Micro 3D Culture System using Hyaluronan-Collagen Capsule for Skeletal Muscle-Derived Stem Cells~!2009-07-29~!2010-02-03~!2010-04-28~!

Tono-Okada, Kayoko; Okada, Yoshinori; Masuda, Maki; Hoshi, Akio; Akatsuka, Akira; Teramoto, Akira; Abe, Kôji; Tamaki, Toru

doi:10.2174/1875043501003010018

Cited by 4 publications

(1 citation statement)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The cell-to-cell interaction directly regulates cell differentiation in the recipient tissue environment. 51,69,70 In particular, in cases of large-volume muscle implantations for treatment of critical-sized defects (such as those from severe trauma or large tumor resections) and aesthetic purposes, preserving an appropriate cellular localization and retention is difficult due to the absence of a facial soft tissue envelope. To address these issues, a diversity of hydrogels have been used as encapsulating cell delivery vehicles since hydrogels can homogeneously incorporate cells and provide a highly hydrated environment that is amenable to the rapid diffusion of nutrients and metabolites.…”

Section: Discussionmentioning

confidence: 99%

Photopolymerizable Hydrogel-Encapsulated Fibromodulin-Reprogrammed Cells for Muscle Regeneration

Yang

Lee

et al. 2020

Tissue Engineering Part A

View full text Add to dashboard Cite

A central challenge in tissue engineering is obtaining a suitable cell type with a capable delivery vehicle to replace or repair damaged or diseased tissues with tissue mimics. Notably, for skeletal muscle tissue engineering, given the inadequate availability and regenerative capability of endogenous myogenic progenitor cells as well as the tumorigenic risks presented by the currently available pluri-and multipotent stem cells, seeking a safe regenerative cell source is urgently demanded. To conquer this problem, we previously established a novel reprogramming technology that can generate multipotent cells from dermal fibroblasts using a single protein, fibromodulin (FMOD). The yield FMOD-reprogrammed (FReP) cells exhibit exceeding myogenic capability without tumorigenic risk, making them a promising and safe cell source for skeletal muscle establishment. In addition to using the optimal cell for implantation, it is equally essential to maintain cellular localization and retention in the recipient tissue environment for critical-sized muscle tissue establishment. In this study, we demonstrate that the photopolymerizable methacrylated glycol chitosan (MeGC)/type I collagen (ColI)hydrogel provides a desirable microenvironment for encapsulated FReP cell survival, spreading, extension, and formation of myotubes in the hydrogel three-dimensionally in vitro, without undesired osteogenic, chondrogenic, or tenogenic differentiation. Furthermore, gene profiling revealed a paired box 7 (PAX7) / myogenic factor 5 (MYF5) / myogenic determination 1 (MYOD1) / myogenin (MYOG) / myosin cassette elevation in the encapsulated FReP cells during myogenic differentiation, which is similar to that of the predominant driver of endogenous skeletal muscle regeneration, satellite cells. These findings constitute the evidence that the FReP cell-MeGC/ColI-hydrogel construct is a promising tissue engineering mimic for skeletal muscle generation in vitro, and thus possesses the extraordinary potential for further in vivo validation.

show abstract

Section: Discussionmentioning

confidence: 99%

Photopolymerizable Hydrogel-Encapsulated Fibromodulin-Reprogrammed Cells for Muscle Regeneration

Yang

Lee

et al. 2020

Tissue Engineering Part A

View full text Add to dashboard Cite

show abstract

Three-dimensional differentiation of adipose-derived mesenchymal stem cells into insulin-producing cells

et al. 2015

View full text Add to dashboard Cite

The aim of this study is to evaluate the collagen/hyaluronic acid (Col/HA) scaffold effect on the differentiation of insulin-producing cells (IPCs) from adipose-derived mesenchymal stem cells (ASCs). In this experimental study, ASCs were cultured and seeded in a Col/HA scaffold (3D culture) and then treated with induction media. After induction, the presence of IPCs was evaluated using gene expression (PDX-1, GLUT-2 and insulin) analysis and immunocytochemistry, while functional maturity was determined by measuring insulin release in response to low- and high-glucose media. The induced IPCs were morphologically similar to pancreatic islet-like cells. Expression of the islet-associated genes PDX-1, GLUT-2 and insulin genes in 3D-cultured cells was markedly higher than the 2D-cultured cells exposure differentiation media. Compared to the 2D culture of ASCs-derived IPCs, the insulin release from 3D ASCs-derived IPCs showed a nearly 4-fold (p < 0.05) increase when exposed to a high glucose (25 mmol) medium. The percentage of insulin-positive cells in the 3D experimental group showed an approximately 4-fold increase compared to the 2D experimental culture cells. The results of this study demonstrated that the COL/HA scaffold can enhance the differentiation of IPCs from rat ASCs.

show abstract

Probing the role of scaffold dimensionality and media composition on matrix production and phenotype of fibroblasts

Chawla

Chameettachal

Ghosh

2015

Materials Science and Engineering: C

View full text Add to dashboard Cite

Micro 3D Culture System using Hyaluronan-Collagen Capsule for Skeletal Muscle-Derived Stem Cells~!2009-07-29~!2010-02-03~!2010-04-28~!

Cited by 4 publications

References 25 publications

Photopolymerizable Hydrogel-Encapsulated Fibromodulin-Reprogrammed Cells for Muscle Regeneration

Photopolymerizable Hydrogel-Encapsulated Fibromodulin-Reprogrammed Cells for Muscle Regeneration

Three-dimensional differentiation of adipose-derived mesenchymal stem cells into insulin-producing cells

Probing the role of scaffold dimensionality and media composition on matrix production and phenotype of fibroblasts

Contact Info

Product

Resources

About