and in vitro [ 67 ].…”

Section: Introductionmentioning

confidence: 99%

The function and clinical implication of YTHDF1 in the human system development and cancer

Ren

Yuan

et al. 2023

Biomark Res

View full text Add to dashboard Cite

YTHDF1 is a well-characterized m6A reader protein that is essential for protein translation, stem cell self-renewal, and embryonic development. YTHDF1 regulates target gene expression by diverse molecular mechanisms, such as promoting protein translation or modulating the stability of mRNA. The cellular levels of YTHDF1 are precisely regulated by a complicated transcriptional, post-transcriptional, and post-translational network. Very solid evidence supports the pivotal role of YTHDF1 in embryonic development and human cancer progression. In this review, we discuss how YTHDF1 influences both the physiological and pathological biology of the central nervous, reproductive and immune systems. Therefore we focus on some relevant aspects of the regulatory role played by YTHDF1 as gene expression, complex cell networking: stem cell self-renewal, embryonic development, and human cancers progression. We propose that YTHDF1 is a promising future cancer biomarker for detection, progression, and prognosis. Targeting YTHDF1 holds therapeutic potential, as the overexpression of YTHDF1 is associated with tumor resistance to chemotherapy and immunotherapy.

show abstract

Section: Introductionmentioning

confidence: 99%

The function and clinical implication of YTHDF1 in the human system development and cancer

Ren

Yuan

et al. 2023

Biomark Res

View full text Add to dashboard Cite

show abstract

“…It is worth noting that IGF2BP1 stabilizes plentiful mRNA transcripts in an m 6 A-dependent manner [ 27 ]. Meanwhile, ectopic miR-193b-3p upregulated expression of METTL3 ( p value = 0.017), an m(6)A methyltransferase critical for muscle growth [ 50 ], through m6A RNA methylation/YTHDF1/2 signaling axis [ 51 ] and interacting with MRFs [ 52 , 53 ]. These suggest that m 6 A might be involved in the miR-193b-3p/IGF2BP1 pathway in promoting myoblast proliferation.…”

Section: Discussionmentioning

confidence: 99%

miR-193b-3p Promotes Proliferation of Goat Skeletal Muscle Satellite Cells through Activating IGF2BP1

Zhang

Yang

et al. 2022

IJMS

View full text Add to dashboard Cite

As a well-known cancer-related miRNA, miR-193b-3p is enriched in skeletal muscle and dysregulated in muscle disease. However, the mechanism underpinning this has not been addressed so far. Here, we probed the impact of miR-193b-3p on myogenesis by mainly using goat tissues and skeletal muscle satellite cells (MuSCs), compared with mouse C2C12 myoblasts. miR-193b-3p is highly expressed in goat skeletal muscles, and ectopic miR-193b-3p promotes MuSCs proliferation and differentiation. Moreover, insulin-like growth factor-2 mRNA-binding protein 1 (IGF2BP1) is the most activated insulin signaling gene when there is overexpression of miR-193b-3p; the miRNA recognition element (MRE) within the IGF1BP1 3′ untranslated region (UTR) is indispensable for its activation. Consistently, expression patterns and functions of IGF2BP1 were similar to those of miR-193b-3p in tissues and MuSCs. In comparison, ectopic miR-193b-3p failed to induce PAX7 expression and myoblast proliferation when there was IGF2BP1 knockdown. Furthermore, miR-193b-3p destabilized IGF2BP1 mRNA, but unexpectedly promoted levels of IGF2BP1 heteronuclear RNA (hnRNA), dramatically. Moreover, miR-193b-3p could induce its neighboring genes. However, miR-193b-3p inversely regulated IGF2BP1 and myoblast proliferation in the mouse C2C12 myoblast. These data unveil that goat miR-193b-3p promotes myoblast proliferation via activating IGF2BP1 by binding to its 3′ UTR. Our novel findings highlight the positive regulation between miRNA and its target genes in muscle development, which further extends the repertoire of miRNA functions.

show abstract

“…m6A methylation modification is a reversible process and basically the same as the methylation modification of DNA and histones, and the overall methylation level in RNA is adjusted by transmethylation and demethylation [ 11 – 13 ]. The m6A methylation-regulated proteins includes three categories, namely writers, erasers, and readers.…”

Section: Introductionmentioning

confidence: 99%

The potential role of m6A reader YTHDF1 as diagnostic biomarker and the signaling pathways in tumorigenesis and metastasis in pan-cancer

Zhu

Yang

et al. 2023

Cell Death Discov.

View full text Add to dashboard Cite

Abstractm6A is an important RNA methylation in progression of various human cancers. As the m6A reader protein, YTHDF1 is reported to accelerate m6A-modified mRNAs translation in cytoplasm. It is highly expressed in various human cancers and contributes to the progression and metastasis of cancers. YTHDF1 was closely associated with poor prognosis and also used as a molecular marker for clinical diagnosis or therapy in human cancers. It has been reported to promote chemoresistance to Adriamycin, Cisplatin and Olaparib by increasing mRNA stability of its target molecule. Moreover, it contributes to CSC-like characteristic of tumor cells and inducing the antitumor immune microenvironment. Here, we reviewed the clinical diagnostic and prognostic values of YTHDF1, as well as the molecular mechanisms of YTHDF1 in progression and metastasis of human cancers.

show abstract

METTL3 promotes proliferation and myogenic differentiation through m6A RNA methylation/YTHDF1/2 signaling axis in myoblasts

Cited by 16 publications

References 41 publications

The function and clinical implication of YTHDF1 in the human system development and cancer

The function and clinical implication of YTHDF1 in the human system development and cancer

miR-193b-3p Promotes Proliferation of Goat Skeletal Muscle Satellite Cells through Activating IGF2BP1

The potential role of m6A reader YTHDF1 as diagnostic biomarker and the signaling pathways in tumorigenesis and metastasis in pan-cancer

Contact Info

Product

Resources

About