Micro-anatomical and functional assessment of ciliated epithelium in mouse trachea using optical coherence phase microscopy

Ansari, Rehman; Buj, Christian; Pieper, Mario; König, Peter; Schweikard, Achim; Hüttmann, Gereon

doi:10.1364/oe.23.023217

Cited by 18 publications

(13 citation statements)

References 22 publications

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“…Optical coherence tomography (OCT) uses interference of broad-band light to generate cross-sectional images similar to ultrasound [11,12]. Using high NA imaging optics [13] and infrared radiation with several hundred nanometer spectral width [14], OCT with microscopic resolution (mOCT) can be used for a volumetric IVM [15][16][17]. However, one fundamental problem has to be overcome.…”

Section: Introductionmentioning

confidence: 99%

Novel endoscope with increased depth of field for imaging human nasal tissue by microscopic optical coherence tomography

Schulz‐Hildebrandt¹,

Pieper²,

Stehmar³

et al. 2018

Biomed. Opt. Express

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Intravital microscopy (IVM) offers the opportunity to visualize static and dynamic changes of tissue on a cellular level. It is a valuable tool in research and may considerably improve clinical diagnosis. In contrast to confocal and non-linear microscopy, optical coherence tomography (OCT) with microscopic resolution (mOCT) provides intrinsically cross-sectional imaging. Changing focus position is not needed, which simplifies especially endoscopic imaging. For in-vivo imaging, here we are presenting endo-microscopic OCT (emOCT). A graded-index-lens (GRIN) based 2.75 mm outer diameter rigid endoscope is providing 1.5 - 2 µm nearly isotropic resolution over an extended field of depth. Spherical and chromatic aberrations are used to elongate the focus length. Simulation of the OCT image formation, suggests a better overall image quality in this range compared to a focused Gaussian beam. Total imaging depth at a reduced sensitivity and lateral resolution is more than 200 µm. Using a frame rate of 80 Hz cross-sectional images of concha nasalis were demonstrated in humans, which could resolve cilial motion, cellular structures of the epithelium, vessels and blood cells. Mucus transport velocity was successfully determined. The endoscope may be used for diagnosis and treatment control of different lung diseases like cystic fibrosis or primary ciliary dyskinesia, which manifest already at the nasal mucosa.

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Section: Introductionmentioning

confidence: 99%

Novel endoscope with increased depth of field for imaging human nasal tissue by microscopic optical coherence tomography

Schulz‐Hildebrandt¹,

Pieper²,

Stehmar³

et al. 2018

Biomed. Opt. Express

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“…Recently, Ansari et al . utilized a high resolution phase resolved spectral domain optical microscopy system to measure cilia dynamics in the mouse airway 26 . Compared to spectral domain systems, swept-source based OCT systems offer several attractive advantages including balanced detection and potentially higher signal to noise performance at higher speeds 27 .…”

Section: Introductionmentioning

confidence: 99%

Visualization and Detection of Ciliary Beating Pattern and Frequency in the Upper Airway using Phase Resolved Doppler Optical Coherence Tomography

Jing

Chen

Chou

et al. 2017

Sci Rep

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Ciliary motion plays a critical role in the overall respiratory health of the upper airway. These cilia beat at a native frequency and in a synchronized pattern to continuously transport foreign particulate trapped in a layer of mucous out of the upper airway. Disruption of ciliary motion can lead to severe respiratory diseases and compromised respiratory function. Currently, the study of cilia requires expensive high speed cameras and high powered microscopes which is unsuitable for in vivo imaging and diagnosis. Doppler based optical coherence tomography has the potential to visualize the microscopic motion of cilia during their beating cycle. We demonstrate the development of a high-speed Doppler optical coherence tomography system that not only can rapidly determine the cilia beat frequency, but also simultaneously visualize the temporal cilia beating pattern which plays critical roles in cilia function.

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“…In particular, our group has applied μOCT to the dynamic imaging of respiratory cilia [6–8], the organelles on airway epithelia that are responsible for mucus clearance. Other OCT approaches to ciliary imaging and mucociliary transport have also been recently developed [9–11]. Clinical translation of μOCT so that it can be used for diagnostic imaging in vivo requires miniaturized probes that are small, stable, and flexible to reach a targeted area of interest.…”

mentioning

confidence: 99%

Flexible, high-resolution micro-optical coherence tomography endobronchial probe toward in vivo imaging of cilia

et al. 2017

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We report the design and fabrication of a flexible, longitudinally scanning high-resolution micro-optical coherence tomography (μOCT) endobronchial probe, optimized for micro-anatomical imaging in airways. The 2.4 mm diameter and flexibility of the probe allows it to be inserted into the instrument channel of a standard bronchoscope, enabling real-time video guidance of probe placement. To generate a depth-of-focus enhancing annular beam, we utilized a new fabrication method, whereby a hollow glass ferrule was angle-polished and gold-coated to produce an elongated annular reflector. We present validation data that verifies the preservation of linear scanning, despite the use of flexible materials. When utilized on excised, cultured mouse trachea, the probe acquired images of comparable quality to those obtained by a benchtop μOCT system.

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Micro-anatomical and functional assessment of ciliated epithelium in mouse trachea using optical coherence phase microscopy

Cited by 18 publications

References 22 publications

Novel endoscope with increased depth of field for imaging human nasal tissue by microscopic optical coherence tomography

Novel endoscope with increased depth of field for imaging human nasal tissue by microscopic optical coherence tomography

Visualization and Detection of Ciliary Beating Pattern and Frequency in the Upper Airway using Phase Resolved Doppler Optical Coherence Tomography

Flexible, high-resolution micro-optical coherence tomography endobronchial probe toward in vivo imaging of cilia

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