Micellar electrokinetic capillary chromatography theory based on electrochemical parameters: optimization for three modes of operation

Abstract

Two new, fundamental equations for micellar etectrokinetic capfllary chromatography (MECC) have been derived, which are analogous to the corresponding capillary zone electrophoresis (CZE) equations for the resolution and the migration time. The components In the theoretical plate height expression for MECC are compared with the appropriate parameters of the Van Deemter equation. MECC optimum resolution has been found for neutral solutes In three cases, where the migration mobility of the micelle Is negative, zero, and positive. For the first case, which Is similar to the CZE one, the condition has been found that makes the resolution approach Infinity. For the two cases of positive and zero migration mobility of micelle, the optimal ranges of the capacity factors for good resolution and resolution per unit time have been found to be between 2 and 5. These optimal ranges approximate to that for conventional column chromatography, even though the physical causes of flow are different.

New Looks at Capillary Zone Electrophoresis (CZE) and Micellar Electrokinetic Capillary Chromatography (MECC) and Optimization of MECC

“…By converting figure of Merits in MECC to electrochemical parameters 5 and pursuing similar procedure we applied to capillary electrophoresis and using effective length³ solute travels rather than length of capillary and then converting the resolution equation in terms of chromatography parameters new equation for resolution could be found which is published in another paper 7 .…”

“…There are several work which have been done to final the optimum conditions of this Nano Separation Technique 5,[8][9][10] .…”

Quantitative description of analyte migration behavior based on dynamic complexation in capillary electrophoresis with one or more additives

“…In Eq. (9), the fraction of the analyte in the form of each complex or free analyte is determined by the probability of than one additive the analyte interacting with each additive, and the coefficients of each mobility term are those probabilities. If the additives do interact, the coefficients will need to be modified according to the type of interaction.…”

“…( 6 ) or Eq. (9). Because the probability of an additive (including a micelle) interacting with two analyte molecules simultaneously is small, especially when the additive concentration is much higher than the analyte concentration, this theory can be applied to almost all additives used in CE today.…”

“…Because the probability of an additive (including a micelle) interacting with two analyte molecules simultaneously is small, especially when the additive concentration is much higher than the analyte concentration, this theory can be applied to almost all additives used in CE today. Equation (9) can be modified to apply to situations even when more complex interactions are present. Once the determining factors for the net electrophoretic mobilities are put into perspective for CE systems with one additive, the relationships of the mobilities in CE systems with more than one additive can readily be obtained (Eq.…”