Mice fed fish oil diet and upregulation of brown adipose tissue thermogenic markers

Abstract

FO improves metabolic profile and upregulates thermogenic markers, suggesting an elevated thermogenesis that leads to reduced BM gain.

Eicosapentaenoic Acid Potentiates Brown Thermogenesis through FFAR4-dependent Up-regulation of miR-30b and miR-378

“…6G) were confirmed in iBAT from HF ϩ FO-fed mice compared with HF ϩ PO-or HF ϩ OO-fed mice. These results are consistent with recent publications showing thermogenic activation with FO supplementation (6,7). Subsequently, we analyzed the five candidate miRNAs that were up-regulated with EPA treatment in vitro; all five miRNAs, miR-30b, miR-193b, miR-365, miR-196a, and miR-378, were significantly up-regulated in iBAT from mice fed a HF ϩ FO diet compared with HF ϩ OO-or HF ϩ PO diet-fed mice (Fig.…”

“…Despite the surge of research for identifying cellular and molecular regulators of brown fat development (2,3), the role of dietary constituents, particularly dietary fatty acids (FA), in thermogenic activation is poorly understood. Depending on the degree of desaturation and the n-6/n-3 ratio, FA differentially control adiposity, insulin sensitivity, immune response (4), and probably energy expenditure (5)(6)(7). Lately, it has been suggested that n-3 polyunsaturated FA (PUFA) stimulate beige/brown adipogenesis in primary murine adipogenic precursor cells (5) as well as C57BL/6 mice (6, 7).…”

A high-fish-oil diet prevents adiposity and modulates white adipose tissue inflammation pathways in mice

“…Secondly, these fatty acids also elevate fatty acid oxidation in the liver [23] and small intestine [24]. Besides that, it was also demonstrated that a diet rich in fish oil can increase uncoupling protein 1 in brown adipose tissue, suggesting elevated thermogenesis [25]. All these conditions can justify the diminished BM gain in the HF-FO group.…”

Pharmacological Action of a Pregnane Glycoside, Russelioside B, in Dietary Obese Rats: Impact on Weight Gain and Energy Expenditure

“…CPT-1 upregulation is well-known to play a role in obesity alleviation (Rameshreddy et al, 2018). Upregulation of both genes may be also linked to enhanced lipolysis and decreased TGs level, where the released free fatty acids are transported to the mitochondria via the activated CPT-1 enzyme, with simultaneous induction of the thermogenic activity of UCP-1 (Bargut et al, 2016). UCP-1 is known to mediate diet-induced adrenergic thermogenesis, where its ablation was found to induce obesity (Feldmann et al, 2009).…”

“…Uncoupling protein-1 (UCP-1) is expressed mainly by the brown adipose tissues. UCP-1 is sited within the inner mitochondrial membrane where it increases energy dissipation via uncoupling of mitochondrial oxidation of substrates producing heat instead of ATP (Bargut et al, 2016). Carnitine palmitoyl transferase-1 (CPT-1) is sited at the outer membrane of the mitochondria and it is the rate-determining step in fatty acid β-oxidation.…”