Micellar‐based spectrofluorimetric method for the selective determination of ledipasvir in the presence of sofosbuvir: application to dosage forms and human plasma

Abstract

A fast, low-cost, sensitive, and selective spectrofluorimetric method for the determination of ledipasvir was developed and validated. The method is based on an enhancement in the native fluorescence intensity of ledipasvir by 500% of its original value by the formation of hydrogen bonds between the cited drug and Tween-20 in the micellar system (pH = 5.0). All fluorescence measurements were carried out at 425 nm and 340 nm for emission and excitation wavelengths, respectively. A linear relationship between the concentration of ledipasvir and the observed fluorescence intensity was achieved in the range of 0.1-2.0 μg ml −1 with 0.028, 0.084 μg ml −1 , for detection and quantitation limits, respectively. The acquired selectivity and sensitivity using the proposed method facilitate the analysis of ledipasvir in spiked human plasma with sufficient percentage recovery (95.36-99.30%). The proposed method was developed and validated according to International Council for Harmonisation (ICH) guidelines. Moreover, the cited drug was successfully determined in its pharmaceutical dosage form using the proposed method. In addition, the validity of the proposed results was statistically confirmed using Student's t-test, variance ratio F-test, and interval hypothesis test. K E Y W O R D Shuman plasma, ledipasvir, pharmaceutical tablets, sofosbuvir, spectrofluorimetric, Tween-20

Micelle‐enhanced spectrofluorimetric determination of diphenhydramine: application to human plasma and its simultaneous determination with naproxen in pharmaceutical tablets

“…In general, the micellar system creates a highly viscous environment that could limit the internal movement of the enclosed drug fluorophore to minimize the possibility of nonradiative deactivation process. [ 51 ] Electrostatic attraction occurs between the negatively charged anionic surfactant, SDS, and the positively charged DPH molecules (p K a of DPH is 8.98, the drug will be positively charged at pH 7 used during this study). This will produce a further limitation of the movement of DPH molecules and increase rigidity, leading to a great enhancement in fluorescence intensity.…”

Resonance Rayleigh scattering and spectrofluorimetric approaches for the selective determination of rupatadine using erythrosin B as a probe: application to content uniformity test

“…[ 44,45 ] As the calculated t ‐ (paired) and F‐values at the 95% confidence level were less than the tabulated values, it was concluded that no significant difference among the two methods was observed. In the interval hypothesis test, true bias based on recovery experiments was evaluated using the following equation [ 44 ] : Lower limit ( θ L ) and upper limit ( θ U ) of the confidence interval were obtained: where, where x 1 and x 2 are mean values determined by the proposed and the reported methods, respectively. S p and t are the pooled standard deviation and one‐sided t ‐value at the 95% confidence level, respectively.…”

Spectrofluorimetric determination of the anti‐Covid 19 agent, remdesivir, in vials and spiked human plasma

“…The utilization of surfactants has a positive impact on analysis with respect to improvement or induction of fluorescence in addition to chromatographic resolution. [21][22][23][24][25][26] SDS above its critical micellar concentration (CMC) has shown improved sensitivity in this research work. The developed methods have numerous advantages such as being very simple without tedious sample pretreatment or analysis steps using available inexpensive solvents (distilled water or SDS) compared to Britton Robinson buffer and pH adjustment in reported spectroflourimetric method [20] and the sophisticated steps required for chromatographic or electrochemical analysis [9][10][11][12][13][14][15] that need well-trained personnel.…”