Mice deficient for the chromosome 21 ortholog Itsn1 exhibit vesicle-trafficking abnormalities

Yu, Young‐Moon; Chu, Po‐Yin; Bowser, David N.; Keating, Damien J.; Dubach, Daphne; Harper, Ian S.; Tkalcevic, Josephine; Finkelstein, David I.; Pritchard, Melanie April

doi:10.1093/hmg/ddn378

Cited by 27 publications

(40 citation statements)

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“…Eps15 and Dap160 double mutants had a phenotype similar to that of the single Eps15 or Dap160 mutants, suggesting that Eps15 and Dap160 act at the same step during endocytosis (Koh et al, 2007). A reduced number of exocytosis events in chromaffin cells and a slowing of endocytosis in neurons were also detected in ITSN1 null mice (Yu et al, 2008).…”

Section: Itsn Proteins Are Essential Components Of Endo-and Exocytosismentioning

confidence: 82%

“…However, low levels of ITSN1-L expression were detected in adult kidney, liver and placenta (Pucharcos et al, 2001), as well as in fetal liver, lung, kidney and muscle (Tsyba et al, 2004;Kropyvko et al, 2010a). Western blot analysis of neuron lysates and lysates of glial cells from rat brain did not reveal ITSN1-S isoforms in neurons (Yu et al, 2008). However, mRNAs of these isoforms were detected in mouse thalamic neurons by single-cell RT-PCR (Kropyvko et al, 2010a).…”

Section: Structure Of Itsn Family Proteinsmentioning

confidence: 90%

“…Some of them are neuron-specific or preferentially expressed in brain. Several studies argue that ITSN1-L isoforms completely replace the ITSN1-S isoforms in neurons of mammals (Hussain et al, 1999;Yu et al, 2008); however, ITSN1-S mRNA was detected in mouse neurons (Kropyvko et al, 2010a).…”

Section: Itsn1 Function In Neuronsmentioning

confidence: 99%

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Intersectin multidomain adaptor proteins: Regulation of functional diversity

Tsyba

Nikolaienko

Dergai

et al. 2011

Gene

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Section: Itsn Proteins Are Essential Components Of Endo-and Exocytosismentioning

confidence: 82%

Section: Structure Of Itsn Family Proteinsmentioning

confidence: 90%

See 1 more Smart Citation

Intersectin multidomain adaptor proteins: Regulation of functional diversity

Tsyba

Nikolaienko

Dergai

et al. 2011

Gene

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“…In recent years, the physiological importance of endocytosis and exocytosis has been highlighted in several pathologies such as Down syndrome (DS) [6], pre-Alzheimer's disease (AD) [6], cancer [7], multi drug resistance [8][9][10] and hypertension-mediated inflammation [11,12].…”

Section: Introductionmentioning

confidence: 99%

On Some Aspects of the Thermodynamic of Membrane Recycling Mediated by Fluid Phase Endocytosis: Evaluation of Published Data and Perspectives

Rauch¹,

Pluen²,

Foster³

et al. 2009

Cell Biochem Biophys

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The theoretical and experimental description of fluid phase endocytosis (FPE) requires an asymmetry in phospholipid number between the two leaflets of the cell membrane, which provides the biomechanical torque needed to generate membrane budding. Although the motor force behind FPE is defined, its kinetic has yet to be determined. Based on a body of evidences suggesting that the mean surface tension is unlikely to be involved in endocytosis we decided to determine whether the cytosolic hydrostatic pressure could be involved, by considering a constant energy exchanged between the cytosol and the cell membrane. The theory is compared to existing experimental data obtained from FPE kinetic studies in living cells where altered phospholipid asymmetry or changes in the extracellular osmotic pressure have been investigated. The model demonstrates that FPE is dependent on the influx and efflux of vesicular volumes (i.e. vesicular volumes recycling) rather than the membrane tension of cells. We conclude that: (i) a relationship exists between membrane lipid number asymmetry and resting cytosolic pressure and (ii) the validity of Laplace's law is limited to cells incubated in a definite hypotonic regime. Finally, we discuss how the model could help clarifying elusive observations obtained from different fields and including: (a) the non-canonical shuttling of aquaporin in cells, (b) the relationship between high blood pressure and inflammation and (c) the mechanosensitivity of the sodium/proton exchanger.

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“…Studies of loss-of-function mutations of Dap160 (Drosophila ortholog of human ITSN1) demonstrated that Dap160 is required for proper formation of synaptic boutons and organization of pre-synaptic endocytic zones in neuro-muscular junctions (Koh et al, 2004;Marie et al, 2004). Additionally, disrupting the expression of ITSN1 in mice leads to alterations in vesicle trafficking in neuronal cells (Yu et al, 2008). Moreover, neurons are characterized by a specific set of ITSN1 isoforms.…”

Section: Introductionmentioning

confidence: 99%