Micellar Electrokinetic Capillary Electrophoresis for Rapid Analysis of Patulin in Apple Cider

Abstract

A micellar electrokinetic capillary chromatography (MECC) mode was applied to a capillary electrophoresis (CE) method, which was developed for detection and quantitation of patulin in apple ciders. This method used a small sample amount (2 mL) and consumed minimal organic solvent compared to the most commonly used HPLC methods. The sample preparation procedure of the CE method was also simpler than other chromatographic techniques developed for patulin analysis. Patulin was detected with a photodiode array detector at 273 nm. The standard curve was linear (r(2) = 0.9984) from 75 microgram/L to 121 microgram/mL with patulin working solutions corresponding to 3.8 microgram/L to 6.1 microgram/mL patulin in the sample. The linearity was better in a narrower range of concentrations (r(2) = 0.9999) from 75 microgram/L to 24.1 microgram/mL. The limit of detection of the method was 3.8 microgram/L. Patulin recoveries at 4 levels in spiked samples (10-121 microgram/L) ranged from 95.2 to 105.4%. The recoveries were 96. 9% and 99.2% for 2 levels (22.3 and 223 microgram/L, respectively) of patulin in infected apple samples. This method represents a unique alternative method for rapid and sensitive analysis of patulin in apple ciders.

Comparison between capillary electrophoresis and high performance liquid chromatography for the study of the occurrence of patulin in apple juice intended for infants

“…Indeed, micellar electrokinetic chromatography (MEKC) and capillary electrochromatography (CEC) methods have become powerful separation techniques for both neutral and charged compounds. Few studies on the use of capillary electrophoresis for the analysis of patulin have been conducted (Tsao and Zhou, 2000;MurilloArbizu et al, 2008). To date, there have been no reported attempts to compare the CE methodology with the HPLC one in the determination of this mycotoxin.…”

Patulin Analysis: Sample Preparation Methodologies and Chromatographic Approaches

“…In fact, compounds or mixtures of neutral and charged compounds can be analyzed by micellar electrokinetic capillary electrophoresis or micellar electrokinetic capillary chromatography (MECC). The method was described as having the advantage of requiring small amounts of sample and smaller amount of organic solvents when compared with HPLC (Tsao & Zhou, 2000).…”

Ocorrência, aspectos toxicológicos, métodos analíticos e controle da patulina em alimentos

“…Com o uso da cromatografia capilar micelar eletrocinética com detector de fotodiodo a 273nm, TSAO & ZHOU (2000) obtiveram recuperação de 98%, e o LD foi de 3,8µg L -1 . A CLAE-UV é um método comumente usado para determinação e quantificação de patulina, visto que essa toxina é relativamente polar e exibe amplo espectro de absorção (GÖKMEN & ACAR, 1999;YURDUM et al, 2001;GÖKMEN et al, 2005).…”