Mice pancreatic islets protection from oxidative stress induced by single-walled carbon nanotubes through naringin

Abstract

The growing use of carbon nanotubes (CNTs) emphasizes the importance of its potential toxic effects on the human health. Previous studies proved that CNTs caused oxidative stress and decreased cell viability. On the other hand, reactive oxygen species (ROS) and oxidative stress impaired β-cell functions and reduced the insulin secretion. However, there is not any study on the effects of CNTs on islets and β-cells. Therefore, the present study aimed to evaluate the effects of single-walled CNTs (SWCNTs) on oxidative stress in islets in addition to the protective effects of naringin (NRG) as an antioxidant . We examined the effects of SWCNTs and naringin on islets by 3,4 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay; measurement of insulin secretion, ROS, and malondialdehyde (MDA); activities of superoxide dismutase (SOD), catalase (CAT), and glutathione (GSH) peroxidase (GSH-Px); and content of GSH and mitochondrial membrane potential (MMP). The MTT assay demonstrated that decreased viability of islets cells was dose-dependent with exposure to SWCNTs. Further studies revealed that SWCNTs decreased insulin secretion and MMP, induced the formation of ROS, increased the level of MDA, and decreased the activities of SOD, GSH-Px, and CAT and content of GSH. Furthermore, the pretreatment of islets with naringin significantly reverted back these changes. These findings revealed that SWCNTs might induce the oxidative stress to pancreatic islets, causing the occurrence of diabetes, and the protective effects of naringin that was mediated by augmentation of the antioxidant defense system of islets. Our research indicated the necessity for further in vivo and in vitro researches on the effects of SWCNTs and naringin on diabetes.

Single-walled and multiwalled carbon nanotubes induce oxidative stress in isolated rat brain mitochondria

“…In priorresearch, five times higher than control levels of intracellular ROS production were seen in MWCNT-treated Rainbow Trout Liver Cell Line (RTL-W1 cells) (Simon et al, 2014). Moreover, the results of previous studies by researchers indicated that SWCNTs could induce the oxidative stress to pancreatic islets causing the occurrence of diabetes (Ahangarpour et al, 2018a). The determination of the toxic dose of MWCNTs and SWCNTs on the brain mitochondria can be a great help in terms of using these nanoparticles, especially as a medicine-bearer titles considering that the brain is a target organ for oxidative stress and according to the essential role of mitochondria in the electron transfer chain, cellular respiration, and energy production in the brain cells.…”

“…The stock suspensions were then diluted by distilled water and sonicated like before to prepare test concentrates. Three drops of Triton X-100 were then added to the volume; hence, the combination was prepared by the mitochondrial suspension (Ahangarpour et al, 2018a; Wang et al, 2011).…”

“…Different concentrations of single-walled and multiwalled nanoparticles were exposed to 1 mL of mitochondrial suspension. Concentrations of SWCNT and MWCNTs were selected according to previous studies (Ahangarpour et al, 2018a;Wang et al, 2012). In the next step, the resulting product was shaken for 60 and 30 min in a shaking incubator with 120 rotations and a temperature of 37 C. The shaken product was then centrifuged for 1 min at 4 C and 1000 Â g. The result of this step was measured for factors.…”

Do Carbon Nanotubes and Asbestos Fibers Exhibit Common Toxicity Mechanisms?

“…The first theory postulates the generation of reactive oxygen and nitrogen species (ROS/RNS) as a consequence of injury to pleural mesothelioma cells injury due to exposure to asbestos fibers [ 42 , 43 , 44 , 45 , 46 , 47 ]. Several studies also showed the formation of free radicals, accumulation of peroxidative products and depletion of cell antioxidants in the keratinocytes and bronchial epithelial cells exposed in vitro to SWCNTs [ 48 , 49 , 50 ] and MWCNTs [ 51 , 52 , 53 , 54 , 55 ]. Like asbestos, SWCNTs/MWCNTs contain high levels of Fe, Ni, Co, Mo and other transition metal impurities which are known to induce ROS/RNS formation.…”

A Review on Antidiabetic Activity of Centaurea spp.: A New Approach for Developing Herbal Remedies

“…shown versatile and attractive antidiabetic activity. Caffeic acid, a known phenolic acid compound, could protect mice pancreatic islets from oxidative stress induced by multiwalled carbon nanotubes (MWCNTs) [193]. Investigation of the effect of caffeic acid and cinnamic acid on glucose uptake in TNF-R-induced insulin-resistant hepatocytes showed that they may eliminate insulin resistance by improving insulin signaling and enhancing glucose uptake in insulin-resistant cells, which described their antihyperglycemic potential [194].…”